Molecular Nutrition

Effects of Dietary Leucine and Glutamic Acid on Fatty Acid Composition and Expression of Lipid Metabolism-Related Genes in Muscle of Finishing Pigs

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  • 1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Laboratory of Animal Nutritional Physiology and Metabolic Process, and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
    3. Evonik Degussa(China) Co., Ltd., Beijing 100600, China

Received date: 2017-11-02

  Online published: 2018-05-06

Abstract

This study was conducted to investigate the effects of dietary leucine (Leu) and glutamic acid (Glu) on fatty acid composition and expression of lipid metabolism-related genes in muscle of finishing pigs. Sixty Duroc×Large White×Landrace pigs with an average body weight about 77 kg were randomly assigned to five groups, and each group had 12 pigs (male:female=1:1). The pigs in control group were fed a basal diet, and those in the experiment groups were fed the basal diet supplemented with 2.05% L-alanine (iso-nitrogenous control group), 1.00% Leu+1.37% L-alanine (Leu group), 1.00% Glu+1.44% L-alanine (Glu group) and 1.00% Leu+1.00% Glu (Leu+Glu group), respectively. After feeding 60 days, the pigs were slaughtered and the muscle samples were collected to measure the fatty acid composition and mRNA relative expression levels of genes related to lipid metabolism. The results showed that the contents of C18:2n-6 and C20:1 in longissimus dorsi muscle in Leu group were significantly increased (P<0.05), whereas the content of C18:0 was significantly decreased compared with the control group (P<0.05); the contents of C14:0 and C16:0 in longissimus dorsi muscle in Glu group were significantly decreased (P<0.05), whereas the contents of C17:0 and C18:2n-6 were significantly increased (P<0.05). In the biceps femoris muscle, compared with the control group, the content of C16:0 in Leu group was significantly decreased (P<0.05), while the content of C18:2n-6 in Glu group was significantly increased (P<0.05). Compared with the control group, the mRNA expression level of fatty-acidtransport protein 1 in longissimus dorsi muscle was significantly up-regulated in Leu group (P<0.05), whereas the mRNA expression level of fatty-acidtransport protein 4 in longissimus dorsi and biceps femoris muscles was significantly down-regulated in Leu+Glu group (P<0.05). Compared with the iso-nitrogenous control group, the mRNA expression level of lipoprotein lipase in longissimus dorsi muscle was significantly up-regulated in Glu and Leu+Glu groups (P<0.05). These findings indicate that diets supplemented with 1.00% Leu or 1.00% Glu can regulate the fatty acid composition and the expression of genes related to lipid metabolism in muscle of finishing pigs.

Cite this article

HU Chengjun, ZHANG Ting, ZHANG Tao, YIN Yulong, KONG Xiangfeng, JIANG Qingyan . Effects of Dietary Leucine and Glutamic Acid on Fatty Acid Composition and Expression of Lipid Metabolism-Related Genes in Muscle of Finishing Pigs[J]. Chinese Journal of Animal Nutrition, 2018 , 30(5) : 1798 -1806 . DOI: 10.3969/j.issn.1006-267x.2018.05.022

References

[1] WOOD J D,RICHARDSONI R I,NUTE G R,et al.Effects of fatty acids on meat quality:a review[J].Meat Science,2004,66(1):21-32.  

[2] ROS-FREIXEDES R,REIXACH J,BOSCH L,et al.Response to selection for decreased backfat thickness at restrained intramuscular fat content in Duroc pigs[J].Journal of Animal Science,2013,91(8):3514-3521.  

[3] JEWELL J L,KIM Y C,RUSSELL R C,et al.Differential regulation of mTORC1 by leucine and glutamine[J].Science,2015,347(6218):194-198.  

[4] ZHANG Y Y,GUO K Y,ROBERT E L,et al.Increasing dietary leucine intake reduces diet-induced obesity and improves glucose and cholesterol metabolism in mice via multi mechanisms[J].Diabetes,2007,56(6):1647-1654.  

[5] FREUDENBERG A,PETZKE K J,KLAUS S.Dietary L-leucine and L-alanine supplementation have similar acute effects in the prevention of high-fat diet-induce obesity[J].Amino Acids,2013,44(2):519-528.  

[6] HYUN Y,ELLIS M,MCKEITH F K,et al.Effect of dietary leucine and lysine levels on intramuscular fat content in finishing pigs[J].Canadian Journal of Animal Science,2003,83(2):315-318.  

[7] HU C J,JIANG Q Y,ZHANG T,et al.Dietary supplementation with arginine and glutamic acid modifies growth performance,carcass traits,and meat quality in growing-finishing pigs[J].Journal of Animal Science,2017,95(6):2680-2689.

[8] 周笑犁,孔祥峰,范觉鑫,等.味精与高脂日粮对生长猪胴体性状与组成的影响[J].食品工业科技,2014,35(5):330-333,337.

[9] HU C J,JIANG Q Y,ZHANG T,et al.Dietary supplementation with arginine and glutamic acid enhances key lipogenic gene expression in growing pigs[J].Journal of Animal Science,2017,95(12):5507-5515.  

[10] NARDELLI T R,RIBEIRO R A,BALBO S L,et al.Taurine prevents fat deposition and ameliorates plasma lipid profile in monosodium glutamate-obese rats[J].Amino Acids,2011,41(4):901-908.  

[11] 胡诚军,张婷,李华伟,等.饲粮添加亮氨酸和谷氨酸对肥育猪生长性能、胴体性状和肉品质的影响[J].动物营养学报,2017,29(2):590-596.

[12] TAN B,YIN Y L,LIU Z Q,et al.Dietary L-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs[J].Amino Acids,2009,37(1):169-175.  

[13] KIM M J,PARVIN R,MUSHTAQ M M H,et al.Influence of monochromatic light on quality traits,nutritional,fatty acid,and amino acid profiles of broiler chicken meat[J].Poultry Science,2013,92(11):2844-2852.  

[14] KENNEDY A,MARTINEZ K,CHUANG C C,et al.Saturated fatty acid-mediated inflammation and insulin resistance in adipose tissue:mechanisms of action and implications[J].The Journal of Nutrition,2009,139(1):1-4.  

[15] PISCHON T,HANKINSO S E,HOTAMISLIGIL G S,et al.Habitual dietary intake of n-3 and n-6 fatty acids in relation to inflammatory markers among US men and women[J].Circulation,2003,108(2):155-160.  

[16] KALOGEROPOULOS N,PANAGIOTAKOS D B,PITSAVOS C,et al.Unsaturated fatty acids are inversely associated and n-6/n-3 ratios are positively related to inflammation and coagulation markers in plasma of apparently healthy adults[J].Clinica Chimica Acta,2010,411(7/8):584-591.

[17] KONG X F,ZHOU X L,FENG Z M,et al.Dietary supplementation with monosodium L-glutamate modifies lipid composition and gene expression related to lipid metabolism in growing pigs fed a normal-or high-fat diet[J].Livestock Science,2015,180:247-252

[18] RICHARDS M R,LISTENBERGER L L,KELLY A A,et al.Oligomerization of the murine fatty acid transport protein 1[J].Journal of Biological Chemistry,2003,278(12):10477-10483.  

[19] GUITART M,ANDREU A L,GARCÍA-ARUMI E,et al.FATP1 localizes to mitochondria and enhances pyruvate dehydrogenase activity in skeletal myotubes[J].Mitochondrion,2009,9(4):266-272.  

[20] GERTOW K,PIETILÄINEN K H,YKI-JÄRVINEN H,et al.Expression of fatty-acid-handling proteins in human adipose tissue in relation to obesity and insulin resistance[J].Diabetologia,2004,47(6):1118-1125.

[21] LIU Y Y,LI F N,HE L Y,et al.Dietary protein intake affects expression of genes for lipid metabolism in porcine skeletal muscle in a genotype-dependent manner[J].British Journal of Nutrition,2015,113(7):1069-1077.  

[22] 尹靖东.动物肌肉生物学与肉品科学[M].北京:中国农业大学出版社,2011.

[23] LAPLANTE M,SELL H,MACNAUL K L,et al.PPAR-γ activation mediates adipose depot-specific effects on gene expression and lipoprotein lipase activity-mechanisms for modulation of postprandial lipemia and differential adipose accretion[J].Diabetes,2003,52(2):291-299.  

[24] MADEIRA M S,PIRES V M,ALFAIA C M,et al.Combined effects of dietary arginine,leucine and protein levels on fatty acid composition and gene expression in the muscle and subcutaneous adipose tissue of crossbred pigs[J].British Journal of Nutrition,2014,11(9):1521-1535.

[25] 祝仁铸,尹逊河,王元虎,等.猪肌肉组织MDHLPL基因表达与肌内脂肪含量和脂肪酸组成关系的研究[J].畜牧兽医学报,2013,44(8):1182-1188.

[26] VINCENT G,ISABELLE L,JACQUES M,et al.Lipogenic enzyme activities in subcutaneous adipose tissue and skeletal muscle from neonatal pigs consuming maternal or formula milk[J].Reproduction,Nutrition,Development,2000,40(2):103-112.  
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