Interaction Effects of pH and Lipopolysaccharide or Histamine on mRNA Expression Levels of Tight Junction Proteins of Rumen Epithelium of Dairy Goats in Vitro

SUN Yanyong, GAO Min, XU Ming, SONG Liwen, LI Yang, LI Chao, CHEN Liqing, HU Honglian

doi:10.3969/j.issn.1006-267x.2018.05.024

2018 , Vol. 30 >Issue 5: 1816 - 1826

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.05.024

Molecular Nutrition

Interaction Effects of pH and Lipopolysaccharide or Histamine on mRNA Expression Levels of Tight Junction Proteins of Rumen Epithelium of Dairy Goats in Vitro

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1. College of Animal Science and Technology, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Research Institute of Animal Nutrition, Inner Mongolia Academy of Animal Sciences, Hohhot 010031, China

Received date: 2017-11-07

Online published: 2018-05-06

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Abstract

The objective of this study was to investigate the interaction effects of pH and lipopolysaccharide (LPS) or histamine (HIS) on mRNA expression levels of tight junction proteins of rumen epithelium of dairy goats in vitro. Eight healthy Saanen dairy goats with similar body weight and milk yield were used as the donors of rumen epithelium. A 3×3 two-factor experiment design was adopted. After slaughtering, rumen epithelium was collected and inserted in the middle of half chamber in a Ussing chamber. Buffer at 5 mL was added to the serosal side, and different treatments of culture mediums at 5 mL were added to the mucosal side, each treatment had 3 replicates. Experiment 1, factor 1 was pH, and was 7.4, 5.5, 5.2, respectively; factor 2 was LPS concentration, and was 0, 30 and 60 kEU/mL, respectively. In experiment 2, factor 1 was pH, and was 7.4, 5.5, 5.2, respectively; factor 2 was HIS concentration, and was 0, 0.5 and 10.0 ng/mL, respectively. The rumen epithelium was collected after cultured for 80 min, and the expression levels of Claudin-1, Claudin-4, Claudin-7, Occluding and zonula occludens-1 (ZO-1) mRNAs were measured. The results showed as follows:1) the interaction between pH and LPS had significant effects on expression levels of Claudin-1, Claudin-7 and ZO-1 mRNAs (P<0.05). Compared with pH 7.4×0 kEU/mL LPS group, the decrease of pH or the supplementation of LPS significantly decreased expression levels of Claudin-1 and Claudin-7 mRNAs (P<0.05), and tended to rise expression level of ZO-1 mRNA, while it was the highest in pH 5.2×60 kEU/mL LPS group. 2) The interaction between pH and HIS had significant effects on expression levels of Claudin-1, Claudin-7 and ZO-1 mRNAs (P<0.05). pH 5.5×0.5 ng/mL HIS group had the lowest expression level of Claudin-1 mRNA, which was not significantly different from that in pH 5.2×0.5 ng/mL HIS group (P>0.05). Compared with pH 7.4×0 ng/mL HIS group, pH 7.4×10.0 ng/mL HIS group had significantly lower expression level of Claudin-7 mRNA, which was not significantly different from that in pH 5.5×10.0 ng/mL HISgroup (P>0.05). Compared with pH 7.4×0 kEU/mL LPS group, the decrease of pH or the supplementation of HIS tended to rise expression level of ZO-1 mRNA, and that in pH 5.2×10.0 ng/mL HIS group was significantly increased (P<0.05). The results suggest that after subacute rumen acidosis (SARA) occurs, pH interacts with LPS or HIS to decrease the expression levels of tight junction proteins in the rumen epithelium, which in turn increases the permeability of rumen epithelial mucosa.

Key words： subacute rumen acidosis; dairy goats; rumen epithelium; histamine; lipopolysaccharides; Ussing chamber; tight junction proteins

Cite this article

SUN Yanyong, GAO Min, XU Ming, SONG Liwen, LI Yang, LI Chao, CHEN Liqing, HU Honglian . Interaction Effects of pH and Lipopolysaccharide or Histamine on mRNA Expression Levels of Tight Junction Proteins of Rumen Epithelium of Dairy Goats in Vitro[J]. Chinese Journal of Animal Nutrition, 2018 , 30(5) : 1816 -1826 . DOI: 10.3969/j.issn.1006-267x.2018.05.024

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