Molecular Nutrition

Expression and Application of Aflatoxin Detoxification Enzyme Gene in Bacillus subtilis

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  • College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China

Received date: 2017-11-08

  Online published: 2018-05-06

Abstract

This experiment was conducted to study the expression and application of aflatoxin detoxification enzyme (ADTZ) gene in Bacillus subtilis (B.subtilis). The ADTZ gene was transformed into wild-type B.subtilis LN genome by constructing an integrated vector of B.subtilis. The expression of ADTZ protein was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). And the recombinant B.subtilis were inoculated in moldy corn as fermentation strains for the fermentation experiment, and the content of aflatoxin B1 (AFB1) in moldy corn samples was detected to validate the expression effect of ADTZ gene in B.subtilis. The results showed that the ADTZ gene was successively integrated into wild type B.subtilis genome by constructing integrative expression vector. The recombined B.subtilis could secrete the specific protein and it was detected by SDS-PAGE. The content of AFB1 in the fermented moldy corn of recombined B.subtilis had significant difference with the fermented moldy corn of uninoculated and wild type B.subtilis LN(P < 0.05). It showed that the ADTZ gene is successfully integrated into wild type B.subtilis LN and expressed in extracellular. The expressed product has biological activity, and can degrade AFB1 effectively.

Cite this article

SHI Dunsheng, NIE Libo, SONG Yangyang, LI Yuanxiao, LI Wang . Expression and Application of Aflatoxin Detoxification Enzyme Gene in Bacillus subtilis[J]. Chinese Journal of Animal Nutrition, 2018 , 30(5) : 1981 -1987 . DOI: 10.3969/j.issn.1006-267x.2018.05.043

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