Molecular Nutrition

Effects of Deoxynivalenol and Zearalenone on Promoting Proliferation and Promoting Migration of Cancer Cells

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  • 1. Key Laboratory of Agro-Food Safety and Quality, Ministry of Agriculture, Institute of Quality Standard & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China

Received date: 2017-11-14

  Online published: 2018-05-06

Abstract

This experiment was conducted to study the effects of deoxynivalenol (DON) and zearalenone (ZEN) on promoting proliferation and promoting migration of human colon carcinoma cell (HCT116 cell), human lung cancer cell (A549 cell) and human hepatocellular carcinoma cell (HepG2 cell). The experiment set a blank control group, and ochratoxin A (OTA) was set up as a positive control, methyl thiazolyl tetrazolium (MTT) colorimetry and cell wound healing experiment were used to determine cell viability and cell migration activity. The results showed that compared with the blank control group, 0.080 and 0.400 μmol/L OTA could significantly enhance the viability of HCT116, A549 and HepG2 cells (P<0.05 or P<0.01), 50.000 μmol/L OTA could significantly decrease the viability of HCT116, A549 and HepG2 cells (P<0.01); DON had no effect on the viability of HCT116 and A549 cells at low concentration (0.016 and 0.080 μmol/L) (P > 0.05), but DON could significantly enhance the viability HepG2 cell at low concentration (0.016 and 0.080 μmol/L) (P<0.01), and DON could significantly decrease the viability of HCT116, A549 and HepG2 cells at middle and high concentration (2.000, 10.000 and 50.000 μmol/L) (P<0.01); ZEN could significantly enhance the viability of HepG2 cell at the concentration of 0.016 to 50.000 μmol/L (P<0.05 or P<0.01), ZEN could significantly decrease the viability of HCT116 and A549 cells at high concentration (50.000 μmol/L) (P<0.01). The cell wound healing experiment results showed that compared with the blank control group, 1 and 10 nmol/L OTA, DON and ZEN could significantly promote the migration of HepG2 cell at 24 and 48 h (P<0.01). In conclusion, at the concentration of 0.016 to 50.000 μmol/L, DON and ZEN significantly promote proliferation and migration of HepG2 cell, which suggests that DON and ZEN exhibit carcinogenesis-like properties in HepG2 cell.

Cite this article

QIU Siqi, XU Zhenzhen, SHEN Hong, CHEN Ailiang, YANG Shuming, CHAO Yuzhu . Effects of Deoxynivalenol and Zearalenone on Promoting Proliferation and Promoting Migration of Cancer Cells[J]. Chinese Journal of Animal Nutrition, 2018 , 30(5) : 1988 -1995 . DOI: 10.3969/j.issn.1006-267x.2018.05.044

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