Molecular Nutrition

Effects of Citric Acid on Contents of Triglyceride and Its Metabolites of Mouse Adipocytes

Expand
  • 1. Collage of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
    2. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Urumqi 830052, China

Received date: 2017-11-09

  Online published: 2018-05-06

Abstract

The purpose of this study was to investigate the effects of citric acid on contents of triglyceride and its metabolites of mouse adipocytes. Firstly inoculated the mouse 3T3-L1 cells into mature adipocytes as the test subject, the cells were cultured in complete mediums containing 0 (CK group), 20 (test group Ⅰ), 50 (test group Ⅱ)and 200 μmol/L citric acid (test group Ⅲ) on the 14th day, respectively. The cells were collected at 0, 36 and 72 h for the detection of key rate-limiting enzyme contents in TG synthesis and catabolism, and TG metabolite contents, respectively. The results showed as follows:at 36 an 72 h, TG content in test groups Ⅰ, Ⅱ and Ⅲ was significantly higher than that in CK group(P < 0.05). The contents of aldolase (FDA), acetyl-CoA carboxylase (ACC) and fatty acid synthetase (FAS)in test groups were significantly higher than those in CK group(P < 0.05 or P < 0.01). The contents of hormone-sensitive lipase (HSL), carnitine palmityl transferase 1 (CPT1), free fatty acids (FFA) and acetyl CoA in test groups were significantly lower than those in control group(P < 0.01). In conclusion, citric acid can promote the synthesis and deposition of TG of mouse adipocytes, significantly increase the contents of FDA, ACC,and FAS in TG synthesis, and significantly reduce the contents of HSL, CPT1, FFA, acetyl CoA in TG disintegration; the optimal dosage of citric acid is 20 μmol/L.

Cite this article

REN Wanping, SHAO Wei, LUO Chenglong, YU Xiong . Effects of Citric Acid on Contents of Triglyceride and Its Metabolites of Mouse Adipocytes[J]. Chinese Journal of Animal Nutrition, 2018 , 30(5) : 2006 -2013 . DOI: 10.3969/j.issn.1006-267x.2018.05.046

References

[1] 邹思湘.动物生物化学[M].4版.北京:中国农业出版社,2017.

[2] 赵俭.不同真菌来源的ATP-柠檬酸裂解酶基因的克隆与表达[D].硕士学位论文.武汉:华中农业大学,2011:2-9.

[3] 杨文洲,薛永常,农晓帆,等.柠檬酸对啤酒酵母TCA循环中有机酸的影响[J].大连轻工业学院学报,2007,26(4):313-315.

[4] 田静.中国西门塔尔牛生产性能测定及CS基因多态性与肉质性状的关联分析[D].博士学位论文.长春:吉林大学,2012:3-21.

[5] 郭秀玲,徐民岗,张秀丽等.小鼠3T3-L1前脂肪细胞培养与诱导分化方法的建立[J].中国药物与临床,2013,12(13):1542-1545,1662.

[6] 闫莉,许言午,王晓梁,等.双抗体夹心法检测3-硝基酪氨酸方法的建立及应用[J].中国应用生理学杂志,2009,25(4):569-572.

[7] KHU L Y,STOREY K B,RUBSTOV A M,et al.Regulation of glucokinase activity in liver of hibernating ground squirrel Spermophilus undulatus[J].Biokhimiya,2014,79(7):727-732.

[8] 门丽媛,刘帅,宋士一,等.达乌尔黄鼠育肥过程和冬眠期白色脂肪组织糖代谢相关基因的差异表达[J].兽类学报,2015,35(4):422-430.

[9] 程钰蓉,孙志杰,崔球.丙酮酸脱氢酶竞争性抑制剂调控裂殖壶菌脂肪酸合成的研究[J].食品工业科技,2016,37(16):161-166.

[10] MAO J Q,DEMAYO F J,LI H G,et al.Liver-specific deletion of acetyl-CoA carboxylase 1 reduces hepatic triglyceride accumulation without affecting glucose homeostasis[J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(22):8552-8557.  

[11] BARBER M C,PRICE N T,TRAVERS M T.Structure and regulation of acetyl-CoA carboxylase genes of metazoa[J].Biochimica et Biophysica Acta:Molecular and Cell Biology of Lipids,2005,1733(1):1-28.  

[12] DENTIN R,BENHARMED F,PEGORIER J P,et al.Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation[J].Journal of Clinical Investigation,2005,115(10):2843-2854.  

[13] LORENTE-CEBRIÁN S,KULYTÉ A,HEDÉN P,et al.Relationship between site-spcific HSL phosphorylation and adipocyte lipocyte in obese women[J].Obesity Facts,2011,4(5):365-371.  

[14] CHONG X,HE J H,JIANG H F,et al.Direct effect of glucocorticoids on lipolysis in adipocytes[J].Molecular Endocrinology,2009,23(8):1161-1170.  

[15] CORNACIU I,BOESZOERMENYI A,LINDERMUTH H,et al.The minimal domain of adipose triglyceride (ATGL) ranges until leucine 254 and can be activated and inhibited by cgi-58 and GS respectively[J].PLoS One,2011,6(10):26349.

[16] CHAKRABARTI P,KANDROR K V.FoxO1 controls insulin-dependent adipose triglyceride lipase (ATGL) expression and lipolysis in adipocytes[J].Journal of Biological Chemistry,2009,284(20):13296-13300.  

[17] SERR J,SUH Y,LEE K.Regulation of adipose triglyceride lipase by fasting and refeeding in avian species[J].Poultry Science,2009,88(12):2585-2591.  

[18] 袁禹惠.饲料中脂肪及花生四烯酸水平对半滑舌鳎(Cynoglossus semilaevis)幼鱼生长、脂肪酸组成及代谢相关基因表达的影响[D].硕士学位论文.青岛:中国海洋大学,2015:2-9.

[19] 董婧.肉碱对鲤鱼幼鱼脂肪酸代谢调控作用的研究[D].博士学位论文.长春:吉林农业大学,2014:2-6.

[20] ABU-ELHEIGA L,MATZUK M M,ABO-HASHEMA K A,et al.Continuous fatty acid oxidation and reduce fat storage in mice lacking acetyl-CoA carboxylase 2[J].Science,2001,291:2613-2616.

[21] 童晋.油菜柠檬酸合酶与柠檬酸裂解酶基因克隆及功能研究[D].博士学位论文.北京:中国农业科学院,2009:3-21.

[22] 杨竹青,瞿明仁,赵向辉,等.烟酸对反刍动物脂类代谢和肉质的影响及其作用机制[J].动物营养学报,2013,25(6):1150-1157.

[23] WANG Y X,JONES V B,URS S,et al.The human fatty acid synthase gene and de novo lipogenesis are coordinately regulated in human adipose tissue[J].The Journal of Nutrition,2004,134(5):1032-1038.  

[24] 王倩倩,杨彪,夏丽丽,等.鹅乙酰辅酶A酰基转移酶2基因的克隆及其在鹅肥肝形成过程中的表达变化[J].畜牧兽医学报,2016,47(4):700-708.
Outlines

/