Effects of Diet Supplemented with 5-Hydroxytryptophan, Rumen Protected 5-Hydroxytryptophan on Plasma 5-Hydroxytryptophan and Melatonin Contents of Sheep

ZHAO Fang, GAO Chao, WANG Gen, ZHAO Guodong, LI Xiaobin, MA Chen, YANG Kailun

doi:10.3969/j.issn.1006-267x.2018.06.017

2018 , Vol. 30 >Issue 6: 2143 - 2154

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.06.017

Molecular Nutrition

Effects of Diet Supplemented with 5-Hydroxytryptophan, Rumen Protected 5-Hydroxytryptophan on Plasma 5-Hydroxytryptophan and Melatonin Contents of Sheep

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Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2017-11-22

Online published: 2018-06-20

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Abstract

This experiment aimed to study the effects of diet supplemented with 5-hydroxytryptophan, rumen protected 5-hydroxytryptophan on plasma 5-hydroxytryptophan and melatonin contents of sheep, and to explore the possibility of regulating sheep melatonin secretion mode and content by 5-hydroxytryptophan. Eighteen 1-year-old Dorset sheep with the average body weight of (55.78±3.24) kg were divided into 3 groups according to the body weight, being control group, trial group Ⅰ and trial group Ⅱ. Each sheep in all group was fed with 10% of body weight powder concentrate, 0.6 kg corn silage every day, and had free continuous access to mixed hay, additionally, each sheep in trail group Ⅰ and trial group Ⅱ was fed with 5-hydroxytryptophan (50 mg/kg BW) and rumen protected 5-hydroxytryptophan (111 mg/kg BW), respectively. The supplementary feeding experiment lasted for 15 days. The results showed that plasma 5-hydroxytryptophan content in trial groups were higher than that in control group after feeding in the morning and afternoon, and 5-hydroxytryptophan content in trial groups showed a fluctuating increasing trend after feeding in the afternoon, but the difference between two trial groups was not significant (P>0.05). Plasma tryptophan content in all groups was increased at 1.5 h after feeding in the morning, but it was decreased at 1.5 to 6.0 h after feeding in the afternoon. During the daytime, there was no significant difference in plasma 5-hydroxytryptamine content among all groups (P>0.05). The content of 5-hydroxytryptamine in each group was volatility increased at 1.5 to 6.0 h after feeding in the afternoon. Plasma melatonin content in trail group Ⅰ and trial group Ⅱ was higher than that in control group at 3.0 to 9.0 h after feeding in the morning. Among them, the differences between two trial groups and control groups at 6.0 h after feeding in the morning were significant (P<0.01). Plasma melatonin content in all groups showed a fluctuating increasing trend at 1.5 to 9.0 h after feeding in the afternoon, it in trial groups was significantly higher than that in control group at 1.5, 3.0 and 6.0 h after feeding in the afternoon (P<0.01), and it in trial group Ⅱ was significantly higher than that in control group at 9.0 h after feeding in the afternoon (P<0.05). In conclusion, diet supplemented with 5-hydroxytryptophan (50 mg/kg BW) or rumen protected 5-hydroxytryptophan (111 mg/kg BW) both can significantly elevate sheep plasma 5-hydroxytryptophan content, change melatonin secretion pattern and content in the daytime, and rumen protected 5-hydroxytryptophan shows a better effect, but plasma tryptophan and 5-hydroxytryptamine contents are not be affected significantly.

Key words： sheep; melatonin; 5-hydroxytryptophan; 5-hydroxytryptamine

Cite this article

ZHAO Fang, GAO Chao, WANG Gen, ZHAO Guodong, LI Xiaobin, MA Chen, YANG Kailun . Effects of Diet Supplemented with 5-Hydroxytryptophan, Rumen Protected 5-Hydroxytryptophan on Plasma 5-Hydroxytryptophan and Melatonin Contents of Sheep[J]. Chinese Journal of Animal Nutrition, 2018 , 30(6) : 2143 -2154 . DOI: 10.3969/j.issn.1006-267x.2018.06.017

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