Effects of Feeding Restriction on Blood Biochemical Indexes and Lipid Metabolism of Visceral Adipose Tissue in Mid-Pregnancy Dams

YANG Hong, ZHOU Xiaoling, YAN Qiongxian, TAN Zhiliang

doi:10.3969/j.issn.1006-267x.2018.06.021

2018 , Vol. 30 >Issue 6: 2182 - 2193

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.06.021

Molecular Nutrition

Effects of Feeding Restriction on Blood Biochemical Indexes and Lipid Metabolism of Visceral Adipose Tissue in Mid-Pregnancy Dams

Expand

1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. College of Animal Science, Tarim University, Alaer 843300, China;
4. Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
5. Hunan CoInnovation Center of Animal Production Safety, Changsha 410128, China

Received date: 2017-12-04

Online published: 2018-06-20

Fold

Abstract

This experiment was conducted to investigate the effects of feeding restriction on blood biochemical indexes, fatty acid composition and gene expressions related to lipid metabolism of visceral adipose tissue (VAT) in mid-pregnancy ewes. Sixteen Xiangdong black goats[gestation time of (45±3) d], body weight of (29.86±3.07) kg] were chosen and randomly assigned to control group (C group, 100% of pregnancy nutritional requirements) and restricted group (R group, 40% of pregnancy nutritional requirements), and each group comprised of 8 dams. The experiment period was 45 to 100 d of gestation. At day 101 of gestation, blood biochemical indexes, fatty acid composition and gene expressions related to lipid metabolism and energy sensing in omental, mesenteric and perirenal adipose tissues were detected. The results showed that R group compared with C group:1) blood contents of glucagon and free fatty acid were significantly increased (P<0.05), and blood contents of leptin, adiponectin and high-density lipoprotein cholesterol were significantly decreased (P<0.05). 2) The contents of arachidonic acid (C20:4n6), palmitoleic acid (C16:1), linoleic acid (C18:2n6c), epoxyeicosatrienoic acids (C20:3n6) and polyunsaturated fatty acid (PUFA) in omental adipose tissue were significantly decreased (P<0.05); the content of myristic acid in mesenteric adipose tissue was significantly decreased (P<0.05); the contents of linoleic acid, arachidonic acid, epoxyeicosatrienoic acids and PUFA in perirenal adipose tissue were significantly decreased (P<0.05). 3) The expression of adenosine 5'-monophosphate-activated protein kinase α2 (AMPKα2) gene in omental adipose tissue tended to be increased (0.05 ≤ P ≤ 0.10); the expressions of fatty acid synthetase (FASN), stearoyl-CoA desaturase-1 (SCD1) and uncoupling protein 2 (UCP2) genes in mesenteric adipose tissue tended to be decreased (0.05 ≤ P ≤ 0.10), and the expression of peroxisome proliferator-activated receptor γ (PPARγ) gene tended to be increased (0.05 ≤ P ≤ 0.10); in perirenal adipose tissue, the expressions of FASN and UCP2 genes were significantly decreased (P<0.05), while that of adenosine 5'-monophosphate-activated protein kinase β1 (AMPKβ1) gene was significantly increased (P<0.05), and that of carnitine palmitoyltransferase 1A (CPT1A) gene tended to be increased (0.05 ≤ P ≤ 0.10). These results indicate that feeding restriction during mid-gestation decreases contents of lipid metabolism regulator factors (leptin and adiponectin), increases contents of lipid metabolites (free fatty acids), and affects fatty acid composition (decreases PUFA content), down-regulates gene (FASN and SCD1) expressions related to lipid synthesis, up-regulates gene (CPT1A) expression related to lipid mobilization, and down-regulates gene (UCP2) expression related to basal energy metabolism in VAT of dams, thereby attenuates lipid anabolism and enhances lipid mobilization in VAT to regulate body energy balance.

Key words： feeding restriction; mid-gestation; fatty acids; lipid metabolism; goat

Cite this article

YANG Hong, ZHOU Xiaoling, YAN Qiongxian, TAN Zhiliang . Effects of Feeding Restriction on Blood Biochemical Indexes and Lipid Metabolism of Visceral Adipose Tissue in Mid-Pregnancy Dams[J]. Chinese Journal of Animal Nutrition, 2018 , 30(6) : 2182 -2193 . DOI: 10.3969/j.issn.1006-267x.2018.06.021

References

[1] GUZMÁN C,CABRERA R,CÁRDENAS M,et al.Protein restriction during fetal and neonatal development in the rat alters reproductive function and accelerates reproductive ageing in female progeny[J].Journal of Physiology,2006,572(1):97-108.

[2] ROOS S,LAGERLÖF O,WENNERGREN M,et al.Regulation of amino acid transporters by glucose and growth factors in cultured primary human trophoblast cells is mediated by mTOR signaling[J].American Journal of Physiology Cell Physiology,2009,297(3):C723-C731.

[3] ROSARIO F J,JANSSON N,KANAI Y,et al.Maternal protein restriction in the rat inhibits placental insulin,mTOR,and STAT3 signaling and down-regulates placental amino acid transporters[J].Endocrinology,2011,152(3):1119-1129.

[4] BARCROFT J.Researches on pre-natal life[M].Oxford:Blackwell Scientific Publications,1946.

[5] VIRTUE S,VIDAL-PUIG A.Adipose tissue expandability,lipotoxicity and the metabolic syndrome-an allostatic perspective[J].Genes & Nutrition,2007,2(1):41-45.

[6] NIELSEN M O,KONGSTED A H,THYGESEN M P,et al.Late gestation undernutrition can predispose for visceral adiposity by altering fat distribution patterns and increasing the preference for a high-fat diet in early postnatal life[J].British Journal of Nutrition,2013,109(11):2098-2110.

[7] DE BLASIO M J,GATFORD K L,ROBINSON J S,et al.Placental restriction of fetal growth reduces size at birth and alters postnatal growth,feeding activity,and adiposity in the young lamb[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2007,292(2):R875-R886.

[8] KHANAL P,HUSTED S V,AXEL A M,et al.Late gestation over-and undernutrition predispose for visceral adiposity in response to a post-natal obesogenic diet,but with differential impacts on glucose-insulin adaptations during fasting in lambs[J].Acta Physiologica,2013,210(1):110-126.

[9] 张宏福.动物营养参数与饲养标准[M].2版.北京:中国农业出版社,2010.

[10] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社,2003.

[11] ICHIHARA K,SHIBAHARA A,YAMAMOTO K,et al.An improved method for rapid analysis of the fatty acids of glycerolipids[J].Lipids,1996,31(5):535-539.

[12] WAJCHENBERG B L.Subcutaneous and visceral adipose tissue:their relation to the metabolic syndrome[J].Endocrine Reviews,2000,21(6):697-738.

[13] HABEGGER K M,HEPPNER K M,GEARY N,et al.The metabolic actions of glucagon revisited[J].Nature Reviews Endocrinology,2010,6(12):689-697.

[14] EHRHARDT R A,SLEPETIS R M,BELL A W,et al.Maternal leptin is elevated during pregnancy in sheep[J].Domest Anim Endocrinol,2001,21(2):85-96.

[15] NEDVÍDKOVÁ J,SMITKA K,KOPSKÝ V,et al.Adiponectin,an adipocyte-derived protein[J].Physiological Research,2005,54(2):133-140.

[16] VASSEUR F,LEPRÊTRE F,LACQUEMANT C,et al.The genetics of adiponectin[J].Current Diabetes Reports,2003,3(2):151-158.

[17] DIEZ J J,IGLESIAS P.The role of the novel adipocyte-derived hormone adiponectin in human disease[J].European Journal of Endocrinology,2003,148(3):293-300.

[18] HARRIS R B S,APOLZAN J W.Changes in glucose tolerance and leptin responsiveness of rats offered a choice of lard,sucrose,and chow[J].American Journal of Physiology:Regulatory, Integrative and Comparative Physiology,2012,302(11):R1327-R1339.

[19] HAGHIAC M,BASU S,PRESLEY L,et al.Patterns of adiponectin expression in term pregnancy:impact of obesity[J].The Journal of Clinical Endocrinology & Metabolism,2014,99(9):3427-3434.

[20] 茅慧玲,刘建新.反刍动物肌肉脂肪酸营养调控研究进展[J].饲料工业,2010,31(23):30-34.

[21] BANSKALIEVA V V,SAHLU T,GOETSCH A L.Fatty acid composition of goat muscles and fat depots:a review[J].Small Ruminant Research,2000,37(3):255-268.

[22] 双金,敖力格日玛,敖长金.苏尼特羊体脂脂肪酸组成的研究[J].畜牧兽医学报,2015,46(8):1363-1374.

[23] DERVISHI E,SERRANO C,JOY M,et al.Effect of the feeding system on the fatty acid composition,expression of the Δ9-desaturase,peroxisome proliferator-activated receptor alpha,gamma,and sterol regulatory element binding protein 1 genes in the semitendinous muscle of light lambs of the Rasa aragonesa breed[J].BMC Veterinary Research,2010,6:40.

[24] DERVISHI E,SERRANO C,JOY M,et al.The effect of feeding system in the expression of genes related with fat metabolism in semitendinous muscle in sheep[J].Meat Science,2011,89(1):91-97.

[25] 蒋金航,马云,王新庄.PPARγ基因调控脂肪细胞分化的研究进展[J].中国畜牧杂志,2014,50(9):91-95.

[26] YEON S H,LEE S H,CHOI B H,et al.Genetic variation of FASN is associated with fatty acid composition of Hanwoo[J].Meat Science,2013,94(1):133-138.

[27] MENENDEZ J A,LUPU R.Fatty acid synthase-catalyzed de novo fatty acid biosynthesis:from anabolic-energy-storage pathway in normal tissues to jack-of-all-trades in cancer cells[J].Archivum Immunologiae et Therapiae Experimentalis,2004,52(6):414-426.

[28] PATON C M,NTAMBI J M.Biochemical and physiological function of stearoyl-CoA desaturase[J].American Journal of Physiology Endocrinology and Metabolism,2009,297(1):E28-E37.

[29] HOLM C.Molecular mechanisms regulating hormone-sensitive lipase and lipolysis[J].Biochemical Society Transactions,2003,31(6):1120-1124.

[30] PUIGSERVER P,WU Z D,PARK C W,et al.A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis[J].Cell,1998,92(6):829-839.

[31] 孙亮,朱小泉,王沥,等.核辅激活因子PGC-1表达的分子调控机制[J].中国生物化学与分子生物学报,2005,21(4):431-439.

[32] 张艳芳.CPT1基因对猪脂肪沉积的影响及其调控机制的研究[D].博士学位论文.杭州:浙江大学,2010.

[33] BARTELDS B,TAKENS J,SMID G B,et al.Myocardial carnitine palmitoyltransferase Ⅰ expression and long-chain fatty acid oxidation in fetal and newborn lambs[J].American Journal of Physiology:Heart and Circulatory Physiology,2004,286(6):H2243-H2248.

[34] MCINNES K J,BROWN K A,HUNGER N I,et al.Regulation of LKB1 expression by sex hormones in adipocytes[J].International Journal of Obesity,2012,36(7):982-985.

[35] WU B,DU Y,LIU C,et al.Effect of repeated fasting/refeeding on body weight control and energy balance regulation in rats[J].Journal of Hygiene Research,2010,39(5):601-605.

[36] MOON J S,LEE S,PARK M A,et al.UCP2-induced fatty acid synthase promotes NLRP3 inflammasome activation during sepsis[J].Journal of Clinical Investigation,2015,125(2):665-680.

[37] DE QUEIROZ K B,GUIMARÃES J B,COIMBRA C C,et al.Endurance training increases leptin expression in the retroperitoneal adipose tissue of rats fed with a high-sugar diet[J].Lipids,2014,49(1):85-96.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References