Molecular Nutrition

Requirement of Dietary Protein for Gibel Carp (Carassius auratus gibelio) Suffered from Long-Term Starvation

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  • Institute of Fisheries, Anhui Academy of Agricultural Sciences, Hefei 230031, China

Received date: 2017-11-23

  Online published: 2018-06-20

Abstract

This study was conducted to obtain the requirement level of dietary protein for juvenile gibel carp (Carassius auratus gibelio) suffered from long-term starvation and its adaptive strategies. The juvenile gibel carp[(11.6±0.4) g] were fasted for 90 d under (15.2±2.0)℃ of water temperature, and then diets with protein levels of 20% (P20), 25% (P25), 30% (P30), 35% (P35) and 40% (P40) were provided (each replicate had 3 replicates, and each replicate had 40 carps), respectively. The re-feeding trial lasted 56 d under (24.4±2.2)℃ of water temperature. Variations of body measurements, viscera index, body composition and activities of intestinal digestive enzymes and were measured during starvation and re-feeding periods. The results showed as follows:1) after 90 d of starvation, body weight was significantly decreased (P<0.05), liver index and intestinal tract length index were significantly decreased (P<0.05), activities of intestinal digestive enzymes were significantly decreased (P<0.05), body moisture and ash content were significantly decreased (P<0.05), and body crude protein and crude lipid were significantly decreased (P<0.05). 2) After re-feeding, on day 14, there was no significant difference on body weight among groups (P>0.05); on day 28, body weight of P20 group was significantly lower than that of P30, P35 and P40 groups (P<0.05); on day 42 and 56, body weight of P35 and P40 groups was significantly higher than that of the other groups (P<0.05), and specific growth rate (SGR) and feed conversion ratio (FCR) in these two groups were close. 3) On day 56 of re-feeding, body crude protein content of P20 group was significantly lower than that of the other groups (P<0.05), body crude lipid content of P20 and P25 groups was significantly lower than that of the other groups (P<0.05), body ash content of P20 and P25 groups was significantly higher than that of the other groups (P<0.05), and body moisture content of P20 and P25 groups was significantly higher than that of P35 and P40 groups (P<0.05). 4) After re-feeding, activities of intestinal digestive enzymes were gradually increased; protease activity was increased with the improvement of dietary protein level, P35 and P40 groups were significantly higher than the other groups on day 28 (P<0.05), and P40 group was significantly higher than the other groups on day 56 (P<0.05); amylase activity was decreased with the improvement of dietary protein level, P35 and P40 groups were significantly lower than the other groups on day 28 (P<0.05), and P30, P35 and P40 groups were significantly lower than the other groups on day 56 (P<0.05). In conclusion, there are differences in utilization and adaptive process to diets with different dietary protein levels for juvenile gibel carp suffered from long-term starvation; at the early stage of re-feeding, the growth is not affected by dietary protein level, but high dietary protein level as 35% to 40% will promote the growth after recovery of ingestion and digestive function, and the theoretical optimal dietary protein level is 38.1%.

Cite this article

WU Benli, HUANG Long, HE Jixiang, CHEN Jing, SONG Guangtong, WU Song . Requirement of Dietary Protein for Gibel Carp (Carassius auratus gibelio) Suffered from Long-Term Starvation[J]. Chinese Journal of Animal Nutrition, 2018 , 30(6) : 2215 -2225 . DOI: 10.3969/j.issn.1006-267x.2018.06.025

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