Molecular Nutrition

Effects of Yeast Extract Powder on Triglyceride Content and Uric Acid Metabolism in Hemolymph of Silkworms

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  • 1. Hebei Universities R & D Center for Sericulture and Specialty Enabling Technologies, Chengde 067000, China;
    2. Institute of Sericulture, Chengde Medical University, Chengde 067000, China;
    3. Zhengding Agro-Tech Extension Station of Hebei, Shijiazhuang 050800, China

Received date: 2017-11-08

  Online published: 2018-06-20

Abstract

This experiment was conducted to investigate the effects of yeast extract powder (YEP) on the content of triglyceride and the metabolism of uric acid (UA) in hemolymph of silkworms (Bombyx mori). The silkworm variety "Jingsong×Haoyue" was used as experimental material. Choosing similar size and development of 600 silkworms randomly divided into four groups with each group had three replicates and each replicate had 50 silkworms (half male and half female). Fifth instar larvae of silkworms in experimental groups (groups JM1, JM2 and JM3) were fed fresh mulberry leaves painted with 1%, 2% and 4% YEP solution,respectively, and those in control group were fed fresh mulberry leaves painted with distilled water. The UA content, triglyceride (TG) content and xanthine oxidase (XOD) activity in hemolymph of silkworms were detected after feeding with different concentrations of YEP for 3, 4 and 5 days. The results showed that the body weight growth rate of silkworms increased significantly after feeding with different concentrations of YEP for 5 days compared with the control group (P<0.05), but no significant differences were found among experimental groups (P>0.05), and with the increase of YEP concentration, the body weight growth rate showed a trend of decline. The content of TG in hemolymph of silkworms after feeding with different concentrations of YEP for 5 days was 1.42 to 1.98 times higher than that of control group, and the differences between the control group and the groups JM1 or JM2 were significant (P<0.05). The content of UA in hemolymph of silkworms of each group showed a trend of decline with the extension of time. Compared with the 3rd day, the content of UA in hemolymph of silkworms of each group was significantly decreased compared with the 5th day (P<0.05). The content of UA in hemolymph of silkworms of each time point showed a trend of increase with the increase of YEP concentration, but the differences were not significant between the control group and the experimental groups (P>0.05). The activity of XOD in hemolymph of silkworms of each time point was significantly higher than that of control group (P<0.05), but it was firstly increased and then decreased with the increase of YEP concentration. There was a significant or an extremely significant correlation between the content of UA and the activity of XOD in hemolymph of silkworms (P<0.05 or P<0.01), and the correlation coefficient ranged from 0.652 to 0.902. It is concluded that feeding a certain concentration of YEP can lead to increases of UA content, TG content and XOD activity in hemolymph, thus affecting the UA metabolism of silkworms.

Cite this article

LI Na, JIN Huijie, JIA Manli, HUANG Lu, XIE Yan, XIA Aihua, YANG Guiming, LI Jisheng . Effects of Yeast Extract Powder on Triglyceride Content and Uric Acid Metabolism in Hemolymph of Silkworms[J]. Chinese Journal of Animal Nutrition, 2018 , 30(6) : 2245 -2251 . DOI: 10.3969/j.issn.1006-267x.2018.06.028

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