Molecular Nutrition

Effects of Lactobacillus plantarum on Morphological, Survival and Immunity Response of Escherichia coli Infected Intestinal Epithelial Cells

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  • Institute of Animal Husbandry and Veterinary Medicine, Beijing 100097, China

Received date: 2017-11-20

  Online published: 2018-06-20

Abstract

The aim of this study was to investigate the effects of Lactobacillus plantarum on morphological, survival and immunity response of Escherichia coli infected intestinal epithelial cells (IPEC-J2 cells). Under the conditions of in vitro, the effects of Lactobacillus plantarum on morphological and survival of Escherichia coli infected IPEC-J2 cells were assessed using giemsa staining and trypan blue staining methods, and the effects of Lactobacillus plantarum on immunity response of Escherichia coli infected IPEC-J2 cells were assessed using real-time PCR method. The results showed that Lactobacillus plantarum could ameliorate Escherichia coli-induced disruption of cell morphology on IPEC-J2 cells. Compared with the Escherichia coli treated group, the perecentage of dead cells was significantly decreased by Lactobacillus plantarum and Escherichia coli treated (1, 2 h, P<0.01; 3 h, P<0.05). The Lactobacillus plantarum could significantly inhibit the mRNA overexpressions of Toll-like receptor 2 (TLR2) (2, 3 h,P<0.01), Toll-like receptor 6 (TLR6) (2 h,P<0.05;3 h,P<0.01), NOD-like receptor 2 (NOD2) (1 h,P<0.05;2 h,P<0.01) and inflammatory cytokines interleukin-6 (IL-6) (2, 3 h,P<0.01), interleukin-8 (IL-8) (2, 3 h,P<0.01) in IPEC-J2 cells induced by Escherichia coli, and could promote the NOD-like receptor 1 (NOD1) mRNA expression in IPEC-J2 cells (2 h,P<0.05;3 h,P<0.01). The results indicated that Lactobacillus plantarum can ameliorate Escherichia coli-induced the disruption of cell morphology and death of IPEC-J2 cells. In addition, it can suppress the Escherichia coli-induced mRNA overexpressions of inflammatory cytokines IL-6 and IL-8 by regulating the pattern recognition receptor mRNA expression of TLR2, TLR6 and NOD2.

Cite this article

ZENG Yanxia, WANG Sixin, ZHANG Wei, LIU Hui, ZHANG Dongyan, WANG Yamin, JI Haifeng, WANG Jing . Effects of Lactobacillus plantarum on Morphological, Survival and Immunity Response of Escherichia coli Infected Intestinal Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2018 , 30(6) : 2262 -2270 . DOI: 10.3969/j.issn.1006-267x.2018.06.030

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