Effects of Different Sources of Xylanases and Their Combination on Intestinal Morphology, Disaccharidase Activities and Gene Expression of Broilers

HUANG Jingxi, LI Zhongliang, ZANG Xupeng, YU Xiaohua, ZHANG Tao, ZUO Jianjun, FENG Dingyuan

doi:10.3969/j.issn.1006-267x.2018.06.031

2018 , Vol. 30 >Issue 6: 2271 - 2280

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.06.031

Molecular Nutrition

Effects of Different Sources of Xylanases and Their Combination on Intestinal Morphology, Disaccharidase Activities and Gene Expression of Broilers

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1. Animal Science College of South China Agriculture University, Guangzhou 510642, China;
2. Evonik Degussa(China) Co., Ltd., Beijing 100600, China

Received date: 2017-11-20

Online published: 2018-06-20

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Abstract

This experiment was conducted to investigate the effects of different sources of xylanases and their combination on intestinal morphology, disaccharidase activities and gene expression of broilers. A total of 1 560 one-day-old healthy yellow-feather male broilers were randomly divided into 4 groups with 6 replicates per group and 65 broilers per replicate. Broilers in control group (group Ⅰ) were fed a low energy and protein wheat-soybean type basal diet. Broilers in experiment groups (groups Ⅱ, Ⅲ and Ⅳ) were fed the diets supplemented with 4 000 U/kg of xylanase 1^# from Aspergillus, xylanase 2^# from Trichoderma and the combined xylanase (xylanase 1^#:xylanase 2^#=1:1) based on the basal diet, respectively. The experiment period was 63 days. Intestinal tissues were harvested from two broilers of each replicate at 21, 42 and 63 days of age for the determination of mucosal morphology, mucosal disaccharidase activities and disaccharidase gene mRNA expression abundances. The results showed as follows:1) the crypt depth of group Ⅳ in jejunum at 21 days of age was significantly lower than that of group Ⅰ (P<0.05), while the villus height/crypt depth (V/C) value of group Ⅳ in duodenum at 42 days of age was significantly greater than that of group Ⅰ (P<0.01) and groups Ⅱ and Ⅲ (P<0.05), and V/C value of group Ⅳ in jejunum at 42 days of age was significantly higher than that of groups Ⅰ, Ⅱ and Ⅲ (P<0.01). 2) At 21 days of age, the activity of maltase in duodenum and jejunum and the activity of sucrase in jejunum of group Ⅳ were significantly higher than those of groups Ⅰ, Ⅱ and Ⅲ (P<0.01); at 42 days of age, the activity of sucrase in jejunum of group Ⅳ was significantly higher than that of groups Ⅰand Ⅱ (P<0.01) and group Ⅲ (P<0.05). 3) At 42 days of age, the expression abundance of maltase-glucoamylase (MGA) mRNA in jejunum of group Ⅳ was significantly higher than that of groups Ⅰ and Ⅱ (P<0.01). Thus, the supplementation of xylanase can improve intestinal mucosal morphology and promote the secretion of disaccharidases and their gene expression during broilers in the early (1 to 21 days of age) and middle (22 to 42 days of age) stages. And the effect of combined xylanase is better than the single xylanase, showing a combination of efficient catalytic effect.

Key words： broilers; combined xylanase; mucosal morphology; disaccharidase; gene expression

Cite this article

HUANG Jingxi, LI Zhongliang, ZANG Xupeng, YU Xiaohua, ZHANG Tao, ZUO Jianjun, FENG Dingyuan . Effects of Different Sources of Xylanases and Their Combination on Intestinal Morphology, Disaccharidase Activities and Gene Expression of Broilers[J]. Chinese Journal of Animal Nutrition, 2018 , 30(6) : 2271 -2280 . DOI: 10.3969/j.issn.1006-267x.2018.06.031

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