Comparative Study on Immunomodulatory Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharide of Mice

YANG Tianren, WANG Shuai, HUANG Shuo, SHANG Lijun, YU Haitao, ZENG Xiangfang, QIAO Shiyan

doi:10.3969/j.issn.1006-267x.2018.06.038

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 6: 2337 - 2345

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.06.038

Molecular Nutrition

Comparative Study on Immunomodulatory Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharide of Mice

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1. Beijing Key Laboratory of Biological Feed Additive, China Agricultural University, Beijing 100193, China;
2. College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2017-12-01

Online published: 2018-06-20

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Abstract

This experiment was conducted to compare the regulating effect of antimicrobial peptide Sublancin and Astragalus polysaccharide on immune function of mice. Sixty female BALB/c mice at the age of 4 to 6 weeks were randomly assigned into 6 groups with 10 mice per group. Mice in blank control group received saline by intragastric administration, mice in challenged group received saline by intragastric administration, mice in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups received 12.0 and 48.0 mg/kg BW Astragalus polysaccharide solution by intragastric administration, respectively, and mice in 1.0 and 2.0 mg/kg BW Sublancin groups received 1.0 and 2.0 mg/kg BW Sublancin solution by intragastric administration, respectively. All the mice were administrated orally daily for 7 days, and the dose of each mouse was 0.2 mL. Except blank control group, mice in other 5 groups were challenged with Salmonella typhimurium (1×10⁹ CFU/mL, 200 μL per mouse) on day 8 (24 h after intragastric administration). Five mice from each group were randomly selected 3 and 24 h after challenge and the peripheral blood, spleen and caecum contents were sampled to measure the serum cytokine contents, T lymphocyte subsets CD₄⁺ and CD₈⁺ numbers in splenocyte and Salmonella number in intestinal contents, and so on. The results showed as follows:challenged 3 h by Salmonella, compared with the challenged group, the serum interleukin-6 (IL-6) content in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups and 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05); the serum interleukin-10 (IL-10) content in 1.0 mg/kg BW Sublancin group was significantly increased (P<0.05); the serum tumor necrosis factor-α (TNF-α) content in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups, 1.0 and 2.0 mg/kg BW Sublancin groups was significantly reduced (P<0.05), but the serum interleukin-25 (IL-25) content was not significantly different (P>0.05); the serum monocyte chemoattractant protein-1 (MCP-1) content in 48.0 mg/kg BW Astragalus polysaccharide group and 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05); CD₄⁺/CD₈⁺ in splenocyte in 48.0 mg/kg BW Astragalus polysaccharide group, 1.0 and 2.0 mg/kg BW Sublancin groups was significantly increased (P<0.05); the Salmonella number in intestinal contents in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups, 1.0 and 2.0 mg/kg BW Sublancin groups had no significant changes (P>0.05), but had reducing trend. Challenged 24 h by Salmonella, compared with the challenged group, the serum IL-6 content in 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05); the serum TNF-α content in 48.0 mg/kg BW Astragalus polysaccharide group and 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05); the serum MCP-1 content in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups, 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05). Moreover, the serum IL-10 content in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups, 1.0 and 2.0 mg/kg BW Sublancin groups was significantly increased challenged 24 h by Salmonella compared with blank control group (P<0.05). These results indicate that antimicrobial peptide Sublancin and Astragalus polysaccharide have regulating effect on immune function for mice, and the immunomodulatory effect of antimicrobial peptide Sublancin is more comprehensive than that of Astragalus polysaccharide.

Key words： antimicrobial peptide; Sublancin; Astragalus polysaccharide; mice; immune function

Cite this article

YANG Tianren, WANG Shuai, HUANG Shuo, SHANG Lijun, YU Haitao, ZENG Xiangfang, QIAO Shiyan . Comparative Study on Immunomodulatory Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharide of Mice[J]. Chinese Journal of Animal Nutrition, 2018 , 30(6) : 2337 -2345 . DOI: 10.3969/j.issn.1006-267x.2018.06.038

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