Research in Extraction Process and Antioxidant Activity of Total Tannin from Abutilon theophrasti Medic. Leaves

YANG Jie, CHEN Xiaoyun, GUO Yuru, GAO Xiang, TIAN Chunlian, LIU Mingchun

doi:10.3969/j.issn.1006-267x.2018.06.043

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 6: 2377 - 2384

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.06.043

Molecular Nutrition

Research in Extraction Process and Antioxidant Activity of Total Tannin from Abutilon theophrasti Medic. Leaves

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College of Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China

Received date: 2017-11-28

Online published: 2018-06-20

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Abstract

In order to investigate extraction process and antioxidant activity of total tannins from Abutilon theophrasti Medic. leaves, single factor experiments and response surface experiments were established to optimize the extraction process of total tannins from Abutilon theophrasti Medic. leaves, and antioxidant activities of total polyphenols, non-absorbed polyphenols and total tannins were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS)free radical scavenging assay and Ferric ion reducing antioxidant power (FRAP) analysis. The results showed that the optimal extraction conditions were as follows:acetone concentration was 70%, the ratio of solvent to material was 15.25 mL/g and the extraction time was 20 min. Under the above conditions, the yield of total tannins from Abutilon theophrasti Medic. leaves collected in August, September, October was (1.41±0.05)‰, (1.77±0.03)‰ and (1.49±0.03)‰, respectively. Total tannins extracted from Abutilon theophrasti Medic. leaves showed good antioxidant activity. In conclusion, extraction processes with high extraction rate for Abutilon theophrasti Medic. leaves are obtained in the present study, and the extracted total tannins have high antioxidant activity.

Key words： Abutilon theophrasti Medic. leaves; total tannins; extraction technology; response surface; antioxidant activity

Cite this article

YANG Jie, CHEN Xiaoyun, GUO Yuru, GAO Xiang, TIAN Chunlian, LIU Mingchun . Research in Extraction Process and Antioxidant Activity of Total Tannin from Abutilon theophrasti Medic. Leaves[J]. Chinese Journal of Animal Nutrition, 2018 , 30(6) : 2377 -2384 . DOI: 10.3969/j.issn.1006-267x.2018.06.043

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