Molecular Nutrition

Effects of Dietary Epidermal Growth Factor on Serum Biochemical Indices, Serum Free Amino Acid and Intestinal Mucosal Hydrolytic Amino Acid Contents of Weaned Piglets

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  • 1. Animal Medical School of Hunan Agricultural University, Changsha 410128, China;
    2. Key Laboratory of Subtropical Agricultural Ecological Process of Chinese Academy of Sciences, Sub-Tropical Agricultural Ecology Research Institute of Chinese Academy of Sciences, Changsha 410125, China;
    3. Animal Nutrition And Human Health Laboratory, Life Sciences College, Hunan Normal University, Changsha 410081, China

Received date: 2018-01-03

  Online published: 2018-07-20

Abstract

The objective of this experiment was to investigate the effects of dietary epidermal growth factor (EGF) on serum biochemical index, serum free amino acid and intestinal mucosal hydrolytic amino acid contents of weaned piglets. Forty-two healthy piglets with similar body weight were weaned at 21 days of age and randomly assigned into 3 groups with 14 replicates per group and 1 piglet per replicate. Piglets in group Ⅰ (control group) were fed a basal diet, and the others in groups Ⅱ and Ⅲ were fed the basal diet supplemented with 200 and 400 μg/kg EGF, respectively. The experiment lasted for 14 days. The results showed as follows:1) dietary supplemented with EGF had no significant effects on average daily gain, average daily feed intake and feed to gain ratio of weaned piglets (P>0.05). 2) After weaning 7 days, the serum uric acid content in group Ⅱ was significantly higher than that in group Ⅰ (P<0.05), the serum immunoglobulin A content in groups Ⅱ and Ⅲ was significantly lower than that in group Ⅰ (P<0.05). After weaning 14 days, the serum creatinine content in group Ⅲ was significantly lower than that in group Ⅰ (P<0.05), the serum glutamic-oxalacetic transaminase/glutamic-pyruvic transaminase value in group Ⅲ was significantly lower than that in groups Ⅰ and Ⅱ (P<0.05). Dietary supplemented with EGF had no significant effects on serum urea nitrogen, glucose, total protein, albumin, globulin, immunoglobulin M, immunoglobulin G, insulin-like growth factor-1, growth hormone contents, and albumin/globulin and glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase activities of weaned piglets (P>0.05). 3) After weaning 7 days, the contents of taurine, alanine, citrullinine and histidine in serum in group Ⅱ were significantly lower than those in group Ⅲ (P<0.05), the contents of arginine, glutamate and serine in serum in group Ⅱ were significantly lower than those in groups Ⅰ and Ⅲ (P<0.05), and there were no significant differences on other serum amino acid contents among all groups (P>0.05). 4) After weaning 14 days, compared with groups Ⅰand Ⅲ, the contents of valine in jejunum mucosa and cysteine in ileum mucosa in group Ⅱ were significantly increased (P<0.05), and the contents of serine, glutamic acid, alanine, cysteine, isoleucine, phenylalanine and histidine in jejunum mucosa in group Ⅱ were tended to be decreased (P<0.10). In conclusion, dietary supplemented with high dose EGF can reduce serum immunoglobulin A and other biochemical indices contents, while dietary supplemented with low dose EGF can reduce the contents of some amino acid in serum and intestinal mucosa.

Cite this article

ZHU Fan, WANG Lixia, JIA Xinglin, YIN Jia, YANG Huansheng, YIN Yulong . Effects of Dietary Epidermal Growth Factor on Serum Biochemical Indices, Serum Free Amino Acid and Intestinal Mucosal Hydrolytic Amino Acid Contents of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2018 , 30(7) : 2519 -2528 . DOI: 10.3969/j.issn.1006-267x.2018.07.012

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