Effects of Vitamin A on Growth, Metabolism, Antioxidant Capacity and Immunity of Juvenile Black Carp (Mylopharyngodon piceus)

CHEN Lian, WU Chenglong, ZHANG Yixiang, SHAO Xianping, CHEN Shujian, WANG Chunlin, YE Jinyun

doi:10.3969/j.issn.1006-267x.2018.07.019

2018 , Vol. 30 >Issue 7: 2594 - 2605

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.07.019

Molecular Nutrition

Effects of Vitamin A on Growth, Metabolism, Antioxidant Capacity and Immunity of Juvenile Black Carp (Mylopharyngodon piceus)

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1. Key Laboratory of Applied Marine Biotechnology of Education, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, School of Life Science, Huzhou University, Huzhou 313000, China

Received date: 2018-01-18

Online published: 2018-07-20

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Abstract

The present trial was conducted to determine the effects of diets with six vitamin A contents (290, 1 033, 1 734, 3 835, 7 662 and 14 943 IU/kg) on growth, metabolism, antioxidant capacity and immunity of juvenile black carp (Mylopharyngodon piceus). A total of 540 juvenile black carps with initial body weight of (5.35±0.16) g were divided into six groups, and carps in each were fed one of the six diets. Each group had 3 replicates with 30 carps per replicate. The experiment lasted for 60 days. The results showed as follows:with the increase of dietary vitamin A content, weight gain rate (WGR), specific growth rate (SGR) and protein efficiency ratio (PER) firstly increased and then decreased, and reached to the highest when dietary vitamin A was 3 835 IU/kg. Based on the broken-line regression analysis for WGR, the optimal content of dietary vitamin A was 2 246.64 IU/kg for the maximal WGR. 2) When dietary vitamin A content was increased from 290 to 7 662 IU/kg, the activities of α amylase, trypsin, chymotrypsin, elastase in liver were significantly increased (P<0.05), similarly, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), and total antioxidant capacities (T-AOC) in liver were also significantly up-regulated (P<0.05). The activities of glutathione-S-transferase (GST) and content of malondialdehyde (MDA) were firstly decreased and reached to the lowest level when dietary vitamin A was 3 835 IU/kg, and then increased. 3) Compared with 290 IU/kg group, the activity of lysozyme (LYZ) and contents of complement 3 (C3) and complement 4(C4) in serum of in 3 835 and 7 662 IU/kg groups were significantly increased (P<0.05). Proper contents of dietary vitamin A (1 734, 3 835 and 7 662 IU/kg) could also significantly up-regulate the gene expression levels of LYZ, interferon α (IFNα), hepcidin (HEPC), natural resistance-associated macrophage protein (NRAMP), C3 and complement 9 (C9) in blood cell (P<0.05). Therefore, adequate dietary vitamin A can significantly increase growth performances, improve metabolic abilities and then enhance non-specific immunity in juvenile black carp; according to the WGR, the optimal vitamin A content in diets for juvenile black carp is 2 246.64 IU/kg.

Key words： vitamin A; juvenile black carp (Mylopharyngodon piceus); requirement; antioxidant capacity; immune response

Cite this article

CHEN Lian, WU Chenglong, ZHANG Yixiang, SHAO Xianping, CHEN Shujian, WANG Chunlin, YE Jinyun . Effects of Vitamin A on Growth, Metabolism, Antioxidant Capacity and Immunity of Juvenile Black Carp (Mylopharyngodon piceus)[J]. Chinese Journal of Animal Nutrition, 2018 , 30(7) : 2594 -2605 . DOI: 10.3969/j.issn.1006-267x.2018.07.019

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