Molecular Nutrition

Distribution and Expression of Heat Shock Protein 70 in Stomachs of Yaks

Expand
  • College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2017-12-19

  Online published: 2018-07-20

Abstract

The purpose of this study was to study the distribution characteristics and the differential changes of expression level of heat shock protein 70 (HSP70) in stomachs of yaks. The tissues of stomachs of 8 adult healthy yaks were collected, and the type of cells in the abomasal gland was studied by histological staining. The distribution and expression of HSP70 in different stomachs were detected by the methods of immunohistochemistry and Western blotting, respectively. The results showed as follows:Gordon sweet staining and Alcian blue-periodic acid-Schiff (AB-PAS) staining showed that a large number of endocrine cells were found in the abomasal gland. Immunohistochemistry analysis showed that in rumen, reticulum and omasum, HSP70 only positively expressed in the epithelial cell layer, strongly positively expressed in stratum corneum cells, some cells in basal layer, granular layer and spinous layer were moderately positive expressed, whereas some other cells were negatively expressed. The scope of the abomasum positive reaction was very wide, not only expressed in the epithelial cells of mucous surface, but also expressed in the endocrine cells and parietal cells in the abomasal gland. Meanwhile, the positive reaction was mainly concentrated in the cytoplasm and nucleus. Immunohistochemistry and Western blotting results both showed that the highest expression level of HSP70 was found in the abomasal gland of yaks, the expression level in rumen, reticulum and omasum was descended in order, and the differences among parts were significant (P<0.01). In conclusion, a large number of argyrophilia endocrine cells are found in the abomasal gland of yaks. HSP70 is distributed in all stomachs of yaks, and the expression of the abomasum is obviously higher than that of other stomachs, which suggests that HSP70 may be associated with digestion and absorption of yaks' stomach.

Cite this article

YANG Xiaoqing, CUI Yan, HE Junfeng, YANG Xue, LIU Penggang, LIAO Bo, LI Hui, SUN Juan . Distribution and Expression of Heat Shock Protein 70 in Stomachs of Yaks[J]. Chinese Journal of Animal Nutrition, 2018 , 30(7) : 2626 -2631 . DOI: 10.3969/j.issn.1006-267x.2018.07.022

References

[1] GEHRMANN M,CERVELLO M,MONTALTO G,et al.Heat shock protein 70 serum levels differ significantly in patients with chronic hepatitis,liver cirrhosis,and hepatocellular Carcinoma[J].Frontiers in Immunology,2014,5:307.

[2] LARGE A T,GOLDBERG M D,LUND P A.Chaperones and protein folding in the archaea[J].Biochemical Society Transactions,2009,37(1):46-51.  

[3] MUSTAFI S B,CHAKRABORTY P K,DEY R S,et al.Heat stress upregulates chaperone heat shock protein 70 and antioxidant manganese superoxide dismutase through reactive oxygen species (ROS),p38MAPK,and Akt[J].Cell Stress and Chaperones,2009,14(6):579-589.  

[4] ALWAJEEH N S,HAJEREZAIE M,NOOR S M,et al.The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats[J].BMC Veterinary Research,2017,13(1):27.

[5] OMAR H,NORDIN N,HASSANDARVISH P,et al.Methanol leaf extract of Actinodaphne sesquipedalis (Lauraceae) enhances gastric defense against ethanol-induced ulcer in rats[J].Drug Design,Development and Therapy,2017,11:1353-1365.

[6] MARRUCHELLA G,LEONARDO M D,GUARDO G D,et al.Heat shock proteins (HSPs) 27,72 and 73 in normal and pre-ulcerative mucosa of the gastric pars oesophagea in swine[J].Journal of Comparative Pathology,2004,131(1):10-17.  

[7] DA SILVA M S,DE ALMEIDA A C A,DE FARIA F M,et al.Abarema cochliacarpos:gastroprotective and ulcer-healing activities[J].Journal of Ethnopharmacology,2010,132(1):134-142.  

[8] JIANG M,CHEN Y,WANG Y,et al.Yak (Bos grunniens) stomach lysozyme:molecular cloning,expression and its antibacterial activities[J].Animal Biotechnology,2010,21(1):25-35.

[9] OHKAWARA T,NISHIHIRA J,TAKEDA H,et al.Protective effect of geranylgeranylacetone on trinitrobenzene sulfonic acid-induced colitis in mice[J].International Journal of Molecular Medicine,2006,17(2):229-234.

[10] 徐革锋,刘洋,牟振波.鱼类消化道内分泌细胞概述及研究方法[J].水产学杂志,2011,24(03):60-64,68.

[11] 王雯慧,陈怀涛.双峰驼胃底腺区黏膜组织学与组织化学研究[J].畜牧兽医学报,2003(04):372-377.

[12] ASSIMAKOPOULOS S F,SCOPA C D,ZERVOUDAKIS G,et al.Bombesin and neurotensin reduce endotoxemia,intestinal oxidative stress,and apoptosis in experimental obstructive jaundice[J].Annals of Surgery,2005,241(1):159-167.

[13] LAINE L,TAKEUCHI K,TARNAWSKI A.Gastric mucosal defense and cytoprotection:bench to bedside[J].Gastroenterology,2008,135(1):41.

[14] 赵为红,寿好长,闫福岭.细胞凋亡[M].郑州:河南医科大学出版社,1998.

[15] DONNELLY B F,NEEDHAM P G,SNYDER A C,et al.Hsp70 and Hsp90 multichaperone complexes sequentially regulate thiazide-sensitive cotransporter endoplasmic reticulum-associated degradation and biogenesis[J].Journal of Biological Chemistry,2013,288(18):13124-13135.  

[16] SAIKA M,UEYAMA T,SENBA E.Expression of immediate early genes,HSP70,and COX-2 mRNAs in rat stomach following ethanol ingestion[J].Digestive Diseases and Sciences,2000,45(12):2455-2462.  

[17] OTAKA M,ODASHIMA M,IZUMI Y,et al.Target molecules of molecular chaperone (HSP70 family) in injured gastric mucosa in vivo[J].Life Sciences,2009,84(19/20):664-667.

[18] 张绍敏,邹湉,付小义,陈出新.热休克蛋白70与慢性糜烂性胃炎胃黏膜损伤的相关性分析及其临床意义[J].国际消化病杂志,2016,36(05):291-294.

[19] 冯丹,欧阳佳良,王梦芝,等.饲粮中不同比例桑叶粉对湖羊瘤胃上皮组织结构的影响[J].动物营养学报,2017,29(6):2196-2203.

[20] DAS NEVES CARDOSO N,DA SILVEIRA FIRMIANO E M,GOMES I D,et al.Histochemical and immunohistochemical study on endocrine cells (5HT,GAS,and SST) of the gastrointestinal tract of a teleost,the characin Astyanax bimaculatus[J].Acta Histochemica,2015,117(7):595-604.  

[21] LINDSTRÖM E,CHEN D,NORLÉN P,et al.Control of gastric acid secretion:the gastrin-ECL cell-parietal cell axis[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2001,128(3):503-511.  

[22] WADA I,OTAKA M,JIN M,et al.Expression of HSP72 in the gastric mucosa is regulated by gastric acid in rats-correlation of HSP72 expression with mucosal protection[J].Biochemical and Biophysical Research Communications,2006,349(2):611-618.  

[23] ISHIHARA T,SUEMASU S,ASANO T,et al.Stimulation of gastric ulcer healing by heat shock protein 70[J].Biochemical Pharmacology,2011,82(7):728-736.  

[24] TSUKIMI Y,OKABE S.Recent advances in gastrointestinal pathophysiology:role of heat shock proteins in mucosal defense and ulcer healing[J].Biological & Pharmaceutical Bulletin,2001,24(1):1-9.  

[25] ARORA N,ALSAIED O,DAUER P,et al.Downregulation of Sp1 by Minnelide leads to decrease in HSP70 and decrease in tumor burden of gastric cancer[J].PLoS One,2017,12(2):e0171827.
Outlines

/