Effects of Chitooligosaccharides on Growth Performance, Antioxidant Capacity and Nutrient Digestion and Transport Capacity of Jejunum of Oxidative Stress Piglets

TIAN Gang, HUANG Linhui, SONG Xiaohua, CHEN Daiwen, MAO Xiangbing, YU Jie, ZHENG Ping, HE Jun, YU Bing

doi:10.3969/j.issn.1006-267x.2018.07.025

2018 , Vol. 30 >Issue 7: 2652 - 2661

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.07.025

Molecular Nutrition

Effects of Chitooligosaccharides on Growth Performance, Antioxidant Capacity and Nutrient Digestion and Transport Capacity of Jejunum of Oxidative Stress Piglets

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1. Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2017-12-13

Online published: 2018-07-20

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Abstract

This study was conducted to evaluate the effects of dietary chitooligosaccharides (COS) on growth performance, antioxidant capacity and nutrient digestion and transport capacity of jejunum of piglets under normal feeding and oxidative stress statues. Twenty four 24-day-old healthy Duroc×Landrace×Yorkshire weaned piglets with average body weight (BW) of (7.34±0.09) kg were randomly divided into control, COS, diquat, COS+diquat groups with 6 replicates per group and 1 pig per replicate by a 2×2 two factorial arrangement. The trial lasted for 28 days. COS was given all in the trial (50 mg/kg diet). Diquat challenged pigs were given a single intraperitoneal injection of 10 mg/kg BW diquat at the 22^nd day and non-challenged pigs were injected intraperitoneally with the equal amount of physiological saline at the same time. Digestion trial was carried out from the 18^th to 21^st day by the method of endogenous indicator, blood were fasting sampled via anterior vena cava and then pigs were injected with diquat in the morning of the 22^nd day, and pigs were slaughtered after blood sampled via anterior vena cava and jejunum mucosa samples were sampled in the morning of the 29^th day. The results showed as follows:1) before diquat injection, there were no significant effects of dietary COS on average daily feed intake (ADFI) and average daily gain (ADG) of piglets (P>0.05), but the ratio of feed to gain (F/G) was tended to decreased (P=0.09). Dietary COS significantly increased the apparent digestibilities of dry matter, organism matter, crude protein, ether extract, total energy, ash, calcium and phosphor in diet of piglets (P<0.05), and significantly increased the activity of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in plasma of piglets (P<0.05). 2) After diquat injection, ADG and ADFI of piglets were significantly decreased and F/G was significantly increased (P<0.01). Dietary COS significantly inhibited the reduction of ADG of piglets induced by diquat injection (P<0.05). 3) After diquat injection, the activity of catalase (CAT) in plasma (P<0.01) and lactase, sucrase and maltase of jejunum mucosa (P<0.05), and mRNA expression levels of glucose transporter 2 (GLUT2) and sodium/glucose transporter 1 (SGLT1) of jejunum mucosa (P<0.05) of piglets were significantly decreased. Dietary COS significantly increased the activity of SOD and T-AOC in plasma (P<0.05), and significantly relieved reduction of the activities of disaccharidases and mRNA expression levels of GLUT2 and SGLT1 of jejunum mucosa of oxidative stress piglets (P<0.05). In conclusion, under normal feeding statues, dietary 50 mg/kg COS can significantly improve nutrients digestibilities in diets and antioxidant capacity, decrease F/G of piglets. In oxidative stress statues, COS can improve antioxidant capacity, alleviate oxidative stress induced by diquat injection, improve nutrient digestion and transport capacity of jejunum and relieve the reduction of weight gain of oxidative stress piglets.

Key words： chitooligosaccharides; weaned piglets; antioxidant; jejunum mucosa; nutrient digestion and transport

Cite this article

TIAN Gang, HUANG Linhui, SONG Xiaohua, CHEN Daiwen, MAO Xiangbing, YU Jie, ZHENG Ping, HE Jun, YU Bing . Effects of Chitooligosaccharides on Growth Performance, Antioxidant Capacity and Nutrient Digestion and Transport Capacity of Jejunum of Oxidative Stress Piglets[J]. Chinese Journal of Animal Nutrition, 2018 , 30(7) : 2652 -2661 . DOI: 10.3969/j.issn.1006-267x.2018.07.025

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