Molecular Nutrition

Effects of Nisin on Caecal Microflora Structure and Lipid Metabolism of Mice with Diarrhea

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  • 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. College of Veterinary, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2017-12-04

  Online published: 2018-07-20

Abstract

The aim of this study was to investigate the effects of Nisin on cecal microflora structure and lipid metabolism of mice with diarrhea. Fifty 7 to 9 weeks old specific pathogen free (SPF) mice (half male and half female) were randomly divided into five groups:blank control group, negative control group, ciprofloxacin group, ampicillin group and Nisin group. The mice in negative control group, ciprofloxacin group, ampicillin group and Nisin group were intraperitoneally injected with pathogenic Escherichia coli suspension (2.50×1011 CFU/mL) at the dose of 0.2 mL per mouse for successive 3 days to establish the diarrheal model, and the mice in blank control group were intraperitoneally injected with equivalent sterrilled normal saline. After injection 3 days, the mice in blank control group and negative control group were given sterrilled normal saline by gavage, and the mice in other groups were given correspondent substances by gavage for 15 days, the dose of 0.3 mL each time, twice times a day. Samples were collected after 2 h administration on day15, the microflora structure of caecum content was analyzed by high throughput sequencing technology, and the contents of a disintegrin and metalloproteinase with thrombospondin (ADAMTS1), total cholesterol (TC) and insulin (INS) in serum, jejunum, ileum and brain tissue were measured by enzyme-linked immunosorbent assay (ELISA) method. The results showed as follows:compared with the negative control group, Nisin could significantly decrease the ADAMTS1 content in serum, ileum and brain tissue and the INS content in ileum (P<0.05), significantly increase the TC content in serum, jejunum and brain tissue (P<0.05), and significantly increase the body weight of mice in each stage (P<0.05). The caecal microflora richness (ACE index=2 469.54, Chao1 index=3 340.29) and diversity (Shannon index=7.56) of mice in Nisin group were the highest, and those in negative control group were the lowest. Verrucomicrobia, Cyanobacteria, Bacteroidetes and Firmicutes were dominant phylums in Nisin group, and Actinobacteria, Gemmatimonadetes and Proteobacteria were dominant phylums in negative control group. The results indicate that Nisin can enhance the diversity of caecal microflora, decrease the decrease the ADAMTS1 content in serum, ileum and brain tissue and the INS content in ileum, increase the TC content in serum, jejunum and brain tissue, and then affect the cecal microflora structure and lipid metabolism of mice with diarrhea.

Cite this article

HE Meiling, WANG Chunjie, JIA Zhifeng, Simujide, Jimisi, LIU Bo, CHEN Hao, CHAO Ke, Aorigele . Effects of Nisin on Caecal Microflora Structure and Lipid Metabolism of Mice with Diarrhea[J]. Chinese Journal of Animal Nutrition, 2018 , 30(7) : 2782 -2790 . DOI: 10.3969/j.issn.1006-267x.2018.07.039

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