Effects of Lactobacillus delbrueckii on Immune and Antioxidant Indexes in Skin Mucous and Antibacterial Peptide Gene Expression in Skin of Cyprinus carpio Huanghe var.

ZENG Qinghui, LIU Min, YAO Yuhong, DING Xiangfei, SHI Lisha, LI Xiaofan, ZHANG Chunnuan

doi:10.3969/j.issn.1006-267x.2018.07.050

2018 , Vol. 30 >Issue 7: 2872 - 2878

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.07.050

Molecular Nutrition

Effects of Lactobacillus delbrueckii on Immune and Antioxidant Indexes in Skin Mucous and Antibacterial Peptide Gene Expression in Skin of Cyprinus carpio Huanghe var.

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College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, China

Received date: 2018-01-05

Online published: 2018-07-20

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Abstract

The aim of the present study was to investigate the effects of Lactobacillus delbrueckii (L. delbrueckii) on the immune and antioxidant indexes in skin mucous and the antimicrobial peptide gene expression in skin of Cyprinus carpio Huanghe var. A total of 450 Cyprinus carpio Huanghe var. with the initial body weight of (15.0±0.5) g were randomly distributed into five groups with three replicates per group and 30 fish per replicate. The fish in those five groups fed diets containing 0 (control group), 1×10⁵, 1×10⁶, 1×10⁷ and 1×10⁸ CFU/g L. delbrueckii, respectively, for 8 weeks. At the end of feeding trail, the skin mucous and skin were collected, and the immune and antioxidant indexes in the skin mucous and the relative expression levels of antimicrobial peptide genes including liver-expressed antimicrobial peptide-1 (Leap-1) and liver-expressed antimicrobial peptide-2 (Leap-2) in the skin were determined. The results showed that the activities of lysozyme (LYZ), alkaline phosphatase (AKP), superoxide dismutase (SOD) and the contents of complement 3 (C3) and complement 4 (C4) in skin mucous of the 1×10⁶ and 1×10⁷ CFU/g L. delbrueckii groups were significantly higher than those of the control group (P<0.05), while the malondialdehyde (MDA) content in skin mucous of the 1×10⁶ and 1×10⁷ CFU/g L. delbrueckii groups was significantly lower than that of the control group (P<0.05). There were no significant differences of above parameters between the 1×10⁵ or 1×10⁸ CFU/g L. delbrueckii groups and the control group (P>0.05). No significant difference was found in the acid phosphatase (ACP) activity in skin mucous among groups (P>0.05). The activities of catalase (CAT) and glutathion peroxidase (GPx) in skin mucous of the 1×10⁶ CFU/g L. delbrueckii group were significantly higher than those of the control group (P<0.05), and the total antioxidant capacity (T-AOC) in skin mucous of the 1×10⁵, 1×10⁶ and 1×10⁷ CFU/g L. delbrueckii groups was significantly higher than that of the control group (P<0.05). In addition, the relative expression levels of antimicrobial peptide genes Leap-1 and Leap-2 in skin of all experimental groups were significantly up-regulated compared with the control group (P<0.05), and highest values were all found in the 1×10⁷ CFU/g L. delbrueckii group. Those results demonstrate that diet supplemented with 1×10⁶ to 1×10⁷ CFU/g L. delbrueckii can enhance the immunity, antioxidant capability and Leap-1 and Leap-2 gene expression in skin of Cyprinus carpio Huanghe var.

Key words： Lactobacillus delbrueckii; Cyprinus carpio Huanghe var.; skin; immune; antioxidant

Cite this article

ZENG Qinghui, LIU Min, YAO Yuhong, DING Xiangfei, SHI Lisha, LI Xiaofan, ZHANG Chunnuan . Effects of Lactobacillus delbrueckii on Immune and Antioxidant Indexes in Skin Mucous and Antibacterial Peptide Gene Expression in Skin of Cyprinus carpio Huanghe var.[J]. Chinese Journal of Animal Nutrition, 2018 , 30(7) : 2872 -2878 . DOI: 10.3969/j.issn.1006-267x.2018.07.050

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