Effects of Weaning Days of Age on Ruminal Microbial Diversity in Hu Lambs

MA Wanhao, ZHANG Ning, LI Fei, LI Fadi

doi:10.3969/j.issn.1006-267x.2018.08.022

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 8: 3055 - 3062

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.08.022

Molecular Nutrition

Effects of Weaning Days of Age on Ruminal Microbial Diversity in Hu Lambs

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1. Key Laboratory of Grassland Farming Systems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
2. New Hope Liuhe Group Co., Ltd., Beijing 100102, China;
3. Biotechnology Engineering Laboratory of Gansu Meat Sheep Breeding, Minqin 733300, China

Received date: 2018-01-26

Online published: 2018-08-18

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Abstract

The aim of the present study was to evaluate the weaning days of age on ruminal microbial diversity in Hu lambs. Sixty-six male Hu lambs were selected, and six lambs were slaughtered at each sample time (1, 14 and 28 days of age, respectively). The rest 48 lambs were divided into two groups weaned at 28 and 56 days of age, respectively. Lambs were slaughtered at 42, 56, 70 and 84 days of age (6 lambs per group at each sample time), respectively, to collect ruminal content. All lambs were fed starter since 7 d of age. The experiment period was from 1 to 84 days of age. The results showed as follow:weaning days of age had no significant effects on the relative abundance of Ruminococcus albus, Ruminococcus flavefaciens, Streptococcus bovis, Fibrobacter succinogenes and Treponema bryantii. With the increase of days of age, ruminal microbial diversity of lambs continuously increased; compared with lambs weaned at 56 days of age, lambs weaned at 28 days of age had higher microbial diversity. The results indicate that the microbial diversity are affected by days of age and weaning days of age, and early weaning can accelerate establishment of ruminal microflora.

Key words： Hu lambs; weaning days of age; microbial diversity

Cite this article

MA Wanhao, ZHANG Ning, LI Fei, LI Fadi . Effects of Weaning Days of Age on Ruminal Microbial Diversity in Hu Lambs[J]. Chinese Journal of Animal Nutrition, 2018 , 30(8) : 3055 -3062 . DOI: 10.3969/j.issn.1006-267x.2018.08.022

References

[1] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2006.

[2] 淡瑞芳,张海涛,龙瑞军,等.瘤胃微生物生态研究方法评述[J].草业科学,2007,24(7):77-82.

[3] DE FILIPPO C,CAVALIERI D,DI PAOLA M,et al.Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa[J].Proceedings of The National Academy of Sciences of the United States of America,2010,107(33):14691-14696.

[4] 陈昊,刘婷,吴建平,等.牛至精油对新生犊牛生长发育和血液免疫的影响[J].草业科学,2017,34(10):2141-2148.

[5] 罗惦,柴林荣,常生华,等.我国青藏高原地区牦牛草地放牧系统管理及优化[J].草业科学,2017,34(4):881-891.

[6] 宫曼丽,任南琪,邢德峰.DGGE/TGGE技术及其在微生物分子生态学中的应用[J].微生物学报,2004,44(6):845-848.

[7] FIRKINS J L,YU Z.Ruminant nutrition symposium:how to use data on the rumen microbiome to improve our understanding of ruminant nutrition[J].Journal of Animal Science,2015,93(6):1450-1470.

[8] KHAFIPOUR E,LI S C,PLAIZIER J C,et al.Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis[J].Applied and Environmental Microbiology,2009,75(22):7115-7124.

[9] LEY R E,HAMADY M,LOZUPONE C,et al.Evolution of mammals and their gut microbes[J].Science,2008,320(5883):1647-1649.

[10] 张宁.断奶和补饲时间对湖羊羔羊肌肉脂肪酸和瘤胃微生物多样性的影响[D].硕士学位论文.兰州:兰州大学,2016.

[11] 周奕毅.茶皂素抑制湖羊甲烷生成的微生物学机制研究[D].硕士学位论文.杭州:浙江大学,2009.

[12] JAMI E,ISRAEL A,KOTSER A,et al.Exploring the bovine rumen bacterial community from birth to adulthood[J].The ISME Journal,2013,7(6):1069-1079.

[13] 马志远.早期断奶对湖羊羔羊生产性能及胃肠道发育的影响[D].硕士学位论文.兰州:兰州大学,2015.

[14] 柴建民.断母乳日龄对羔羊生长性能与胃肠道发育的影响[D].硕士学位论文.北京:中国农业科学院,2015.

[15] 韩旭峰.日龄、日粮精粗比对陕北白绒山羊瘤胃微生物区系影响的研究[D].博士学位论文.杨凌:西北农林科技大学,2015.

[16] MARTIN C,MICHALET-DOREAU B.Variations in mass and enzyme activity of rumen microorganisms:effect of barley and buffer supplements[J].Journal of The Science of Food and Agriculture,1995,67(3):407-413.

[17] CHEN Y H.Changes in ruminal bacterial communities of beef cattle during high concentrated diet transition and experimental induced subacute acidosis[D].Master's Thesis.Edmonton:University of Alberta,2011.

[18] TAJIMA K,AMINOV R I,NAGAMINE T,et al.Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR[J].Applied and Environmental Microbiology,2001,67(6):2766-2774.

[19] 张红涛.不同玉米青贮水平对荷斯坦后备牛瘤胃液微生物组及其代谢组的影响[D].博士学位论文.北京:中国农业大学,2017.

[20] KHAN M A,BACH A,WEARY D M,et al.Invited review:transitioning from milk to solid feed in dairy heifers[J].Journal of Dairy Science,2016,99(2):885-902.

[21] EL-SHAZLY K,HUNGATE R E.Fermentation capacity as a measure of net growth of rumen microorganisms[J].Applied Microbiology,1965,13:62-93.

[22] LIU T,LI F D,WANG W M,et al.Effects of lamb early starter feeding on the expression of genes involved in volatile fatty acid transport and pH regulation in rumen tissue[J].Animal Feed Science and Technology,2016,217:27-35.

[23] BOMBA A,KMET V,KONIAROVÁ I,et al.Production of volatile fatty acids in the rumen of calves during dietary-microbial stimulation and early weaning[J].Veterinární Medicina,1989,34(3):141-148.

[24] YÁÑEZ-RUIZ D R,ABECIA L,NEWBOLD C J.Manipulating rumen microbiome and fermentation through interventions during early life:a review[J].Frontiers in Microbiology,2015,34:1133.

[25] REY M,ENJALBERT F,COMBES S,et al.Establishment of ruminal bacterial community in dairy calves from birth to weaning is sequential[J].Journal of Applied Microbiology,2014,116(2):245-257.

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