Effects of Small Peptides on Growth, Body Composition, Non-Specific Immunity and Disease Resistance of Juvenile Litopenaeus vannamei

LI Rimei, SHEN Guangrong, HUANG Fang, YANG Qihui, TAN Beiping, DONG Xiaohui

doi:10.3969/j.issn.1006-267x.2018.08.025

2018 , Vol. 30 >Issue 8: 3082 - 3090

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.08.025

Molecular Nutrition

Effects of Small Peptides on Growth, Body Composition, Non-Specific Immunity and Disease Resistance of Juvenile Litopenaeus vannamei

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1. Laboratory of Aquatic Animal Nutrition and Feed, Guangdong Ocean University, Zhanjiang 524088, China;
2. Shenzhen Yunong Science & Technology Co., Ltd., Shenzhen 518110, China

Received date: 2018-01-15

Online published: 2018-08-18

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Abstract

A 56-day feeding trial was carried out to investigate the effects of small peptides on growth, body composition, non-specific immunity and disease resistance of juvenile Litopenaeus vannamei. Six experimental diets were prepared by adding 0 (control), 0.5%, 1.0%, 2.0%, 4.0% and 6.0% of small peptides into the basal diet, and named as S0, S0.5, S1.0, S2.0, S4.0 and S6.0, respectively. A total of 540 Litopenaeus vannamei with the initial body weight of (0.19±0.01) g were randomly assigned into 6 groups with 3 replicates per group and 30 individuals per replicate. The results showed that the weight gain rate (WGR), specific growth rate (SGR) and protein efficiency rate (PER) in small peptides adding groups were significantly higher than those in control group (P<0.05), while the feed conversion rate (FCR) in small peptides adding groups was significantly lower than those in control group (P<0.05); however, the survival rate (SR) of shrimp had no significant difference among groups (P>0.05). In serum, phenoloxidase (PO) activity in S1.0 and S2.0 groups was significantly higher than that in other groups (P<0.05); superoxide dismutase (SOD) activity in S0.5 and S1.0 groups was significantly higher than that in control group (P<0.05); acid phosphatase (ACP) activity in S1.0 and S2.0 groups was significantly higher than that in control group (P<0.05); the activities of alkaline phosphatase (AKP) and lysozyme (LSZ) were not significantly different among groups (P>0.05); malondialdehyde (MDA) content in S4.0 and S6.0 groups was significantly lower than that in other groups (P<0.05). In hepatopancreas, LSZ activity in S0.5, S2.0 and S4.0 groups was significantly higher than that in control group (P<0.05), but it in S6.0 group was significantly lower than that in control group (P<0.05); SOD activity was not significantly different among groups (P>0.05); ACP activity in S1.0 and S0.5 groups was higher, but compared with control group had no significant difference (P>0.05); AKP activity in S4.0 group was significantly lower than that in control group (P<0.05); MDA content in small peptides adding groups was significantly lower than that in control group (P<0.05), and it in S4.0 group was significantly lower than that in S6.0 group (P<0.05). Vibrio harveyi challenge test showed that the cumulative mortality rate after 7 days challenged by Vibrio harveyi in groups with the small peptides addition of 0.5% to 4.0% was significantly lower than that in control group (P<0.05), but it in group with the small peptides addition of 6.0% had no significant difference compared with control group (P>0.05). In summary, adding 1.0% to 2.0% small peptides into the diet can significantly improve the growth, and increase the non-specific immunity and disease resistance of juvenile Litopenaeus vannamei.

Key words： small peptides; Litopenaeus vannamei; growth; non-specific immunity; disease resistance

Cite this article

LI Rimei, SHEN Guangrong, HUANG Fang, YANG Qihui, TAN Beiping, DONG Xiaohui . Effects of Small Peptides on Growth, Body Composition, Non-Specific Immunity and Disease Resistance of Juvenile Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 2018 , 30(8) : 3082 -3090 . DOI: 10.3969/j.issn.1006-267x.2018.08.025

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