Molecular Nutrition

Effects of Cecal Infusion of Propionate on Gene Expression in Colon Mucosa of Growing Pigs

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  • Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2018-01-31

  Online published: 2018-08-18

Abstract

The aim of this experiment was to explore the effects of cecal infusion of propionate on gene expression in colon mucosa of growing pigs via RNA sequencing (RNA-seq). Sixteen growing barrows (Duroc×Landrace×Large White) were divided into control group (n=8) and experimental group (n=8) randomly. The pigs from the experimental group were infused with propionate which was formulated with saline through the cecal fistula, while the pigs from the control group were infused with the same amount of saline for 28 days. After the infusion, pigs were slaughtered, and their colon mucosas were collected for RNA-seq analysis. The results showed that there were 121 differentially expressed genes in colon mucosa after infusion of propionate (FC ≥ 2, P<0.05), with 78 up-regulated and 43 down-regulated genes. In which, propionate significantly increased the expression of glucose-and energy-related genes, including peptide YY (PYY), insulin-like growth factor binding protein-7 (IGFBP7), fructose-bisphosphate aldolase (ALDOB), ATP synthase 5I (ATP5I), nicotinamide adenine dinucleotide dehydrogenase subunit 4L (ND4L) and cytochrome P450 (CYP39A1) (P<0.05). In addition, the expression of immune-related genes, such as Occludin-1, somatostatin (SST) and G protein-coupled receptor (GPRC5A) was also increased (P<0.05). GO and KEGG pathway analysis showed that the up-regulated DEGs significantly enriched GO term involved in glycosyl compound metabolism, carbohydrate derivative metabolism, etc, and significantly enriched pathways involved in oxidative phosphorylation, glycosaminoglycan biosynthesis, etc; the down-regulated DEGs significantly enriched GO term involved in cardiac chamber development, stem cell population maintenance etc, and significantly enriched pathways involved in taurine and hypotaurine metabolism, folate biosynthesis, etc. Taken together, cecal infusion of propionate alters the expression of glucose-and energy-related genes, and intestinal barrier-and immune-related genes, suggesting a crucial role of propionate in colonic metabolism and host health.

Cite this article

CHEN Huizi, ZHANG Yanan, YU Kaifan, ZHU Weiyun . Effects of Cecal Infusion of Propionate on Gene Expression in Colon Mucosa of Growing Pigs[J]. Chinese Journal of Animal Nutrition, 2018 , 30(8) : 3134 -3141 . DOI: 10.3969/j.issn.1006-267x.2018.08.031

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