Effects of Vitamin A on Gene Expressions Related to Milk Fat and Protein Synthesis in Bovine Mammary Epithelial Cells

SU Rui, LIU Yang, YAN Sumei, SHI Binlin, ZHAO Yanli, SHI Huiyu

doi:10.3969/j.issn.1006-267x.2018.08.033

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 8: 3151 - 3158

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.08.033

Molecular Nutrition

Effects of Vitamin A on Gene Expressions Related to Milk Fat and Protein Synthesis in Bovine Mammary Epithelial Cells

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College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2018-02-01

Online published: 2018-08-18

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Abstract

This experiment was conducted to investigate the effects of vitamin A on gene expresions related to milk fat and protein synthesis in bovine mammary epithelial cells (BMECs). The experiment was designed as a single factor randomized trial, and the 3rd generation BMECs were randomly divided into 6 treatments with 6 replicates per group. After cultured in culture medium without serum for 24 h, cells in different treatments were cultured in culture medium supplemented with 0(control), 0.05, 0.10, 0.20, 1.00 and 2.00 μg/mL vitamin A for 24 h, respectively. The results showed that compared with the control group, 1.00 and 2.00 μg/mL vitamin A could significantly increase relative growth rate and triacylglycerols (TG) content (P<0.05); vitamin A could significantly up-regulate expression levels of genes related to milk fat synthesis, such as peroxisome proliferator-activated receptor γ (PPARG, 0.05, 0.10, 0.20, 1.00 and 2.00 μg/mL), sterol regulatory element-binding protein 1 (SREBP1, 0.05 and 0.10 μg/mL), stearoyl-CoA desaturase (SCD, 0.05 and 0.10 μg/mL) (P<0.05); vitamin A could significantly up-regulate expression levels of genes related to milk protein synthesis, such as signal transducers and activators of transcription 5 (STAT5, 0.20 μg/mL), α-casein (CSN1S1, 0.05 and 0.10 μg/mL) and κ-casein (CSN3, 0.10 μg/mL) (P<0.05); vitamin A could significantly increase acetylcoenzyme A (ACACA) activity (0.05, 0.10, 0.20 and 1.00 μg/mL) (P<0.05), which was related to milk fat synthesis. In conclusion, vitamin A has concentration-dependent effects on expressions of genes related to milk fat and protein synthesis in BMECs, and the promotion effects of 0.10 μg/mL vitamin A are better.

Key words： vitamin A; milk fat; milk protein; gene expression

Cite this article

SU Rui, LIU Yang, YAN Sumei, SHI Binlin, ZHAO Yanli, SHI Huiyu . Effects of Vitamin A on Gene Expressions Related to Milk Fat and Protein Synthesis in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2018 , 30(8) : 3151 -3158 . DOI: 10.3969/j.issn.1006-267x.2018.08.033

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