Effects of Oregano Oil on Ruminal Fermentation Characteristics and Methane Production of Sheep by Gas Production Technique in Vitro

ZHANG Ran, ZHENG Chen, YAN Xiaogang, LIANG Hao, YANG Huaming

doi:10.3969/j.issn.1006-267x.2018.08.035

2018 , Vol. 30 >Issue 8: 3168 - 3175

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.08.035

Molecular Nutrition

Effects of Oregano Oil on Ruminal Fermentation Characteristics and Methane Production of Sheep by Gas Production Technique in Vitro

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1. Branch Academy of Animal Science, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China;
2. College of Animal Science and Technology, Gansu Agriculture University, Lanzhou 730070, China

Received date: 2018-01-29

Online published: 2018-08-18

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Abstract

This study aimed to explore the effects of oregano oil on ruminal fermentation characteristics and methane production of sheep by gas production technique in vitro. A single-factor experimental design was used. Oregano oil was added in culture medium for different groups at levels of 0 (control), 100, 200, 300 and 400 mg/L, respectively. Each group had 5 replicates. After fermented for 12 h, methane production, total gas production, as well as pH, concentrations of total nitrogen, ammonia nitrogen, urea nitrogen and protein nitrogen, and concentrations and proportions of volatile fatty acids in culture medium were measured. The results showed as follows:1) the addition of oregano oil at levels of 100, 200, 300 and 400 mg/L all significantly increased culture medium pH (P<0.05); the addition of high levels (300 and 400 mg/L) of oregano oil significantly decreased the concentration of total volatile fatty acids and the proportion of propionate (P<0.05), and significantly increased the proportion of acetate and the ratio of acetate to propionate in culture medium (P<0.05). 2) Compared with control group, the concentration of ammonia nitrogen was significantly decreased in experimental groups except 100 mg/L oregano oil group (P<0.05). 3) The addition of high levels (300 and 400 mg/L) of oregano oil significantly decreased methane production and total gas production (P<0.05). In conclusion, oregano oil can regulate ruminal fermentation and reduce methane production, but the over-high addition level had inhibination effects on ruminal fermentation, and the optimum adding level is 200 mg/L under in vitro conditions.

Key words： oregano oil; gas production technique in vitro; fermentation characteristic; methane yield

Cite this article

ZHANG Ran, ZHENG Chen, YAN Xiaogang, LIANG Hao, YANG Huaming . Effects of Oregano Oil on Ruminal Fermentation Characteristics and Methane Production of Sheep by Gas Production Technique in Vitro[J]. Chinese Journal of Animal Nutrition, 2018 , 30(8) : 3168 -3175 . DOI: 10.3969/j.issn.1006-267x.2018.08.035

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