Molecular Nutrition

Effects of Curcumin and Bacillus licheniformis on Growth Performance, Serum Antioxidant Function, Intestinal Microbe Counts and Immune Organ Indexes of Broilers

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  • Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

Received date: 2018-01-04

  Online published: 2018-08-18

Abstract

This experiment was conducted to determine the effects of curcumin and Bacillus licheniformis on growth performance, serum antioxidant function, intestinal microbe counts and immune organ indexes of broilers. A total of 450 Arbor Acres (AA) broilers at one day age were randomly divided into 5 groups with 6 replicates per group and 15 broilers per replicate. The broilers of control group were fed a basal diet, and those in experimental groups were fed the basal diet supplemented with 35 mg/kg antibiotic (D1 group), 200 mg/kg curcumin (D2 group), 100 mg/kg Bacillus licheniformis (D3 group) and 100 mg/kg Bacillus licheniformis+200 mg/kg curcumin (D4 group), respectively. The experiment lasted for 42 days. The results showed as follows:1) compared with control group, average daily gain (ADG) of experimental groups was significantly increased (P<0.05); ADG and final weight of D1 group were significantly higher than those of D2 and D3 groups (P<0.05), but had no significant differences compared with D4 group (P<0.05); feed to gain ratio of D1 and D4 groups was significantly lower than that of control group (P<0.05). 2) Compared with D1 group and control group, serum activities of lysozyme (LZM), superoxide dismutase (SOD) (except D3 group) and glutathione peroxidase (GSH-Px) were significantly increased in D2, D3 and D4 groups (P<0.05); serum malondialdehyde (MDA) content of D2, D3 and D4 groups was significantly lower than that of control group (P<0.05). 3) Compared with control group, the counts of Lactobacillus and Bacillus bifidus in intestinal of D4 group were significantly increased (P<0.05), but there were no significant differences among experimental groups (P>0.05); the counts of Escherichia coli and Salmonella in intestinal of D1 and D4 groups were significantly decreased (P<0.05). 4) Compared with control group, spleen and bursa of Fabricius indexes of D4 group were significantly increased (P<0.05). The results indicate that the addition of curcumin and Bacillus licheniformis alone or combined in diets can improve growth performance, immune function and intestinal microbial environment of broilers, and the combination effect is better than alone effect, which suggests a synergistic reaction between them.

Cite this article

SUN Quanyou, LI Wenjia, XU Bin, WEI Fengxian, WANG Linyi, LIN Ping, LI Shaoyu . Effects of Curcumin and Bacillus licheniformis on Growth Performance, Serum Antioxidant Function, Intestinal Microbe Counts and Immune Organ Indexes of Broilers[J]. Chinese Journal of Animal Nutrition, 2018 , 30(8) : 3176 -3183 . DOI: 10.3969/j.issn.1006-267x.2018.08.036

References

[1] DIBAISE J K,ZHANG H S,CROWELL M D,et al.Gut microbiota and its possible relationship with obesity[J].Mayo Clinic Proceedings,2008,83(4):460-469.  

[2] VERSTEGEN M W,WILLIAMS B A.Alternatives to the use of antibiotics as growth promoters for monogastric animals[J].Animal Biotechnology,2002,13(1):113-127.  

[3] STANKOVIC I.61st Joint expert committee on food additives[J].Chemical and Technical Assessment,2004,1(8):1-8.

[4] SA G,DAS T.Anti cancer effects of curcumin:cycle of life and death[J].Cell Division,2008,3(1):14.

[5] PRIYADARSINI K I,MAITY D K,NAIK G H,et al.Role of phenolic O-H and methylene hydrogen on the free radical reactions and antioxidant activity of curcumin[J].Free Radical Biology and Medicine,2003,35(5):475-484.  

[6] MIRIYALA S,PANCHATCHARAM M,RENGARAJULU P.Cardioprotective effects of curcumin[C]//AGGARWAL B B,SURH Y J,SHISHODIA S.The molecular targets and therapeutic uses of curcumin in health and disease.Boston:Springer,2007:359-377.

[7] HATCHER H,PLANALP R,CHO J,et al.Curcumin:from ancient medicine to current clinical trials[J].Cellular and Molecular Life Sciences,2008,65(11):1631-1652.  

[8] 张菊,李金敏,张志焱,等.地衣芽孢杆菌的研究进展[J].中国饲料,2012(17):9-11.

[9] BENYACOUB J,CZARNECKI-MAULDEN G L,CAVADINI C,et al.Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs[J].The Journal of Nutrition,2003,133(4):1158-1162.  

[10] 祝国强,侯风琴.姜黄素对肉仔鸡日增重、脂质代谢、肉品质的影响[J].饲料博览,2006(2):49-51.

[11] 胡忠泽,金光明,王立克,等.姜黄素对肉鸡生产性能和免疫机能的影响[J].粮食与饲料工业,2004(10):44-45.

[12] 彭翔.抗菌肽和姜黄素对肉仔鸡生长性能、抗氧化功能、肠道微生物及免疫功能的影响[J].硕士学位论文.郑州:河南农业大学,2014.

[13] 杨泰,王慧,田科雄,等.姜黄素的生理功能及其在畜禽生产中的应用[J].动物营养学报,2017,29(10):3460-3466.

[14] ZHANG J F,HU Z P,LU C H,et al.Dietary curcumin supplementation protects against heat-stress-impaired growth performance of broilers possibly through a mitochondrial pathway[J].Journal of Animal Science,2015,93(4):1656-1665.  

[15] GOWDA N K S,LEDOUX D R,ROTTINGHAUS G E,et al.Antioxidant efficacy of curcuminoids from turmeric (Curcuma longa L.) powder in broiler chickens fed diets containing aflatoxin B1[J].British Journal of Nutrition,2009,102(11):1629-1634.  

[16] MANICKAM R,VISWANATHAN K,MOHAN M.Effect of probiotics in broiler performance[J].Indian Veterinary Journal,1994,71(7):737-739.

[17] 杨家军,钱坤,章薇,等.地衣芽孢杆菌对肉鸡生产性能,抗氧化及营养物质代谢的影响研究[J].中国兽医学报,2014,34(5):736-739.

[18] MANABE K,KAGEYAMA Y,MORIMOTO T,et al.Improved production of secreted heterologous enzyme in Bacillus subtilis strain MGB874 via modification of glutamate metabolism and growth conditions[J].Microbial Cell Factories,2013,12(1):18.

[19] 利明.日粮中添加芽孢杆菌对肉仔鸡生产性能和免疫功能影响的研究[D].硕士学位论文.呼和浩特:内蒙古大学,2009:37-39.

[20] WEI X B,LIU H Q,SUN X,et al.Hydroxysafflor yellow A protects rat brains against ischemia-reperfusion injury by antioxidant action[J].Neuroscience Letters,2005,386(1):58-62.  

[21] 胡春生,陈炜林,易传祝,等.姜黄素抗氧化作用人体试食研究[J].湖南中医药大学学报,2007,27(5):63-64.

[22] 李婉雁,陈国开,庞木生,等.姜黄粉对岭南黄鸡生产性能、免疫器官指数和抗氧化能力的影响[J].仲恺农业工程学院学报,2010,23(3):36-39.

[23] SOOD P K,NAHAR U,NEHRU B.Curcumin attenuates aluminum-induced oxidative stress and mitochondrial dysfunction in rat brain[J].Neurotoxicity Research,2011,20(4):351-361.  

[24] DUC L H,HONG H A,BARBOSA T M,et al.Characterization of Bacillus probiotics available for human use[J].Applied and Environmental Microbiology,2004,70(4):2161-2171.  

[25] 王放银.用草药和植物提取物作生长促进剂[J].湖南饲料,2000(3):24-26.

[26] 郑召岭.姜黄素抗炎及免疫调节作用研究进展[J].山西中医,2008,24(3):48-49.

[27] 孙全友,魏凤仙,李绍钰.姜黄素在肉鸡生产中的应用[J].中国家禽,2013,35(6):54-56.

[28] 钟英英,黄晓畅,陈世益.姜黄素的体外抑菌活性研究[J].安徽农业科学,2010,38(34):19369-19370,19377.

[29] 赵娜,申杰,魏金涛,等.凝结芽孢杆菌对肉鸡生长性能、免疫器官指数、血清生化指标及肠道菌群的影响[J].动物营养学报,2017,29(1):249-256.

[30] 齐博,武书庚,王晶,等.枯草芽孢杆菌对肉仔鸡生长性能、肠道形态和菌群数量的影响[J].动物营养学报,2016,28(6):1748-1756.

[31] TEO A Y,TAN H M.Evaluation of the performance and intestinal gut microflora of broilers fed on corn-soy diets supplemented with Bacillus subtilis PB6(CloSTAT)[J].The Journal of Applied Poultry Research,2007,16(3):296-303.  

[32] 何明清.动物微生态学[M].北京:中国农业出版社,1994:69.

[33] FLUHARTY F L,MCCLURE K E,SOLOMON M B,et al.Energy source and ionophore supplementation effects on lamb growth,carcass characteristics,visceral organ mass,diet digestibility,and nitrogen metabolism[J].Journal of Animal Science,1999,77(4):816-823.  

[34] 张婧菲.姜黄素对动物线粒体氧化损伤的保护作用及其抗氧化机制研究[D].博士学位论文.南京:南京农业大学,2015:72-73.

[35] 狄建彬,顾振纶,赵笑东,等.姜黄素的抗氧化和抗炎作用研究进展[J].中草药,2010,41(5):854-857.

[36] 石宁.地衣芽孢杆菌和低聚木糖及乳酸对肉鸡肠绒组织及肠道菌群的影响[D].硕士学位论文.郑州:河南工业大学,2010:46-48.

[37] 孙小沛,杨在宾,李兆勇,等.地衣芽孢杆菌与日粮蛋白水平对肉鸡生产性能、肠道环境及免疫器官指数的影响[J].饲料工业,2013,34(23):40-46.

[38] 李福彬.地衣芽孢杆菌的理化特性及对蛋鸡生产性能影响机理的研究[D].硕士学位论文.保定:河北农业大学,2010:30-33.
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