Effects of High Water-Soluble Mono-Calcium and Di-Calcium Phosphate on Growth Performance, Dietary Digestibility, Serum and Bone Indices of Weaned Piglets

LYU Xiaokang, CHAI Jianmin, WANG Jie, SI Bingwen, CUI Kai, WANG Shiqin, TU Yan, DIAO Qiyu, ZHANG Naifeng

doi:10.3969/j.issn.1006-267x.2018.09.002

2018 , Vol. 30 >Issue 9: 3337 - 3352

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.09.002

Molecular Nutrition

Effects of High Water-Soluble Mono-Calcium and Di-Calcium Phosphate on Growth Performance, Dietary Digestibility, Serum and Bone Indices of Weaned Piglets

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Feed Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, China

Received date: 2017-12-01

Online published: 2018-09-20

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Abstract

This study was conducted to determine the relative biological value (RBV) of mono-calcium and di-calcium phosphate (MDCP) by using dibasic calcium phosphate (DCP) as the reference and taking the growth performance, dietary digestibility, serum and bone indices of weaned piglets as indicators. With a 2×5 factorial arrangement experimental design, four hundred and thirty-two Duroc×Large White×Yorkshire weaned piglets with similar body weight and 4 to 5 weeks of age were randomly allocated into 9 groups with 6 replicates in each group and 8 piglets in each replicate. The phosphates were added into a basal diet at 5 phosphorus supplemental levels as 0, 0.05%, 0.10%, 0.15% and 0.20% (0 supplemental group was shared), respectively, and there were 9 treatments in all. The adaptation period lasted for 3 days and the experimental period lasted for 35 days. The results showed as follows:1) no significant interaction was found between phosphorus source and phosphorus supplemental level on the average daily feed intake, average daily gain and mortality of piglets (P>0.05). The average daily feed intake of piglets in MDCP group was significantly higher than that in DCP group during 15 to 35 days of experiment (P<0.05). With the increasing of dietary phosphorus levels, the average daily gain and average daily feed intake were significantly increased (P<0.05), but the feed conversion rate was not significantly changed (P>0.05). 2) There was no significant interaction between phosphates source and phosphorus supplemental level on the apparent digestibility of dry matter, calcium and phosphorus in piglets (P>0.05). The apparent digestibility of calcium and phosphorus in MDCP group was significantly higher than that in DCP group (P<0.05). The apparent digestibility of calcium in 0.10%, 0.15% and 0.20% phosphorus supplemental groups was significantly higher than that in 0 supplemental group (P<0.05). The RBVs of MDCP calculated by the slope ratio were both 120% regarding the apparent digestibility and true digestibility of phosphorus as indicators and DCP as the reference. 3) At the 14^th and 35^th day of experiment, the serum alkaline phosphatase (ALP) activity of piglets were not significantly affected by different phosphorus sources and supplemental levels (P>0.05), and there was no significant interaction either (P>0.05). At the 14^th day of experiment, serum contents phosphorus, calcium and parathyroid hormone (PTH) were significantly interacted between different phosphorus source and phosphorus supplemental level (P<0.05), but there was no significant interaction at the 35^th day of experiment (P<0.05). At the 14^th day of experiment, the serum calcium content in MDCP group was significantly higher than that in DCP group (P<0.05), and the serum calcitonin (CT) content in MDCP group was significantly lower than that in DCP group (P<0.05). At the 14^th and 35^th day of experiment, the serum CT content showed no significant interaction between different phosphates and different phosphorus supplemental level (P>0.05). There was a significant linear regression relationship between the serum contents of calcium, phosphorus, PTH and phosphorus supplemental level (P<0.05). With regression equations established by using serum contents of calcium, phosphorus and PTH as indicators, the RBVs of MDCP calculated by the slope ratio of regression equation were 96%, 102% and 115% (mean was 104%), respectively. 4) There was no significant difference in the bone fracture strength of femur, tibia and metacarpal of different phosphates and different phosphorus supplemental level (P>0.05) and no significant interaction either (P>0.05). There was a significant linear regression between femur ash content and phosphorus supplemental level (P<0.05). Based on the femur ash content, the RBV of MDCP calculated by the slope ratio of regression equation was 255%. In summary, taking the RBV of reagent grade DCP as 100%, based on apparent digestibility and true digestibility of phosphorus, the RBVs of MDCP are both 120%; and based on the serum indices and femur ash content, the RBVs of MDCP are 104% and 255%, respectively.

Key words： weaned piglet; mono-calcium and di-calcium phosphate; dibasic calcium phosphate; growth performance; digestibility; serum indices; bone indices; relative biological value

Cite this article

LYU Xiaokang, CHAI Jianmin, WANG Jie, SI Bingwen, CUI Kai, WANG Shiqin, TU Yan, DIAO Qiyu, ZHANG Naifeng . Effects of High Water-Soluble Mono-Calcium and Di-Calcium Phosphate on Growth Performance, Dietary Digestibility, Serum and Bone Indices of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3337 -3352 . DOI: 10.3969/j.issn.1006-267x.2018.09.002

References

[1] 万荣,何姝颖,谢木林.不同无机磷源对仔猪腹泻及肠道微生态的影响[J].饲料研究,2015(23):25-28.

[2] EECKHOUT W,DE PAEPE M.The digestibility of three calcium phosphates for pigs as measured by difference and by slope-ratio assay[J].Journal of Animal Physiology and Animal Nutrition,1997,77(1/2/3/4/5):53-60.

[3] 谭占坤.磷来源和水平对蛋鸡生产性能、蛋品质和骨骼质量的影响[D].硕士学位论文.雅安:四川农业大学,2011.

[4] PETERSEN G I,PEDERSEN C,LINDEMANN M D,et al.Relative bioavailability of phosphorus in inorganic phosphorus sources fed to growing pigs[J].Journal of Animal Science,2011,89(2):460-466.

[5] 曹慧.猪对饲料级磷酸盐磷生物学利用率的研究[D].硕士学位论文.雅安:四川农业大学,2003.

[6] 夏良宙,李霞,万荣.不同种类和来源磷酸钙盐对肉鸡生产性能和养分代谢的影响[J].饲料工业,2014(增刊):48-52.

[7] 万荣,谢木林,何姝颖.不同无机磷源对仔猪生产性能和表观消化率的影响[J].饲料研究,2015(21):19-22,51.

[8] 万荣,谢木林.新型磷源Ⅲ型磷酸氢钙在肉鸡上相对生物学利用率的应用研究[J].饲料广角,2014(16):16-19.

[9] 陈晓春,姜光丽,周光荣.磷酸一二钙在产蛋鸡配合饲料中的应用研究[J].中国家禽,2007,29(22):12-15.

[10] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社,2003.

[11] 邓雪娟,刘国华,蔡辉益,等.分光光度计法测定家禽饲料和食糜中二氧化钛[J].饲料工业,2008,29(2):57-58.

[12] 罗赞,贺建华,田科雄,等.线性回归法测定生长猪内源磷排泄量及鱼粉磷真消化率[J].动物营养学报,2009,21(4):441-446.

[13] FAN M Z,ARCHBOLD T,SAUER W C,et al.Novel methodology allows simultaneous measurement of true phosphorus digestibility and the gastrointestinal endogenous phosphorus outputs in studies with pigs[J].The Journal of Nutrition,2001,131(9):2388-2396.

[14] SHEN Y R,FAN M Z,AJAKAIYE A,et al.Use of the regression analysis technique to determine the true phosphorus digestibility and the endogenous phosphorus output associated with corn in growing pigs[J].The Journal of Nutrition,2002,132(6):1199-1206.

[15] LITTELL R C,HENRY P R,LEWIS A J,et al.Estimation of relative bioavailability of nutrients using SAS procedures[J].Journal of Animal Science,1997,75(10):2672-2683.

[16] CROMWELL G L.Biological availability of phosphorus for pigs[J].Feedstuffs,1980,52(9):38-42.

[17] DE OLIVEIRA SOUZA L W,DE SANT'ANNA MORETTI A,TUCCI F M,et al.Phosphorus availability of rock phosphates as compared with feed-grade phosphates for swine[J].Revista Brasileira de Zootecnia,2009,38(1):90-98.

[18] 黄阿彬.不同磷源及水平对育肥猪生长性能和骨骼质量的影响[D].硕士学位论文.雅安:四川农业大学,2013.

[19] 何姝颖,万荣.猪对钙磷营养代谢研究进展[J].饲料广角,2016(1):28-31.

[20] CROMWELL G L.The biological availability of phosphorous in feedstuffs for pigs[J].Pig News and Information,1992,13:75-78.

[21] BAKER S R,KIM B G,STEIN H H.Comparison of values for standardized total tract digestibility and relative bioavailability of phosphorus in dicalcium phosphate and distillers dried grains with solubles fed to growing pigs[J].Journal of Animal Science,2013,91(1):203-210.

[22] NEWMAN C W,ELLIOTT D O.Source and level of phosphorus for growing-finishing swine[J].Journal of Animal Science,1976,42(1):92-98.

[23] 王秀静.不同无机磷源对断奶仔猪生长性能、肠道形态结构及肠道稳态的影响[D].硕士学位论文.南昌:江西农业大学,2015.

[24] 贾海康.不同磷酸盐对肉鸡的适宜用量研究[D].硕士学位论文.雅安:四川农业大学,2016.

[25] JONGBLOED A W.Phosphorus in the feeding of pigs:effect of diet on the absorption and retention of phosphorus by growing pigs[D].Ph.D.Thesis.Lelystad:Agricultural University,1987.

[26] GRIMBERGEN A H M,CORNELISSEN J P,STAPPERS H P.The relative availability of phosphorus in inorganic feed phosphates for young turkeys and pigs[J].Animal Feed Science and Technology,1985,13(1/2):117-130.

[27] DILGER R N,ADEOLA O.Estimation of true phosphorus digestibility and endogenous phosphorus loss in growing pigs fed conventional and low-phytate soybean meals[J].Journal of Animal Science,2006,84(3):627-634.

[28] PETTEY L A,CROMWELL G L,LINDEMANN M D.Estimation of endogenous phosphorus loss in growing and finishing pigs fed semi-purified diets[J].Journal of Animal Science,2006,84(3):618-626.

[29] 王顺祥,印遇龙,李铁军,等.回归法测定生长猪内源磷排泄量及磷真消化率研究[J].西北农林科技大学学报(自然科学版),2007,35(7):33-38.

[30] 张艳玲.利用线性回归法测定小母猪和阉公猪内源钙、磷排泄量和豆粕钙、磷真消化率的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2004.

[31] AMMERMAN C B,BAKER D H,LEWIS A J.Bioavailability of nutrients for animals:amino acids,minerals,and vitamins[M].San Diego:Academic Press,1995.

[32] DELLAERT B M,VAN DER PEET G F,JONGBLOED A W,et al.A comparison of different techniques to assess the biological availability of feed phosphates in pig feeding[J].Netherlands Journal of Agricultural Science,1990,38(3):555-566.

[33] RODEHUTSCORD M,FAUST M,DVNGELHOEF M,et al.Zur messung der verdaulichkeit des phosphors aus mineralischen phosphor-trägern sowie aus mineralfuttern,eiweiβ konzentraten und alleinfuttern für schweine[J].Journal of Animal Physiology and Animal Nutrition,2010,71(1/2/3/4/5):169-178.

[34] PETERSEN G I,STEIN H H.Novel procedure for estimating endogenous losses and measurement of apparent and true digestibility of phosphorus by growing pigs[J].Journal of Animal Science,2006,84(8):2126-2132.

[35] JONGBLOED A W,EVERTS H,KEMME P A.Phosphorus availability and requirements in pigs[M]//HARESIGN W,COLE D J A,eds.Recent Advances in Animal Nutrition.Amsterdam:Elsevier,1991:65-80.

[36] 刘静波,曹山川,黄溢,等.生长猪基础饲粮类型对饲粮无机磷的磷真消化率的影响[J].中国畜牧杂志,2016,52(13):43-47.

[37] 霍启光.动物磷营养与磷源[M].北京:中国农业科学技术出版社,2002.

[38] ZWART S.Bio-availability of feed phosphates[J].Feed Compounder,1999,19(2):34-38.

[39] WAIBEL P E,NAHORNIAK N A,DZIUK H E,et al.Bioavailability of phosphorus in commercial phosphate supplements for turkeys[J].Poultry Science,1984,63(4):730-737.

[40] BOYD R D,HALL D,WU J F.Plasma alkaline phosphatase as a criterion for determining biological availability of phosphorus for swine[J].Journal of Animal Science,1983,57(2):396-401.

[41] SANDS J S,RAGLAND D,BAXTER C,et al.Phosphorus bioavailability,growth performance,and nutrient balance in pigs fed high available phosphorus corn and phytase[J].Journal of Animal Science,2001,79(8):2134-2142.

[42] MILLER E R,ULLREY D E,ZUTAUT C L,et al.Phosphorus requirement of the baby pig[J].Journal of Nutrition,1964,82(1):34-40.

[43] KOCH M E,MAHAN D C,CORLEY J R.An evaluation of various biological characteristics in assessing low phosphorus intake in weanling swine[J].Journal of Animal Science,1984,59(6):1546-1556.

[44] ANGEL R,SAYLOR W W,MITCHELL A D,et al.Effect of dietary phosphorus,phytase,and 25-hydroxycholecalciferol on broiler chicken bone mineralization,litter phosphorus,and processing yields[J].Poultry Science,2006,85(7):1200-1211.

[45] 董作一,何姝颖.不同无机磷源在生长育肥猪上的相对生物学效价[J].饲料广角,2016(17):25-29.

[46] 罗士津,瞿明仁,张铁鹰.低磷日粮对猪生长性能、骨骼及血液指标的影响[J].中国畜牧业,2015(20):77-79.

[47] 涂广运.福美双诱导条件下磷源和水平对肉鸡生产性能和胫骨质量的影响[D].硕士学位论文.雅安:四川农业大学,2012.

[48] RUCKER R B,PARKER H E,ROGLER J C.Utilization of calcium and phosphorus from hydrous and anhydrous dicalcium phosphates[J].Journal of Nutrition,1969,96(4):513-518.

[49] KEMME P A,RADCLIFFE J S,JONGBLOED A W,et al.Factors affecting phosphorus and calcium digestibility in diets for growing-finishing pigs[J].Journal of Animal Science,1997,75(8):2139-2146.

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