Molecular Nutrition

Study on Optimum Requirement for Phosphorus and Relative Biological Availability of High Soluble Monodicalcium Phosphate of Largemouth Bass (Micropterus salmoides)

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  • 1. National Aquafeed Safety Assessment Station, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
    3. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2017-11-20

  Online published: 2018-09-20

Abstract

In this experiment, various levels of monocalcium phosphate (MCP) and high soluble monodicalcium phosphate (MDCP) were added to low-phosphorus basal diet, in order to study the effects of them on growth performance, apparent digestibility coefficients of phosphorus (ADCp) and nitrogen (ADCn), retention ratios of phosphorus (PRR) and nitrogen value (NRR), phosphorus content of vertebra and whole body, and ash content of whole body, and to determine the optimum requirement for phosphorus and the biological availability of high soluble MDCP relative to MCP of largemouth bass (Micropterus salmoides). A low-phosphorus basal diet was prepared firstly, and then 8.86, 11.08, 13.29 and 15.51 g/kg of MCP, and 9.34, 11.67, 14.01 and 16.34 g/kg of high soluble MDCP were added into the low-phosphorus basal diet to equally supply 2.0, 2.5, 3.0 and 3.5 g/kg of phosphorus, respectively. The nine experimental diets were accordingly named P0, P2, P2.5, P3.0, P3.5 and DP2.0, DP2.5, DP3.0, DP3.5. Each diet was fed to four replicates (tanks) with 40 largemouth bass[initial body weight:(8.53±0.01) g] in each tank for 8 weeks. The results showed as follows:diets supplemented with different levels of MCP and high soluble MDCP both significantly increased the weight gain rate (WGR), ADCp, PRR and whole body ash content of the largemouth bass (P<0.05), but there were no significant differences in them between the two phosphorus sources (P>0.05). With the MCP supplemental level increasing, the WGR and whole body ash content were increased firstly and then went in a stabilization trend, the PRR was increased firstly and then declined, and all of them in P0 group were the lowest. With the high soluble MDCP supplemental level increasing, the WGR and ADCp showed a upward tendency, the PRR was increased firstly and then decreased, and the lowest and the highest values were observed in P0 and DP3 groups, respectively (P<0.05). The biological availability of high soluble MDCP relative to MCP is 100% and 118% based on the WGR and PRR through slope method, respectively. With MCP as the source of phosphorus, the WGR and PRR reach the highest when dietary digestible phosphorus content is 0.51%. With high soluble MDCP as the source of phosphorus, the PRR reached the highest when dietary digestible phosphorus content is 0.52%.

Cite this article

SHI Bo, YU Huanhuan, ZHENG Yinhua, WU Xiufeng, CHEN Pei, LIANG Xufang, XUE Min . Study on Optimum Requirement for Phosphorus and Relative Biological Availability of High Soluble Monodicalcium Phosphate of Largemouth Bass (Micropterus salmoides)[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3377 -3387 . DOI: 10.3969/j.issn.1006-267x.2018.09.005

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