Establishment of Oxidative Damage Model of Dairy Cow Peripheral Blood Mononuclear Cells Induced by Diethylenetriamine/Nitric Oxide Adduct

ZHENG Yaguang, QI Jingyu, ZHANG Boqi, SHI Binlin, YAN Sumei

doi:10.3969/j.issn.1006-267x.2018.09.021

2018 , Vol. 30 >Issue 9: 3517 - 3523

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.09.021

Molecular Nutrition

Establishment of Oxidative Damage Model of Dairy Cow Peripheral Blood Mononuclear Cells Induced by Diethylenetriamine/Nitric Oxide Adduct

Expand

College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2018-02-09

Online published: 2018-09-20

Fold

Abstract

This study was conducted to investigate the suitable condition for oxidative damage model of dairy cow peripheral blood mononuclear cells (PBMC) induced by diethylenetriamine/nitric oxide adduct (DETA/NO) which was a nitric oxide (NO) donor. The oxidative damage model of PBMC was established by detecting the cell survival rate and antioxidant parameters. The test was divided into 2 parts. Test 1 was conducted as a single factor randomized arrangement, PBMC was exposed in DETA/NO with different concentrations[0 (control), 50, 100, 200, 300 and 500 μmol/L] for 2, 4, 6, 8 and 12 hours at 37℃, respectively. The suitable action time of DETA/NO was determined by detecting the cell survival rate. Based on the results of suitable action time of DETA/NO in test 1, the cells exposed in DETA/NO with different concentrations[0 (control), 50, 100, 200, 300 and 500 μmol/L] in test 2, the suitable action concentration of DETA/NO was further screened by detecting the antioxidant parameters and inflammatory cytokine contents. The results showed that the PBMC survival rate decreased to 72.3%, the activities of superoxide dismutase, catalase and glutathione peroxidase decreased significantly (P<0.05), and the contents of interleukin-1, interleukin-6, tumor necrosis factor-α, malondialdehyde and NO increased significantly (P<0.05) after treated with 200 μmol/L DETA/NO for 4 hours. It is concluded that the suitable action concentration and action time at 37℃ of DETA/NO for establishing the oxidative damage model of dairy cow PBMC are 200 μmol/L and 4 hours, respectively.

Key words： peripheral blood mononuclear cell; diethylenetriamine/nitric oxide adduct; oxidative damage

Cite this article

ZHENG Yaguang, QI Jingyu, ZHANG Boqi, SHI Binlin, YAN Sumei . Establishment of Oxidative Damage Model of Dairy Cow Peripheral Blood Mononuclear Cells Induced by Diethylenetriamine/Nitric Oxide Adduct[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3517 -3523 . DOI: 10.3969/j.issn.1006-267x.2018.09.021

References

[1] SORDILLO L M,MAVANGIRA V.The nexus between nutrient metabolism,oxidative stress and inflammation in transition cows[J].Animal Production Science,2014,54(9):1204-1214.

[2] ABUELO A,HERNÁNDEZ J,BENEDITO J L,et al.The importance of the oxidative status of dairy cattle in the periparturient period:revisiting antioxidant supplementation[J].Journal of Animal Physiology and Animal Nutrition,2015,99(6):1003-1016.

[3] SCHMIDT H H H W,WALTER U.NO at work[J].Cell,1994,78(6):919-925.

[4] FÖRSTERMANN U.Nitric oxide and oxidative stress in vascular disease[J].Pflügers Archiv-European Journal of Physiology,2010,459(6):923-939.

[5] 郭咏梅,张博綦,石惠宇,等.二乙烯三胺/一氧化氮聚合物诱导的奶牛乳腺上皮细胞氧化损伤模型的建立[J].动物营养学报,2016,28(8):2378-2384.

[6] JABLONSKA E,PUZEWSKA W,MARCI NCZYK M,et al.iNOS expression and NO production by neutrophils in cancer patients[J].Archivum Immunologiae et Therapiae Experimentalis,2005,53(2):175-179.

[7] 李俊良,史彬林,闫素梅,等.不同壳聚糖浓度培养液对断奶仔猪外周血淋巴细胞中花生四烯酸代谢的影响[J].动物营养学报,2014,26(1):184-189.

[8] KIM J,PARK S,JUNG C M,et al.A case of cycloserine-induced lichenoid drug eruption supported by the lymphocyte transformation test[J].Allergy,Asthma & Immunology Research,2017,9(3):281-284.

[9] 潘会君,唐宁,华晓东,等.中药调控一氧化氮合酶-一氧化氮系统的研究[J].中国实验方剂学杂志,2010,16(12):202-205.

[10] ALDERTON W K,COOPER C E,KNOWLES R G.Nitric oxide synthases:structure,function and inhibition[J].Biochemical Journal,2001,357(3):593-615.

[11] KEEFER L K,NIMS R W,DAVIES K M,et al."NONOates"(1-substituted diazen-1-ium-1,2-diolates) as nitric oxide donors:convenient nitric oxide dosage forms[J].Methods in Enzymology,1996,268:281-293.

[12] 周丽娜,叶文博.野木瓜注射液及其提取物对脊髓神经元的氧化保护和生长促进作用[J].上海师范大学学报(自然科学版),2011,40(5):540-545.

[13] HUO R,SHI Y,XU J J,et al.Antioxidant effect of human selenium-containing single-chain Fv in rat cardiac myocytes[J].Chemical Research in Chinese Universities,2009,25(2):216-219.

[14] 孙婧陶,李兆华,张宝修,等.过氧化氢诱导延边奶山羊乳腺上皮细胞氧化损伤模型的建立[J].江苏农业科学,2013,41(10):149-152.

[15] 叶新平,彭涛,苏智雄,等.暴露于低浓度乙醇下体外淋巴细胞氧化损伤模型的建立[J].现代预防医学,2010,37(8):1514-1516.

[16] INAL M E,KANBAK G,SUNAL E.Antioxidant enzyme activities and malondialdehyde levels related to aging[J].Clinica Chimica Acta,2001,305(1/2):75-80.

[17] LINDQVIST D,DHABHAR F S,JAMES S J,et al.Oxidative stress,inflammation and treatment response in major depression[J].Psychoneuroendocrinology,2017,76:197-205.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References