Primary Culture and Passage Method of Preadipocytes of Cashmere Lambs and Adult Goats

ZHANG Qingyue, WANG Xue, LIU Shulin, YU Yang, GUO Xiaoyu, YAN Sumei

doi:10.3969/j.issn.1006-267x.2018.09.025

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 9: 3551 - 3558

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.09.025

Molecular Nutrition

Primary Culture and Passage Method of Preadipocytes of Cashmere Lambs and Adult Goats

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Collage of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2018-02-27

Online published: 2018-09-20

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Abstract

The study aimed to explore the primary culture and passage methods of preadipocytes of cashmere lambs and adult goats, and to provide a cell model for further researching the metabolic mechanism of adipocytes of cashmere goats. Perirenal adipose tissues of 3-month-old Albas cashmere lambs were selected as test materials. Preadipocytes of lambs were obtained directly by collagenase digestion method, and identified by morphological observation, growth curve and oil red O staining; perirenal adipose tissues of cashmere adult goats were selected as test materials. Mature adipocytes of adult goats were obtained by collagenase digestion and "ceiling" culture methods, and then preadipocytes were obtained by the dedifferentiation of mature adipocytes. After induction of differentiation, oil red O staining was used to identify their differentiation into mature adipocytes. The appropriate separation conditions for preadipocytes of the perirenal adipose tissues of lambs were digestion by 0.1% Ⅰ collagenase at 37℃ for 1 h, and centrifugation at 250×g for 10 min; during cell passage, digestion by 0.25% trypsin for 60 s. The preadipocytes were fusiform, grew in an "S" shape, and were positive on oil red O staining. Mature adipocytes of adult goats were obtained by collagenase digestion method in the same way as the lambs' method, and then using modified "ceiling" method obtained preadipocytes. After induction of differentiation, those preadipocytes were positive on oil red O staining. In conclusion, it is feasible to obtain preadipocytes directly by type Ⅰ collagenase digestion method from 3-month-old Albas cashmere lambs and by collagenase digestion and modified "ceiling" methods from adult cashmere goats.

Key words： cashmere adult goats; cashmere lames; preadipocytes; cell culture

Cite this article

ZHANG Qingyue, WANG Xue, LIU Shulin, YU Yang, GUO Xiaoyu, YAN Sumei . Primary Culture and Passage Method of Preadipocytes of Cashmere Lambs and Adult Goats[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3551 -3558 . DOI: 10.3969/j.issn.1006-267x.2018.09.025

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