Molecular Nutrition

Dietary Lipid Level Affects Growth Performance, Serum Biochemical Indexes and Mucin Gene Expression of Juvenile Blunt Snout Bream (Megalobrama amblycephala)

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  • 1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;
    2. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;
    3. Tongwei Co., Ltd., Chengdu 610000, China

Received date: 2018-02-27

  Online published: 2018-09-20

Abstract

This study was aimed to investigate the effects of dietary lipid level on growth performance, serum biochemical indexes and mucin gene expression of juvenile blunt snout bream (Megalobrama amblycephala). A total of 540 juvenile blunt snout breams with an initial average body weight of (10.0±0.5) g were randomly divided into 6 groups with 3 replicates in each group and 30 fish in each replicate and fed isonitrogenous and isoenergetic diets containing 2.29%, 4.29%, 6.29%, 8.29%, 10.29% and 12.29% lipid, respectively. After 8-week feeding trail, the growth, serum biochemical indexes and mucin gene expression in skin and intestine were detected. The results showed as follows:1) the weight gain rate (WGR) in 6.29% and 8.29% groups was significantly higher than that in other groups (P<0.05), while the feed conversion ratio (FCR) was significantly lower than that of other groups (P<0.05). 2) Cortisol content and alanine transaminase (ALT) and aspartate transaminase (AST) activities in serum in 12.29% group were the highest, and significantly higher than those in 2.29%, 4.29%, 6.29% and 8.29% groups (P<0.05), but not significant in 10.29% group (P>0.05). 3) The activities of superoxide dismutase (SOD), catalase (CAT) and GSH content in liver in 8.29% group were significantly higher than those in 2.29% group (P<0.05); glutathione reductase (GR) activity in 6.29% was the highest, and significantly higher than that in other groups except for 8.29% group (P<0.05); malonaldehyde (MDA) content in 6.29% group was the lowest, and significantly lower than that in 2.29% and 12.29% groups (P<0.05). 4) The intestinal mucin 2 (Muc2) gene expression level in 12.29% group was significantly higher than that in other groups (P<0.05); there was no significant difference in the skin mucin 5b (Muc5b) gene expression level between 8.29% and 12.29% groups(P>0.05), but significantly higher than other groups (P<0.05). 5) After challenged, the death ratio in 2.29% and 12.29% groups was the highest and that in 6.29% and 8.29% groups was the lowest, and was significantly lower than that in other groups (P<0.05). In conclusion, under this experiment conditions, when the dietary lipid levels are 6.29% to 8.29%, juvenile blunt snout bream have the best growth performance, disease-resistant ability, and skin Muc5b gene expression level.

Cite this article

XI Bingwen, LU Chunyun, REN Mingchun, MI Haifeng, XIE Jun . Dietary Lipid Level Affects Growth Performance, Serum Biochemical Indexes and Mucin Gene Expression of Juvenile Blunt Snout Bream (Megalobrama amblycephala)[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3559 -3566 . DOI: 10.3969/j.issn.1006-267x.2018.09.026

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