Effects of Dietary Arachidonic Acid Level on Growth Performance, Antioxidant Ability, Serum Biochemical Parameters and Fatty Acid Composition in Liver and Muscle of Juvenile Hybrid Grouper (Epinephelus fuscoguttatus♀&times;Epinephelus lanceolatus♂)

WANG Chengqiang, WANG Jiying, HUANG Bingshan, LI Baoshan, SUN Yongzhi, WANG Xiaoyan, MA Changxing

doi:10.3969/j.issn.1006-267x.2018.09.027

2018 , Vol. 30 >Issue 9: 3567 - 3580

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.09.027

Molecular Nutrition

Effects of Dietary Arachidonic Acid Level on Growth Performance, Antioxidant Ability, Serum Biochemical Parameters and Fatty Acid Composition in Liver and Muscle of Juvenile Hybrid Grouper (Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)

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1. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;
2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

Received date: 2018-03-07

Online published: 2018-09-20

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Abstract

An 8-week feeding experiment was conducted to evaluate the effects of dietary arachidonic acid (ARA) level on growth performance, antioxidant ability, serum biochemical parameters and fatty acid compo sition in liver and muscle of juvenile hybrid grouper (Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus♂). Six isonitrogenous and isoenergetic diets were formulated with the ARA level of 0.04%, 0.17%, 0.35%, 0.66%, 1.29% and 2.16% (dry matter basis) (named S1, S2, S3, S4, S5 and S6, separately), respectively. Each diet had 3 replicates and each replicate cultured 30 juvenile hybrid grouper with an initial body weight of (23.77±0.98) g. The results showed as follows:1) the specific growth rate (SGR) and feed efficiency (FE) increased at first, and then decreased with the increase of dietary ARA level, which reaching their peaks at S4 group (P<0.05), and significantly higher than S1 group (P<0.05). The body and liver ether extract content of S3 group was the lowest, and significantly lower than that of S5 and S6 groups (P<0.05). 2) The activities of superoxide dismutase (SOD) and catalase (CAT), total antioxidant capacity (T-AOC) in liver of S4 group were no significant differences with S3 group (P>0.05), but significantly higher than those of S1 and S6 groups (P<0.05). The malondialdehyde (MDA) content in liver of S3 and S4 groups was significantly lower than that of S1 and S6 groups (P<0.05). 3) The lowest values of the activities of aspartate aminotransferase (AST), cereal third transaminase (ALT) and alkaline phosphatase (AKP) in serum were found in S4 group, and significantly lower than those of S1 and S6 groups (P<0.05). 4) The contents of C20:3n-6 and C20:4n-6 in liver and muscle were significantly increased with the increase of dietary ARA level, but the contents of C18:3n-6, C20:5n-3 and C22:6n-3 were decreased with different extents. These results indicate that optimal dietary ARA level (0.35% to 0.66%) can improve the growth of juvenile hybrid grouper, and increase the antioxidant ability and liver health level. The broken-line model analysis based on SGR and FE indicate that the optimal level of dietary ARA is 0.45% and 0.56% of diet on dry matter basis for juvenile hybrid grouper, respectively.

Key words： Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂; arachidonic acid; growth performance; serum biochemical parameters; fatty acid composition

Cite this article

WANG Chengqiang, WANG Jiying, HUANG Bingshan, LI Baoshan, SUN Yongzhi, WANG Xiaoyan, MA Changxing . Effects of Dietary Arachidonic Acid Level on Growth Performance, Antioxidant Ability, Serum Biochemical Parameters and Fatty Acid Composition in Liver and Muscle of Juvenile Hybrid Grouper (Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3567 -3580 . DOI: 10.3969/j.issn.1006-267x.2018.09.027

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