Molecular Nutrition

Effects of Feeding Frequency on Growth Performance, Body Composition, Digestive Enzyme Activities, Tissue Fatty Acid and Amino Acid Compositions of Sepia pharaonis

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  • Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2018-02-28

  Online published: 2018-09-20

Abstract

This experiment was conducted to investigate the effects of feeding frequency on growth performance, body composition, digestive enzyme activities, tissue fatty acid and amino acid compositions of Sepia pharaonis. A total of 180 juvenile Sepia pharaonis with an initial body weight of (13.08±0.01) g were randomly divided into 3 groups (feeding frequencies were 1, 2 and 4 times a day, named F1, F2 and F4 groups, respectively), and each group had 3 replicates of 20 Sepia pharaonis per replicate. The experiment lasted for 4 weeks. The results showed as follows:1) the weight gain rate (WGR), specific growth rate (SGR) and hepatosomatic index (HSI) in F2 and F4 groups were significantly higher than those in F1 group (P<0.05), however, the feed efficiency (FE) in F1 and F4 groups was significantly lower than that in F2 group (P<0.05). 2) The liver moisture content in F4 group was significantly lower than that in F1 group (P<0.05), and the liver crude lipid content was significantly higher than that in F1 and F2 groups (P<0.05). The muscle moisture content in F2 and F4 groups was significantly lower than that in F1 group (P<0.05), and the muscle crude protein and crude lipid contents was significantly higher than that in F1 group (P<0.05). 3) There were no significant differences in the contents of total amino acids (TAA), essential amino acids (EAA) and non-essential amino acids (NEAA) in muscle among all groups (P>0.05). The contents of TAA, EAA and NEAA in liver in F1 group were significantly lower than those in F2 and F4 groups (P<0.05), the contents of TAA and NEAA in liver in F1 group had no significant difference compared with F4 group (P>0.05). 4) The docosahexaenoic acid (DHA) content and DHA/eicosapentaenoic acid (EPA) in muscle and liver in F1 group were significantly higher than those in F2 and F4 groups (P<0.05), and there were no significant differences in DHA content and DHA/EPA in muscle and liver between F2 and F4 groups (P>0.05). There were no significant differences in the contents of polyunsaturated fatty acids (PUFA) and n-3 high unsaturated fatty acids (n-3HUFA) in muscle and liver among all groups (P>0.05). 5) The liver glutathione peroxidase activity in F1 group was significantly higher than that in F2 group (P<0.05). The activities of α-amylase, lipase and alkaline phosphatase in anterior intestine in F1 group were significantly higher than those in F2 group (P<0.05), but no significant differences were found between F2 and F4 groups (P>0.05). According to the experimental results, the appropriate feeding frequency for juvenile Sepia pharaonis is 2 times a day.

Cite this article

ZHU Tingting, LI Chenchen, LU You, JIN Min, WANG Xuexi, LUO Jiaxiang, ZHOU Qicun . Effects of Feeding Frequency on Growth Performance, Body Composition, Digestive Enzyme Activities, Tissue Fatty Acid and Amino Acid Compositions of Sepia pharaonis[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3581 -3592 . DOI: 10.3969/j.issn.1006-267x.2018.09.028

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