Effects of Lysine on Expression of Genes Related to Lactose Synthesis in Bovine Mammary Epithelial Cells

CHEN Lu, ZHAO Yanli, GUO Xiaoyu, SHI Binlin, YAN Sumei

doi:10.3969/j.issn.1006-267x.2018.09.029

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 9: 3593 - 3598

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.09.029

Molecular Nutrition

Effects of Lysine on Expression of Genes Related to Lactose Synthesis in Bovine Mammary Epithelial Cells

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College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2018-02-14

Online published: 2018-09-20

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Abstract

The objective of this study was to determine the effect of lysine (Lys) on the expression of genes related to lactose synthesis in bovine mammary epithelial cells (BMECs), and to discuss the mechanism of the effect of Lys on lactose synthesis. The third generation BMECs were divided into 6 groups with 6 repetitions, and were cultured in the medium at concentrations of 0.5 (control), 1.0, 2.0, 4.0, 8.0 and 16.0 mmol/L Lys, respectively, for 48 h cultivation at 37℃ and 5% CO₂. The results showed as follows:the proper concentration of Lys had a significant dose-dependent effect on the content of lactose and the expression levels of glucose transporter 1 (GLUT1), hexokinase Ⅰ (HKⅠ) and hexokinase Ⅱ (HKⅡ) genes (P<0.05); the variance analysis results showed that increasing Lys concentration had a significant or tend to significant effect on the content of lactose and the expression of genes related to lactose synthesis. Compared with the control, the content of lactose in 2.0 to 16.0 mmol/L Lys groups was higher (0.05 < P < 0.10), the expression level of GLUT1 gene in 4.0 to 16.0 mmol/L Lys groups increased significantly (P<0.05), the expression levels of α-lactalbumin (LALBA) and β-1,4-galactosyltransferase 1 (β-4GALT1) genes in 2.0 to 8.0 mmol/L Lys groups increased significantly (P<0.05), and the expression level of HKⅡ gene in 8.0 to 16.0 mmol/L Lys groups increased significantly (P<0.05); the expression level of HKⅡ gene in 1.0 to 2.0 mmol/L Lys groups was inhibited significantly (P<0.05); the expression of HKⅠ gene in 4.0 to 16.0 mmol/L Lys groups was inhibited, especially for that in 16.0 mmol/L Lys group, which was significantly lower than that in the control (P<0.05). In conclusion, it shows that the Lys significantly improves the content of lactose, GLUT1, HKⅠ and HKⅡ genes expression in dose-dependent manner and the optimal Lys concentrations for lactose synthesis is 2.0 to 8.0 mmol/L.

Key words： dairy cow; bovine mammary epithelial cells; lysine; lactose; gene expression

Cite this article

CHEN Lu, ZHAO Yanli, GUO Xiaoyu, SHI Binlin, YAN Sumei . Effects of Lysine on Expression of Genes Related to Lactose Synthesis in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3593 -3598 . DOI: 10.3969/j.issn.1006-267x.2018.09.029

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