Molecular Nutrition

Effects of Lipopolysaccharide Inflammation Time on Blood Immunity and Intestinal Morphology of Mice

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  • College of Animal Sciences and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2018-02-28

  Online published: 2018-09-20

Abstract

This experiment was conducted to detect the changes in blood cytokine levels and intestinal tissue morphology of mice by intraperitoneal injection of lipopolysaccharide (LPS). Twenty-five male ICR mice were intraperitoneally injected with LPS (5 mg/kg BW), and the blood were sampled to prepare serum at different time points of inflammation[before LPS injection (0 h) and 3, 6, 12, 24, 48 and 72 h after injection]. The activity of intestinal alkaline phosphatase (IAP) and the levels of inflammatory cytokine, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-4, IL-6 and IL-8 in serum of mice were detected by enzyme-linked immunosorbent assay (ELISA) method. The samples of duodenum, jejunum, ileum and cecum were collected from the mice and hematoxylin-eosin (HE) staining was performed to observe the pathological changes of each intestinal segment. The villous height (VH) and crypt depth (CD) in each intestinal segment were measured and the VH/CD(V/C) was calculated. The results showed as follows:as time progressed, the activity of serum IAP and the levels of serum pro-inflammatory cytokines TNF-α, IL-6 and IL-8, and anti-inflammatory cytokines IL-4 peaked at 6 h after LPS injection, and then decreased continuously. The levels of IL-1β, a pro-inflammatory cytokine, increased slightly at 6 h after LPS injection and peaked at 48 h after injection. Compared with 0 h before LPS injection, the intestinal pathological changes of LPS-induced mice at 6 h after LPS injection were obvious. The mucous membranes of intestine were damaged, the epithelium was detached, the villus was broken, the mucous membranes were atrophic, edema, and the villus was short; over time, intestinal mucosa had slowly recovered at 48 h after LPS injection. Compared with 0 h before LPS injection, V/C in the duodenum and jejunum of LPS-inflamed mice were significantly decreased at 6 h after injection (P<0.05 or P<0.01); however, there was no significant difference at 72 h after injection (P>0.05). It is concluded that the levels of serum pro-inflammatory cytokines peaked at 6 h after intraperitoneal injection of LPS (5 mg/kg BW), intestinal mucosal damage is obvious, and gradually returns to normal afterwards.

Cite this article

JIA Junfeng, WANG Mengzhu, CUI Yizhe, WANG Qiuju, WU Rui . Effects of Lipopolysaccharide Inflammation Time on Blood Immunity and Intestinal Morphology of Mice[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3609 -3616 . DOI: 10.3969/j.issn.1006-267x.2018.09.031

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