Molecular Nutrition

Effects of Compound Additive with Benzoic acid, Bacillus coagulans and Oregano Oil on Growth Performance, Antioxidant Capacity and Jejunal Digestion-Absorption Function of Piglets Challenged with Enterotoxigenic Escherichia coli

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  • 1. Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China;
    2. Animal Biotechnology Center of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 610043, China

Received date: 2018-03-06

  Online published: 2018-09-20

Abstract

This experiment was conducted to investigate the effects of compound additive with benzoic acid, Bacillus coagulans and oregano oil on growth performance, antioxidant capacity, jejunal mucosa disaccharidase activities and nutrient transporter mRNA expression of piglets challenged with enterotoxigenic Escherichia coli. Twenty (24±1)-day-old Duroc×Landrace×Yorkshire castration piglets with body weight of (7.64±0.46) kg were randomly assigned to four groups with five replicates per group and one pig per replicate. Piglets in control group (CON group) and enterotoxigenic Escherichia coli-challenged group (ETEC group) were fed basal diets, and others in antibiotic group (AT group) and compound additives group (ABO group) were fed basal diets supplemented with antibiotics (20 g/t colistin sulfate+40 g/t bacitracin zinc) and compound additives (3 000 g/t benzoic acid+400 g/t Bacillus coagulans+400 g/t oregano oil), respectively. On day 22, all piglets were orally infused with 3×1011 CFU Escherichia coli, except piglets in CON group, which were received the same dose of sterile essential medium. The experiment lasted for 26 days. The results showed that compared with CON group, the diarrhea rate and diarrhea index of piglets in ETEC group were significantly increased (P<0.05), the content of malondialdehyde (MDA) in serum and jejunal mucosa was significantly increased(P<0.05), the total antioxidant capability (T-AOC) and total superoxide dismutase(T-SOD) activity in serum and jejunal mucosa sodium-glucose cotransporter 1 (SGLT1) mRNA expression levels were significantly decreased (P<0.05), and tended to decrease the T-AOC and the T-SOD activity in jejunal mucosa (P<0.10). Compared with ETEC group, the average daily gain (ADG) of piglets in ABO group was significantly increased (P<0.05), the feed to gain ratio (F/G) of piglets was significantly decreased (P<0.05), the diarrhea rate and diarrhea index of piglets were significantly decreased (P<0.05), the content of MDA in serum and jejunal mucosa were significantly decreased (P<0.05), the T-AOC and T-SOD activity in serum and jejunal mucosa were significantly increased (P<0.05), the jejunal mucosa SGLT1 and oligopeptide transporter 1 (PepT1) mRNA expression level were significantly increased (P<0.05). Furthermore, compared with AT group, the diarrhea index of piglets in ABO group was significantly decreased (P<0.05), the serum T-AOC was significantly increased (P<0.05). In conclusion, dietary supplementation with the compound additive with benzoic acid, Bacillus coagulans and oregano oil can relieve the diarrhea of piglets challenged with Escherichia coli, improve the antioxidant capacity of piglets, and improve growth performance and jejunal digestion-absorption function.

Cite this article

PU Junning, CHEN Daiwen, TIAN Gang, HE Jun, ZHENG Ping, MAO Xiangbing, YU Jie, HUANG Zhiqing, LUO Junqiu, LUO Yuheng, ZHU Ling, YU Bing . Effects of Compound Additive with Benzoic acid, Bacillus coagulans and Oregano Oil on Growth Performance, Antioxidant Capacity and Jejunal Digestion-Absorption Function of Piglets Challenged with Enterotoxigenic Escherichia coli[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3652 -3661 . DOI: 10.3969/j.issn.1006-267x.2018.09.036

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