Molecular Nutrition

Antibacterial Activities of Six Phenolic Compounds and Juglone in Vitro

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  • 1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
    2. College of Food and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2018-02-27

  Online published: 2018-09-20

Abstract

Extracts from walnut (Juglans regia L.) green husks showed a good antibacterial effect. In this experiment, the antibacterial activities of seven plant secondary metabolites with higher content in walnut green husks and leaves were evaluated to provide references for resolving the antibacterial activity of walnut green husk extracts and development of new feed antibiotics substitutes. Escherichia coli (E. coli), Bacillus subtilis (B. subtilis) and Staphylococcus aureus (S. aureus) were used as the tested bacteria. The seven plant secondary metabolites, chlorogenic acid, ellagic acid, syringic acid, rutin, tannic acid, p-hydroxybenzoic acid and juglone, were diluted at the concentrations of 1.000 0, 0.500 0, 0.250 0, 0.125 0 and 0.062 5 mg/mL, respectively. The Kirby-Bauer test was adopted to evaluate the antibacterial activities of the seven plant secondary metabolites by the diameter of inhibition zone after 24 h culture. The results showed that E.coli was insensitive to p-hydroxybenzoic acid and tannic acid, while the other plant secondary metabolites showed different extent inhibitory effects on the 3 tested bacteria. For the E. coli, the inhibitory effect was in the rank order of ellagic acid > juglone > rutin=syringic acid > chlorogenic acid > p-hydroxybenzoic acid=tannic acid. The order of inhibitory effect on B. subtilis was ellagic acid > chlorogenic acid > p-hydroxybenzoic acid > syringic acid > tannic acid > rutin > juglone. Meanwhile, the order of inhibitory effect on S. aureus was juglone > syringic acid > rutin > tannic acid > ellagic acid > p-hydroxy benzoic acid > chlorogenic acid. In summary, chlorogenic acid, ellagic acid, syringic acid, rutin, tannic acid, p-hydroxybenzoic acid and juglone have different extent inhibitory effects on E. coli, B. subtilis and S. aureus. Ellagic acid has a strong inhibitory effect on E. coli and B. subtilis, and juglone has a strong inhibitory effect on E. coli and S. aureus.

Cite this article

YNAG Guang, BAO Xiaowei, CHEN Yong, WU Ying, MA Jingjing . Antibacterial Activities of Six Phenolic Compounds and Juglone in Vitro[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3710 -3719 . DOI: 10.3969/j.issn.1006-267x.2018.09.042

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