Molecular Nutrition

Screening of Fumonisin B1 Aptamer and Development of Its Rapid and Quantitative Assay

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  • 1. College of Life Science, Northeast Agricultural University, Harbin 150030, China;
    2. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Biotechnology, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin 150069, China;
    3. College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang 157011, China

Received date: 2018-03-02

  Online published: 2018-09-20

Abstract

This experiment was conducted to obtain fumonisin B1 aptamers and to develop a rapid test method based the effect of fumonin B1 and colloidal gold. Used the systematic evolution of ligands by exponential enrichment technology of non-immobilized target to screen the fumonisin B1 aptamers, whose affinity and specificity was determined by enzyme-linked immunosorbent assay (ELISA) method, and to develop a colloidal gold rapid and quantitative test based on aptamers. The results showed that the affinity dissociation constant of the aptamer F102 of fumonisin B1 was (42.1±4.0) nmol/L, and the fumonisin B1 colloidal gold detection method was simple, rapid and specific. The relative error of fumonisin B1 colloidal gold detection results and high performance liquid chromatography detection results was less than 15.7%. Therefore, the fumonisin B1 aptamers screened by the experiment can be used for testing the fumonisin B1 content in cereals and feedstuffs rapidly and quantitative.

Cite this article

ZHOU Yan, DIAO Chenxi, ZHANG Yuanyuan, LU Taofeng, ZHAO Lili, CHEN Hongyan . Screening of Fumonisin B1 Aptamer and Development of Its Rapid and Quantitative Assay[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3740 -3750 . DOI: 10.3969/j.issn.1006-267x.2018.09.046

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