Effects of Glucocorticoid and Dietary Fat Level on Lipid Metabolism of Broilers

WANG Minghui, LIU Zhimei, WANG Haoqi, GUAN Jiajia, HU Qingmei, WANG Xiaojuan

doi:10.3969/j.issn.1006-267x.2018.09.049

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 9: 3772 - 3780

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.09.049

Molecular Nutrition

Effects of Glucocorticoid and Dietary Fat Level on Lipid Metabolism of Broilers

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Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science, Shandong Agricultural University, Tai'an 271018, China

Received date: 2018-02-12

Online published: 2018-09-20

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Abstract

This study aimed to research the effects of glucocorticoid[dexamethasone (DEX)] and dietary fat level on lipid metabolism of broilers. A total of 200 Arbor Acre broilers with similar body weight were exposed to diets with high (HFD) or low fat level (LFD) from 22 days of age, each treatment had 100 broilers. The diets were fed from 22 to 38 days of age. At 35 to 38 days of age, broilers in each dietary treatment were divided into two sub-treatments, and broilers in which were subcutaneous injected DEX (2 mg/kg BW) and saline with equal volume. Each sub-treatment had 50 broilers. The results showed as follows:1) under the conditions of LFD and HFD, glucocorticoid both significantly decreased body weight and thigh muscle weight (P<0.05), and significantly raised liver proportion and blood concentration of triglyceride (TG) (P<0.05). 2) Under the condition of LFD, glucocorticoid significantly increased abdominal fat proportion and TG content in muscle (P<0.05). 3) Under the condition of HFD, glucocorticoid significantly increased carnitine palmitoyl transferase 1 (CPT1) content in abdominal fat and thigh muscle (P<0.05), while significantly decreased CPT1 content in liver and breast muscle (P<0.05). 4) There were overall up-regulated trends of expression levels of glucocorticoids receptor (GR), peroxisome proliferator-activated receptor α (PPARα) and fatty acid transport protein (FATP1) mRNAs in breast and abdominal fat after the treatment of glucocorticoid, while they were contrary for thigh muscle and liver. The results indicate that glucocorticoid may affect lipid metabolism through regulating GR, PPARα and FATP1, and facilitate the fat ectopic deposit in liver and muscle. High fat diet may activate CPT1 and lipid oxidation in abdominal fat and thigh muscle, thereby alleviate glucocorticoids' effect on fat deposition.

Key words： stress; glucocorticoids; dietary fat level; broiler; lipid metabolism

Cite this article

WANG Minghui, LIU Zhimei, WANG Haoqi, GUAN Jiajia, HU Qingmei, WANG Xiaojuan . Effects of Glucocorticoid and Dietary Fat Level on Lipid Metabolism of Broilers[J]. Chinese Journal of Animal Nutrition, 2018 , 30(9) : 3772 -3780 . DOI: 10.3969/j.issn.1006-267x.2018.09.049

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