This experiment was to study the effects of different calcium level diets supplemented with vitamin D₃ on performance, egg quality, tibia quality and plasma calcium and phosphorus metabolism of laying hens in late laying period. A 3×3 factorial design was adopted in this experiment to design 3 calcium levels (3.0%, 3.5% and 4.0%)and 3 vitamin D₃ supplemental levels (0, 2 500 and 5 000 IU/kg). Eight hundred and ten 60-week-old healthy Lohmann laying hens were randomly assigned to 9 groups with 6 replicates per group and 15 hens per replicate. The corn-soybean meal diet was used in this experiment. The pre-trial period was 1 week and the official trial period was 6 weeks. The results showed as follows:1) compared with the group without adding vitamin D₃, the average daily feed intake and laying rate of hens in the vitamin D₃ supplemental group were extremely significantly increased (P<0.01), and the ratio of feed to egg, break egg rate and soft shell egg rate were extremely significantly decreased (P<0.01). With the increasing of dietary calcium level and vitamin D₃ supplemental level, the average weight of egg was increased, and the rate of dirty egg was decreased, but the difference was not significant (P>0.05). 2) Compared with the group without adding vitamin D₃, the eggshell strength, eggshell weight and eggshell proportion in the vitamin D₃ supplemental group were extremely significantly increased (P<0.01), the eggshell thickness was significantly increased (P<0.05), and the yolk weight was significantly reduced (P<0.05). With the increasing of dietary calcium level, the eggshell strength showed an increasing trend, but it was not significant (P<0.05). 3) Effects of different calcium level diets supplemented with vitamin D₃ on tibia strength were extremely significant (P<0.01). Compared with the group without adding vitamin D₃, the tibia strength in the vitamin D₃ supplemental group was extremely significantly increased (P<0.01). With the increasing of dietary calcium level, the calcium content in the tibia showed an increasing trend, but it was not significant (P<0.05). When the dietary calcium level was 4.0%, the tibia strength was significantly higher than that when the dietary calcium levels were 3.0% and 3.5% (P<0.05). 4) Compared with the group without adding vitamin D₃, the plasma alkaline phosphatase activity of hens in the vitamin D₃ supplemental group was extremely significantly decreased (P<0.01), and the contents of plasma calcium and phosphorus were extremely significantly increased (P<0.01). With the increasing of dietary calcium level, there was no significant change in the contents of plasma calcium and phosphorus (P>0.05). Compared with the 3.0% calcium group, the plasma alkaline phosphatase activity in the 4.0% calcium group was significantly decreased (P<0.05). In conclusion, appropriately increasing dietary calcium level is beneficial to the performance of laying hens in late laying period, but deficiency of vitamin D₃ reduces performance, even causes production shutdown. Supplementation of 2 500 IU/kg vitamin D₃ level can meet the requirement of vitamin D₃ in the later stage of laying hens without causing waste of resources and saving cost.