Effects of Vitamin B12 Supplemental Level on Growth Performance, Intestinal Development and Microflora Structure in Cecum of Wulong Geese Aged from 5 to 15 Weeks

LONG Jianhua, WANG Baowei, KONG Min, ZHANG Ming, YUE Bin, GE Wenhua, KE Changjiao, DIAO Cuiping, SUN Lingling

doi:10.3969/j.issn.1006-267x.2018.10.017

2018 , Vol. 30 >Issue 10: 3930 - 3940

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.017

Molecular Nutrition

Effects of Vitamin B₁₂ Supplemental Level on Growth Performance, Intestinal Development and Microflora Structure in Cecum of Wulong Geese Aged from 5 to 15 Weeks

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National Aquatic Industry Technology System, Research Center of Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2018-03-27

Online published: 2018-10-20

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Abstract

This experiment was conducted to investigate the effects of vitamin B₁₂ supplemental level on growth performance, intestinal development and microflora structure in cecum of Wulong geese aged from 5 to 15 weeks, aimed to explore the optimum supplemental level of vitamin B₁₂ in diets of Wulong geese aged from 5 to 15 weeks. A total of 360 Wulong geese at the end of 4 weeks of age were selected and randomly divided into 6 groups with 6 replicates in each group and 10 geese in each replicate (half males and half females). Group Ⅰ was the control group, and geese in the control group were fed a basal diet without vitamin B₁₂ (measured vitamin B₁₂ content was 0 in the basal diet); groups Ⅱ to Ⅵ were the test groups, and geese in the test groups were fed the basal diet supplemented with 0.005 (group Ⅱ), 0.010 (group Ⅲ), 0.015 (group Ⅳ), 0.020 (group Ⅴ) and 0.025 mg/kg vitamin B₁₂ (group Ⅵ), respectively. The trial period was 11 weeks. The results showed as follows:1) the final body weight (FBW) of groups Ⅱ to Ⅳ was significantly or extremely significantly higher than that of control group (P<0.05 or P<0.01), and the average daily gain (ADG) of groups Ⅲ to Ⅳ was significantly or extremely significantly higher than that of control group (P<0.05 or P<0.01). 2) The villus height (VH) of test groups was significantly or extremely significantly higher than that of control group (P<0.05 or P<0.01), and the highest value was found in group Ⅲ. The crypt depth (CD) of test groups was significantly or extremely significantly lower than that of control group (P<0.05 or P<0.01), and the lowest value was found in group Ⅲ. The ratio of VH to CD (V/C) of test groups was significantly or extremely significantly higher than that of control group (P<0.05 or P<0.01), and the highest value was found in group Ⅲ. 3) Diets supplemented with different levels of vitamin B₁₂ could change the relative abundances of dominant microorganisms in class, family and genus levels. In the class level, Clostridia, Bacteroides and Deltaproteobacteria had obviously changes; in the family level, Bacteroidaceae, Ruminococcaceae and Desulfovibrionaceae had obviously changes; in the genus level, Bacteroides and Desulfovibrio had obviously changes. In conclusion, the optimum supplemental level of vitamin B₁₂ in diets can improve the growth performance and change the relative abundance of dominant microorganisms in cecum of Wulong geese aged from 5 to 15 weeks. It is recommended that the supplemental level of vitamin B₁₂ in the diet of Wulong geese aged from 5 to 15 weeks is 0.012 6 to 0.013 4 mg/kg with the FBW and ADG as evaluation indices.

Key words： vitamin B₁₂; Wulong geese; growth performance; intestinal development; cecum; microflora structure

Cite this article

LONG Jianhua, WANG Baowei, KONG Min, ZHANG Ming, YUE Bin, GE Wenhua, KE Changjiao, DIAO Cuiping, SUN Lingling . Effects of Vitamin B₁₂ Supplemental Level on Growth Performance, Intestinal Development and Microflora Structure in Cecum of Wulong Geese Aged from 5 to 15 Weeks[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 3930 -3940 . DOI: 10.3969/j.issn.1006-267x.2018.10.017

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