Molecular Nutrition

Effects of Adding Calcium Folate in Rumen Protected Trytophan Diets on Plasma Tryptophan, Kynurenine and Melatonin Contents in Sheep

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  • Xinjiang Agricultural University, Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Urumqi 830052, China

Received date: 2018-04-07

  Online published: 2018-10-20

Abstract

The objective of this study was to explore the method of regulating the synthesis of melatonin (MT) in ruminants by detecting the effects of adding two doses of calcium folate (CF) in rumen protected trytophan (RPTrp) diets on plasma tryptophan (Trp), kynurenine (Kyn) and MT contents in sheep. Fifteen Suffolk sheep aged (3.0±0.5) years, with an average body weight (64.45±2.41) kg were divided into 3 groups (5 in each group):control group, trail group Ⅰ and trial group Ⅱ. The amount of feed (per day per sheep) were 12 g/kg BW of concentrate supplement, 1.8 kg corn silage, 222.2 mg/kg BW of RPTrp, and mixed hay was fed ad libitum. On the basis of this, sheep in trail group Ⅰ and trial group Ⅱ were fed 50 and 100 mg CF per sheep per day, respectively. The results showed as follows:1) there was no significant difference in plasma Trp and Kyn contents among groups during 0 to 12.0 h after feeding in the morning (P>0.05), but plasma Trp content in trial groups reduced at 6.0 and 8.0 h after feeding in the morning (P=0.087 2, P=0.053 1). Plasma Kyn content in the plasma of trial groups reduced too at 4.5, 6.0, 8.0 and 10.0 h after feeding in the morning (P=0.094 8, P=0.066 7, P=0.090 9, P=0.0542). 2) At 4.5 and 8.0 h after feeding in the morning, plasma 5-hydroxytryptamin (5-HT) content in trail groups increased compared with the control group (P=0.080 7, P=0.054 1). At 10.0 h after feeding in the morning, plasma 5-HT content in trail groups significantly increased (P=0.005 7). At 6.0 and 8.0 h after feeding in the morning, plasma MT content in trail groups also increased (P=0.089 0, P=0.070 4), and it was significantly higher at 10.0 h after feeding in the morning than that in the control group (P=0.000 2). 3) Compared with the control group, the plasma total antioxidant capacity and glutathione peroxidase activity in trail group Ⅱ were significantly increased (P<0.05), malonaldehyde content was significantly decreased (P<0.05), and total antioxidant capacity in trail group Ⅰ was also significantly improved (P<0.05). In conclusion, the RPTrp (222.2 mg/kg BW) diets with 50 or 100 mg CF per sheep per day has the tendency of reducing plasma Kyn content from 4.5 to 10.0 h after feeding in the morning, has no significant effect on plasma Trp, 5-HT, MT contents, but can improve the plasma antioxidant capacity of sheep.

Cite this article

WANG Gen, CHEN Hui, ZHAO Fang, GAO Chao, ZHAO Guodong, LI Xiaobin, MA Chen, YANG Kailun . Effects of Adding Calcium Folate in Rumen Protected Trytophan Diets on Plasma Tryptophan, Kynurenine and Melatonin Contents in Sheep[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 3968 -3975 . DOI: 10.3969/j.issn.1006-267x.2018.10.021

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