Effects of Periodical Starvation-Refeeding on Growth Performance, Antioxidant Indices, Digestive Enzyme Activities, Amino Acid Composition and Fatty Acid Composition of Juvenile Sepia pharaoni

LI Chenchen, ZHU Tingting, LU You, LUO Jiaxiang, JIN Min, YUAN Ye, ZHOU Qicun

doi:10.3969/j.issn.1006-267x.2018.10.024

2018 , Vol. 30 >Issue 10: 3993 - 4004

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.024

Molecular Nutrition

Effects of Periodical Starvation-Refeeding on Growth Performance, Antioxidant Indices, Digestive Enzyme Activities, Amino Acid Composition and Fatty Acid Composition of Juvenile Sepia pharaoni

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Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2018-03-23

Online published: 2018-10-20

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Abstract

A 14-day feeding trial was conducted to evaluate the effects of periodical starvation-refeeding on growth performance, antioxidant indices, digestive enzyme activities, amino acid composition and fatty acid composition of juvenile Sepia pharaoni. A total of 360 juvenile Sepia pharaoni with an initial body weight of (6.83±0.01) g were randomly divided into 4 groups with 3 replicates per group and 30 Sepia pharaoni for each replicate. In the experiment, four different repetitive cycles of starvation-refeeding modes were used, they were starvation 1 day and refeeding 6 days (S1F6 group), starvation 2 days and refeeding 5 days (S2F5 group), starvation 3 days and refeeding 4 days (S3F4 group) and continuous feeding (control group). Results showed as follows:1) the weight gain ratio (WGR), specific growth rate (SGR) and survival ratio were significantly affected by different starvation time, and above three indicators of the S3F4 group were significantly lower than control group (P<0.05), but those had no significant differences among the control group, S1F6 group and S2F5 group (P>0.05). 2) No significant differences in the moisture, crude protein and crude lipid contents in muscle among groups (P>0.05). 3) Hepatic superoxide dismutase (SOD) activity was significantly affected by different starvation time (P<0.05), and its highest value was found in S2F5 group. Hepatic glutathione peroxidase (GSH-Px) activity and the contents of glutathione (GSH) and malondialdehyde (MDA) were not significantly affected by different starvation time (P>0.05). 4) Hepatic amylase (AMS) and lipase (LPS) activities were affected by different starvation time (P<0.05). With the starvation time prolong, the hepatic AMS activity was decreased at first and then increased, and the lowest value was found in S1F6 group; while the hepatic LPS was increased at first, next decreased and then increased, and the lowest value was found in S2F5 group. 5) Seventeen kinds of amino acids were detected in muscle of juvenile Sepia pharaoni, and the contents of 10 kinds of essential amino acids and 7 kinds of non-essential amino acids, total amino acids (TAA), total essential amino acids (TEAA) and TEAA/TAA were not significantly affected by different starvation time (P>0.05). 6) The contents of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA) in liver and muscle were not significantly different among groups (P>0.05), and the contents of C20:5n-3 (EPA), C22:5n-3 (DPA) and C22:6n-3 (DHA) in muscle, and the contents of DPA and DHA in liver were also not significantly different among groups (P>0.05), but the hepatic EPA and n-3 PUFA contents in S3F4 group was significantly lower than that in control group (P<0.05). In conclusion, compensatory growth is observed in the juvenile Sepia pharaoni of S1F6 and S2F5 group, and the muscle conventional nutrients have no significant changes. Synthesizes the cost saving, reduce pollution and compensatory growth, we that the feeding mode of periodical starvation 2 days and refeeding 5 days can be suggested to use in juvenile Sepia pharaoni culture.

Key words： juvenile Sepia pharaoni; starvation-refeeding; conventional nutrients; digestive enzyme activities; amino acids; fatty acids

Cite this article

LI Chenchen, ZHU Tingting, LU You, LUO Jiaxiang, JIN Min, YUAN Ye, ZHOU Qicun . Effects of Periodical Starvation-Refeeding on Growth Performance, Antioxidant Indices, Digestive Enzyme Activities, Amino Acid Composition and Fatty Acid Composition of Juvenile Sepia pharaoni[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 3993 -4004 . DOI: 10.3969/j.issn.1006-267x.2018.10.024

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