Effects of Epidermal Growth Factor on Intestinal Type Ⅱb Sodium-Phosphate Cotransporter Expression of Weaned Piglets Challenged by Lipopolysaccharide

TANG Xiaopeng, XU Rong, LI Chengliang, PENG Peng, YU Qifang, FANG Rejun

doi:10.3969/j.issn.1006-267x.2018.10.027

2018 , Vol. 30 >Issue 10: 4020 - 4027

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.027

Molecular Nutrition

Effects of Epidermal Growth Factor on Intestinal Type Ⅱb Sodium-Phosphate Cotransporter Expression of Weaned Piglets Challenged by Lipopolysaccharide

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College of Animal Science and Technology, Hunan Agricultural University, Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China

Received date: 2018-03-29

Online published: 2018-10-20

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Abstract

The aim of this experiment was to study the effects of epidermal growth factor (EGF) on intestinal type Ⅱb sodium-phosphate cotransporter (NaPi-Ⅱb) expression of weaned piglets under stress condition challenged by lipopolysaccharide (LPS). The study was consisted of two parts, 1) cell experiment:intestinal porcine intestinal epithelial cells (IPEC-J2) were as the experimental model, and divided into 4 groups with 3 replicates in each, control group (0 ng/mL EGF, 0 μg/mL LPS), EGF group (100 ng/mL EGF, 0 μg/mL LPS), LPS group (0 ng/mL EGF, 1.0 μg/mL LPS), EGF+LPS group (100 ng/mL EGF, 1.0 μg/mL LPS); 2) animal experiment:twenty-four 21-day-old healthy weaned piglets (Large White×Landrace) with an average body weight of (5.76±0.38) kg were randomly divided into 4 groups, control group (basal diet), EGF group (basal diet+2.0 mg/kg EGF), LPS group (basal diet+intraperitoneal injection of 100 mg/kg LPS), EGF+LPS group (basal diet+2.0 mg/kg EGF+intraperitoneal injection of 100 mg/kg LPS), with 6 replicates per group and 1 piglet in each replicate. The results showed as follows:1) in the cell experiment, compared with the control group, the expression of NaPi-Ⅱb mRNA and protein in IPEC-J2 cells was significantly decreased in EGF group (P<0.05), whereas, that in IPEC-J2 cells was significantly increased in EGF+LPS group (P<0.05), and there was a significant interaction between EGF and LPS immunological stress on the expression of NaPi-Ⅱb mRNA and protein (P<0.05); 2) in the animal experiment, there was no significant difference in serum Ca content among all groups (P>0.05), serum P content in LPS group was significantly higher than that in control group, EGF group, and EGF+LPS group (P<0.05), alkaline phosphatase (ALP) activity in EGF group was significantly higher than that in LPS group (P<0.05), there was a significant interaction between EGF and LPS immunological stress on serum P content (P<0.05), but not on Ca content and ALP activity (P>0.05). Compared with the control group, the expression of NaPi-Ⅱb mRNA in the jejunum and ileum in EGF group was significantly decreased (P<0.05), while, that in EGF+LPS group was significantly increased (P<0.05), and there was a significant interaction between EGF and LPS immunological stress on the expression of NaPi-Ⅱb mRNA in the jejunum and ileum (P<0.05). The results of cell experiment and animal experiment show that the EGF can inhibit the NaPi-Ⅱb expression, but can improve NaPi-Ⅱb expression under immunological stress condition, which indicate that EGF can promote the active absorption of P.

Key words： epidermal growth factor; phosphorus; NaPi-Ⅱb; lipopolysaccharide; porcine intestinal epithelial cells; weaned piglets

Cite this article

TANG Xiaopeng, XU Rong, LI Chengliang, PENG Peng, YU Qifang, FANG Rejun . Effects of Epidermal Growth Factor on Intestinal Type Ⅱb Sodium-Phosphate Cotransporter Expression of Weaned Piglets Challenged by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 4020 -4027 . DOI: 10.3969/j.issn.1006-267x.2018.10.027

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