Molecular Nutrition

Effects of Mannan Oligosaccharides on Rumen Microflora Structure of Dairy Cows Based on 16S rDNA High-Throughput Sequencing Technology

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  • Collage of Agriculture, Ningxia University, Yinchuan 750021, China

Received date: 2018-03-14

  Online published: 2018-10-20

Abstract

This experiment was conducted to study the effects of mannan oligosaccharides on rumen microflora structure of dairy cows based on 16S rDNA high-throughput sequencing technology. Four healthy Chinese Holstein lactating dairy cows with the same parity in the similar lactation period were randomly divided into 2 groups. Cows in control group were fed a basal diet and those in experimental group were fed the basal diet with 60 g/cow mannan oligosaccharides through oral perfusion. The experiment was designed through a 2×2 cross-over test with 21 days in each stage (14 days for pre-experiment and 7 days for samples collection). The results showed as follows:1) compared with the control group, adding mannose oligosaccharides significantly increased acetic acid concentration in the rumen (P<0.01), and acetic acid/propionic acid also increased by 8.47% (P>0.05). 2) Compared with the control group, at the phylum level, the relative abundance of Cyanobacteria in experimental group decreased significantly (P<0.01), and that of Armatimonadetes increased significantly (P<0.01). At the genus level, the relative abundance of Anaerobiospirillum in experimental group significantly increased (P<0.05), and that of Clostridium significantly decreased (P<0.05). The relative abundance of Pyramidobacter decreased significantly (P<0.01). The relative abundances of Ruminococcus, Pseudobutyrivibrio and Lachnospira increased by 24.34%, 12.83% and 31.80% (P>0.05), respectively. The relative abundance of Prevotella reduced by 4.66%, and that of Veillonellaceae_NA and Desulfovibrio increased by 143.11% and 4.88% (P>0.05), respectively, In this experiment, adding mannan oligosaccharide reduces the diversity of rumen bacteria of dairy cows, which obviously affects cellulolytic bacteria flora, hemicellulose-degrading bacteria flora and significantly increases the concentration of acetic acid in rumen fluid,and increases pH in rumen fluid, but has no significant effect.

Cite this article

GUO Tingting, HU Dandan, FU Zilin, LI Na, XU Xiaofeng . Effects of Mannan Oligosaccharides on Rumen Microflora Structure of Dairy Cows Based on 16S rDNA High-Throughput Sequencing Technology[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 4048 -4058 . DOI: 10.3969/j.issn.1006-267x.2018.10.030

References

[1] GIANNENAS I,DOUKAS D,KARAMOUTSIOS A,et al.Effects of Enterococcus faecium,mannan oligosaccharide,benzoic acid and their mixture on growth performance,intestinal microbiota,intestinal morphology and blood lymphocyte subpopulations of fattening pigs[J].Animal Feed Science and Technology,2016,220:159-167.

[2] 唐志如,孙志洪,印遇龙,等.环保型饲料添加剂-寡糖的作用机理[J].中国畜牧兽医,2003,30(4):17-19.

[3] 陈小兵,丁宏标,乔宇.甘露寡糖的益生作用、免疫机制与应用技术[J].中国畜牧兽医,2005,32(8):6-8.

[4] PICCOLO G,CENTODUCATI G,MARONO S,et al.Effects of the partial substitution of fish meal by soy bean meal with or without mannanoligosaccharide and fructooligosaccharide on the growth and feed utilization of sharpsnout seabream,Diplodus puntazzo (Cetti,1777):preliminary results[J].Italian Journal of Animal Science,2016,10(3):e37.

[5] 祁茹,肖宇,褚永康,等.外源寡糖对反刍动物胃肠道微生物区系的影响[J].饲料博览,2011(12):32-34.

[6] HEINRICHS A J,JONES C M,HEINRICHS B S.Effects of mannan oligosaccharide or antibiotics in neonatal diets on health and growth of dairy calves[J].Journal of Dairy Science,2003,86(12):4064-4069.  

[7] 刘立恒,闵力,瞿明仁,等.甘露寡糖、果寡糖和大豆寡糖组合对锦江黄牛瘤胃液细菌多样性的影响[J].动物营养学报,2012,24(08):1583-1588.

[8] 冯仰廉,周建民,张晓明,等.我国奶牛饲料产奶净能值测算方法的研究[C]//第三届全国反刍动物营养学术研讨会论文集.北京:中国畜牧兽医学会,2011:6.

[9] 徐晓锋.缩合单宁与水解单宁对奶牛日粮氮利用影响及作用机制的研究[D].博士学位论文.北京:中国农业大学,2013.

[10] YU Y,LEE C,KIM J,et al.Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction[J].Biotechnology & Bioengineering,2010,89(6):670-679.

[11] SAR C,SANTOSO B,MWENYA B,et al.Manipulation of rumen methanogenesis by the combination of nitrate with β,1-4 galacto-oligosaccharides or nisin in sheep[J].Animal Feed Science and Technology,2004,115(1/2):129-142.

[12] LEY R E,LOZUPONE C A,HAMADY M,et al.Worlds within worlds:evolution of the vertebrate gut microbiota[J].Nature Reviews Microbiology,2008,6(10):776-788.  

[13] OLIVEIRA M L S,ARÊAS A P M,CAMPOS I B,et al.Induction of systemic and mucosal immune response and decrease in Streptococcus pneumoniae colonization by nasal inoculation of mice with recombinant lactic acid bacteria expressing pneumococcal surface antigen A[J].Microbes and Infection,2006,8(4):1016-1024.  

[14] QIN J J,LI R Q,RAES J,et al.A human gut microbial gene catalogue established by metagenomic sequencing[J].Nature,2010,464(7285):59-65.  

[15] SINGH K M,AHIR V B,TRIPATHI A K,et al.Metagenomic analysis of Surti buffalo (Bubalus bubalis) rumen:a preliminary study[J].Molecular Biology Reports,2012,39(4):4841-4848.  

[16] 闵力.功能性寡糖组合对锦江黄牛瘤胃微生物区系的影响[D].硕士学位论文.南昌:江西农业大学,2012.

[17] DERAKHSHANI H.The microbiome composition of the rumen is altered during the peripartal period in dairy cattle[C]//2014 ADSA-ASAS-CSAS Joint annual meeting.Kansas City:ASAS,2014.

[18] LEE C Y K.The first insights into the phylogeny,genomics,and ecology of the novel bacterial phylum armatimonadetes[D].Ph.D.Thesis.New Zealand:University of Waikato,Hamilton,2015.

[19] ASCHENBACH J R,PENNER G B,STUMPFF F,et al.Ruminant nutrition symposium:role of fermentation acid absorption in the regulation of ruminal pH[J].Journal of Animal Science,2011,89(4):1092-1107.  

[20] ZEBELI Q,ASCHENBACH J R,TAFAJ M,et al.Invited review:role of physically effective fiber and estimation of dietary fiber adequacy in high-producing dairy cattle[J].Journal of Dairy Science,2012,95(3):1041-1056.  

[21] PURUSHE J,FOUTS D E,MORRISON M,et al.Comparative genome analysis of Prevotella ruminicola and Prevotella bryantii:insights into their environmental niche[J].Microbial Ecology,2010,60(4):721-729.  

[22] 裴彩霞.反刍动物瘤胃微生物及其利用[M].北京:中国科学技术出版社,2013:9-10.

[23] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2006:4-5.
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