Molecular Nutrition

Analysis of Intestinal Microbiota Diversity of Blue Foxes by High-Throughput Sequencing Technology

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  • Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130117, China

Received date: 2018-03-30

  Online published: 2018-10-20

Abstract

The aim of this experiment was to study the composition and diversity of intestinal microbiota of blue foxes (Alopex lagopus). Eight healthy growing female blue foxes (5 to 6 months of age) under the stall-feeding condition were selected to collect fresh faecal samples, and then the composition and diversity of intestinal microbiota were analyzed by the high-throughput sequencing technology. The results showed as follows:a total of 569 930 valid sequences were identified in faecal samples from 8 healthy blue fox. The numbers of operational taxonomic unit (OUT) ranged from 468 to 574, representing 16 phyla and 209 genera. The dominant bacterial phyla in turn were Firmicutes (62.97%), Bacteroidtes (22.05%), Actinobacteria (8.89%), Proteobacteri (5.15%) and Fusobacteria (0.88%). The dominant bacterial genera in turn were Streptococcus (11.75%), Lactobacillus (9.86%), Prevotella (9.28%), Megasphaera (8.21%), Collinsella (7.27%), Blautia (7.08%) and Bacteroides (5.64%). The present study represents that there is a complex intestinal microbiota structure of blue foxes by the high-throughput sequencing technology, and there were bigger differences among individuals.

Cite this article

CHEN Shuangshuang, SI Huazhe, MU Linlin, LI Guangyu, LIU Hanlu . Analysis of Intestinal Microbiota Diversity of Blue Foxes by High-Throughput Sequencing Technology[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 4071 -4080 . DOI: 10.3969/j.issn.1006-267x.2018.10.032

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