UPLC-MS/MS Method for the Determination of Five &beta;-agonists Residue in Pork Liver, Beef and Mutton

ZHANG Suzhen, WANG Yijun, TIAN Yun, HE Yan, GE Min

doi:10.3969/j.issn.1006-267x.2018.10.034

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 10: 4087 - 4094

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.034

Molecular Nutrition

UPLC-MS/MS Method for the Determination of Five β-agonists Residue in Pork Liver, Beef and Mutton

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Lianyungang Municipal Quality Supervision and Inspection Center for Animal Products, Lianyungang 222000, China

Received date: 2018-03-20

Online published: 2018-10-20

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Abstract

The aim of this study was to establish a UPLC-MS/MS method for rapid determination of five β-agonists in pork liver, beef and mutton. The five β-agonists in samples were extracted with 80% acetonitrile, purified by PRiME HLB solid phase extraction column, concentrated by nitrogen, and finally dissolved with 10% methanol. A gradient elution was performed using 10 mmol/L ammonium acetate aqueous solution-10 mmol/L ammonium acetate methanol (pH=5) as a mobile phase. The multi-reaction monitoring (MRM) mode was used for the determination, and internal standard method was used for quantification. The concentration of five β-agonists showed good linearity in the range of 0.5 to 50.0 ng/mL with the coefficient correlation (R²) between 0.999 63 to 0.999 96. At the standard addition levels of 0.5, 1.0 and 5.0 μg/kg, the average recovery ratio of five β-agonists was 93.7% to 106.1% in pork liver, 95.2% to 104.0% in beef, and 95.1% to 106.0% in mutton. The test indicated that the method showed good accuracy and repeatability. Based on S/N=3, the detection limit of five β-agonists was 0.01 to 0.04 μg/kg, and quantitation limit was 0.02 to 0.15 μg/kg. This method is simple, sensitive, accurate, and suitable for large quantities animal products detection analysis.

Key words： UPLC-MS/MS; β-agonists; animal products

Cite this article

ZHANG Suzhen, WANG Yijun, TIAN Yun, HE Yan, GE Min . UPLC-MS/MS Method for the Determination of Five β-agonists Residue in Pork Liver, Beef and Mutton[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 4087 -4094 . DOI: 10.3969/j.issn.1006-267x.2018.10.034

References

[1] 康升云.β受体激动剂对畜产品安全的影响[J].中国畜牧兽医文摘,2012,28(7):211

[2] 王培龙.β-受体激动剂及其检测技术研究[J].农产品质量与安全,2014(1):44-52.

[3] 魏凤静.关于"瘦肉精"分类、危害及检测标准的探讨[J].山东畜牧兽医,2017,38(4):59-60.

[4] COURTHEYN D,LE BIZEC B,BRAMBILLA G,et al.Recent developments in the use and abuse of growth promoters[J].Analytica Chimica Acta,2002,473(1/2):71-82.

[5] 沈建忠,江海洋.畜产品中β-受体激动剂残留及其危害[J].中国动物检疫,2011,28(6):27-28.

[6] 叶妮,孙雷,尹晖,等.UPLC-MS/MS法检测动物性食品中19种β-受体激动剂残留[J].中国兽药杂志,2015,49(9):51-59.

[7] 聂婉,伍晓红,陈秋玲,等.β-兴奋剂性质及其最新检测方法研究进展[J].黑龙江畜牧兽医,2016(23):75-77.

[8] 熊琳,李维红,高雅琴,等.肉品中β-受体激动剂类药物残留检测技术研究进展[J].食品安全质量检测学报,2015,6(2):528-533.

[9] 朱坚,李波,方晓明,等.气相色谱-质谱法测定肝、肾和肉中11种β-受体激动剂残留量[J].质谱学报,2005,26(3):129-137.

[10] 张旖,赵善贞,曲栗,等.HPLC-q/LTQ-MS法测定肉及肉制品中10种β-受体激动剂[J].食品科学,2014,35(20):202-207.

[11] MAURO D,CIARDULLO S,CIVITAREALE C,et al.Development and validation of a multi-residue method for determination of 18β-agonists in bovine urine by UPLC-MS/MS[J].Microchemical Journal,2014,115:70-77.

[12] WANG P L,LIU X M,SU X O,et al.Sensitive detection of β-agonists in pork tissue with novel molecularly imprinted polymer extraction followed liquid chromatography coupled tandem mass spectrometry detection[J].Food Chemistry,2015,184:72-79.

[13] PAN S D,ZHOU L X,ZHAO Y G,et al.Development and validation of a sensitive method for simultaneous determination of eight β2-agonists in pork by ultrasonic-assisted extraction and liquid chromatography/tandem mass spectrometry[J].Chromatographic Science,2015,53(1):104-111.

[14] SUNG I K,PARK S J,KANG K,et al.Development and application of a method for rapid and simultaneous determination of three β-agonists (clenbuterol,ractopamine,and zilpaterol) using liquid chromatography-tandem mass spectrometry[J].Korean Journal for Food Science of Animal Resources,2015,35(1):121-129.

[15] 周帅,张飞云.UPLC-MS/MS检测运动食品中β-受体激动剂残留[J].食品研究与开发,2017,38(11):148-152.

[16] 招钰娟,邓晓华,甘沛明,等.液相色谱串联质谱法测定动物组织中17种β-兴奋剂残留[J].安徽农业科学,2017,45(15):95-99.

[17] 刘佳,梁桂荣,李少晖,等.液相色谱-串联质谱法检测饲料中26种β2-受体激动剂类药物残留[J].食品安全质量检测学报,2015,6(4):1167-1173.

[18] 刘畅,吴小虎,徐伟东,等.LC-MS/MS测定动物源性食品中15种β-受体激动剂残留的研究[J].药物分析杂志,2008,28(12):2085-2089.

[19] 吉彩霓,岳振峰,欧阳姗.固相萃取-高效液相色谱串联质谱法同时测定猪肉中克伦特罗和沙丁胺醇残留[J].中国预防医学杂志,2006,40(2):123-125.

[20] 倪炜华.UPLC-MS/MS测定动物源食品中9种β-受体激动剂[J].化学分析计量,2017,26(3):67-71.

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