Molecular Nutrition

Effects of Dietary Fish Oil on Immune Function of Laying Hens

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  • Key Open Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, National Engineering Research Center of Biological Feed Development, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2018-04-10

  Online published: 2018-10-20

Abstract

This experiment was conducted to investigate the effects of dietary fish oil on humoral and cellular immune function of laying hens. Four hundred and fifty healthy Hy-Line Brown laying hens of 31-week-old were randomly allotted into 5 groups with 6 replicates per group and 15 hens per replicate. The laying hens in the control group were fed a corn-soybean meal type basal diet, and those in experimental groups were fed the basal diets supplemented with 0.54%, 1.08%, 2.17% and 4.34% fish oil with 0.68, 1.35, 2.70 and 5.40 mg/g docosahexaenoic acid, respectively. The feeding trial lasted for 12 weeks after 1 week of adaption. The results showed as follows:1) dietary fish oil did not significantly affect the spleen index, thymus index and serum immunoglobulin A content of laying hens (P>0.05). 2) The avian influenza virus H9 subtype antibody levels in serum of laying hens in experimental groups were significantly increased at 7 and 14 days after immunization compared with the control group (P<0.05), which in 2.17% fish oil group was the highest. At 28 days after immunization, the avian influenza virus H9 subtype antibody levels in serum of laying hens in experimental groups showed an increasing tendency compared with the control group (P<0.10). The Newcastle disease virus (NDV) antibody levels in serum of laying hens in experimental groups were significantly increased at 7 and 14 days after immunization compared with the control group (P<0.05), and at 28 days after immunization, the NDV antibody levels in serum of laying hens in experimental groups were not significantly different compared with the control group (P>0.05). 3) Compared with the control group, the relative gene expression levels of interleukin-2 and interleukin-6 in spleen of laying hens in 4.34% fish oil group were significantly decreased (P<0.05), and the relative gene expression levels of interleukin-10 and interferon-γ in spleen of laying hens in 1.08% and 2.17% fish oil groups were significantly increased (P<0.05). 4) Dietary fish oil had no significant effects on stimulus index and apoptosis rate of lymphocytes in spleen of laying hens except for 4.34% fish oil (P>0.05). In conclusion, dietary 0.54% to 2.17% fish oil can improve the immune function of laying hens but too high (4.34%) has no benefit.

Cite this article

LONG Shuo, WU Shugeng, CUI Chuangfei, ZHANG Haijun, WANG Jing, QI Guanghai, MA Youbiao, YANG Linlin . Effects of Dietary Fish Oil on Immune Function of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 4132 -4142 . DOI: 10.3969/j.issn.1006-267x.2018.10.039

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