Effects of Compound Enzyme Preparation on Rumen Fermentation, Apparent Digestibility of Nutrients and Performance of Lactating Cows

ZHAO Liansheng, WANG Dian, WANG Youyue, CHEN Yakun, BU Dengpan, LIU Wei, ZHENG Chen, GUO Jiangpeng

doi:10.3969/j.issn.1006-267x.2018.10.043

2018 , Vol. 30 >Issue 10: 4172 - 4180

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.043

Molecular Nutrition

Effects of Compound Enzyme Preparation on Rumen Fermentation, Apparent Digestibility of Nutrients and Performance of Lactating Cows

Expand

1. Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Inner Mongolia Youran Farming Co., Ltd., Hohhot 010070, China;
4. Beijing Animal Husbandry Environment Monitoring Station, Beijing 102200, China;
5. CAAS-ICRAF Joint Lab on Agroforestry and Sustainable Animal Husbandry, Beijing 100193, China;
6. Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China;
7. Beijing General Station of Animal Husbandry, Beijing 100107, China

Received date: 2018-03-26

Online published: 2018-10-20

Fold

Abstract

This experiment was conducted to investigate the effects of different doses of compound enzyme preparation on rumen fermentation, apparent digestibility of nutrients, serum biochemical indices and performance of lactating cows. Thirty-two healthy Holstein lactating cows were selected with similar body weight, parity, lactation days and milk yield. The cows were allocated to 4 groups according to a randomized blocks experiment design. Dairy cows in control group were fed a basal diet, while those in trial groups were fed the basal diet with 0.10%, 0.15% and 0.20% compound enzyme preparation, respectively. Preliminary trial period was 10 days, and formal trial period was 56 days. The results showed as follows:1) compared with the control group, adding 0.15% compound enzyme preparation in the diets significantly increased total volatile fatty acids content of rumen fluid (P<0.05) and adding compound enzyme preparation had a trend to increase acetic acid content (P=0.08), but had no significant effects on other volatile fatty acids, ammonia nitrogen and micro protein contents (P>0.05). 2) Compared with the control group, adding compound enzyme preparation in the diets significantly increased the apparent digestibility of dry matter and neutral detergent fiber (P<0.05), and adding 0.20% compound enzyme preparation significantly increased the apparent digestibility of organic matter (P<0.05). 3) Compared with the control group, adding compound enzyme preparation in the diets had a trend to increase serum albumin content (P=0.09) and decrease urea nitrogen content (P=0.07), but had no significant effects on other serum biochemical indexes (P>0.05). 4) Compared with the control group, adding 0.10% and 0.15% compound enzyme preparation in the diets significantly increased feed conversion rate (P<0.05), and the 3.5% milk fat correction milk yield in 0.10% and 0.15% groups was increased by 3.88 and 4.27 kg/d (P<0.05), respectively. The milk fat rate in 0.15% group was increased by 12.70% (P<0.05). In conclusion, adding compound enzyme preparation in the diets can improve feed conversion rate, rumen fermentation and performance. Comprehensive consideration, the recommended adding amount of compound enzyme preparation in the diets of lactating cows is 0.15%.

Key words： compound enzyme preparation; lactating cows; apparent digestibility; performance; serum biochemical indices

Cite this article

ZHAO Liansheng, WANG Dian, WANG Youyue, CHEN Yakun, BU Dengpan, LIU Wei, ZHENG Chen, GUO Jiangpeng . Effects of Compound Enzyme Preparation on Rumen Fermentation, Apparent Digestibility of Nutrients and Performance of Lactating Cows[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 4172 -4180 . DOI: 10.3969/j.issn.1006-267x.2018.10.043

References

[1] 何立荣,李爱华.酶制剂在奶牛日粮中的研究与应用进展[J].农业科学研究,2010,31(3):59-64.

[2] 周勃,冯杰.酶制剂和乳酸菌对育肥猪生产性能和养分消化率的影响[J].饲料与畜牧,2011(2):38-40.

[3] 赵鹏,唐德富,汝应俊,等.低小麦饲粮中添加酶制剂对肉仔鸡生产性能及养分消化利用的影响[J].甘肃农业大学学报,2015,50(1):25-30.

[4] MORGAVI D P,NEWBOLD C J,BEEVER D E,et al.Stability and stabilization of potential feed additive enzymes in rumen fluid[J].Enzyme and Microbial Technology,2000,26(2/3/4):171-177.

[5] 林静,赵鑫源,都文,等.复合酶制剂对泌乳奶牛瘤胃发酵、营养物质表观消化率及生产性能的影响[J].动物营养学报,2017,29(6):2124-2133.

[6] GADO H M,SALEM A Z M,ROBINSON P H,et al.Influence of exogenous enzymes on nutrient digestibility,extent of ruminal fermentation as well as milk production and composition in dairy cows[J].Animal Feed Science and Technology,2009,154(1/2):36-46.

[7] 韩兆玉,段智勇,丁立人,等.酶制剂对奶牛产奶量和乳品质的影响[J].粮食与饲料工业,2008(8):39-40.

[8] PETERS A,LEBZIEN P,MEYER U,et al.Effect of exogenous fibrolytic enzymes on ruminal fermentation and nutrient digestion in dairy cows[J].Archives of Animal Nutrition,2010,64(3):221-237.

[9] NRC.Nutrient requirements of dairy cattle[S].7th ed.Washington,D.C.:National Academy Press,2001.

[10] 国家技术监督局.GB/T 6432-1994饲料中粗蛋白测定方法[S].北京:中国标准出版社,1994.

[11] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 20806-2006饲料中中性洗涤纤维(NDF)的测定[S].北京:中国标准出版社,2007.

[12] 中华人民共和国农业部.NY/T 1459-2007饲料中酸性洗涤纤维的测定[S].北京:中国农业出版社,2008.

[13] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6438-2007饲料中粗灰分的测定[S].北京:中国标准出版社,2007.

[14] 王加启.反刍动物营养学研究方法[M].北京:现代教育出版社,2011.

[15] MAKKAR H P,BECKER K.Purine quantification in digesta from ruminants by spectrophotometric and HPLC methods[J].British Journal of Nutrition,1999,81(2):107-112.

[16] KEULEN J V,YOUNG B A.Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies[J].Journal of Animal Science,1977,44(2):282-287.

[17] 贾仙宝.外源纤维酶对奶牛瘤胃发酵、消化代谢及生产性能影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2009.

[18] 李朝云.壳聚糖与纤维素酶对瘤胃发酵、甲烷产生及微生物区系的影响[D].博士学位论文.杨凌:西北农林科技大学,2014.

[19] 陈宇.外源酶对湖羊瘤胃发酵的影响[D].硕士学位论文.福州:福建农林大学,2016.

[20] ARRIOLA K G,KIM S C,STAPLES C R,et al.Effect of fibrolytic enzyme application to low-and high-concentrate diets on the performance of lactating dairy cattle[J].Journal of Dairy Science,2011,94(2):832-841.

[21] BEAUCHEMIN K A,COLOMBATTO D C,MORGAVI D P,et al.Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants1,2[J].Journal of Animal Science,2003,81(14 Suppl.2):1061-1074.

[22] 呼和,金海,薛淑媛,等.外源纤维酶对奶牛瘤胃纤维降解细菌影响的研究[J].畜牧与饲料科学,2011,32(5):25-28.

[23] YANG W Z,BEAUCHEMIN K A,RODE L M.Effects of an enzyme feed additive on extent of digestion and milk production of lactating dairy cows[J].Journal of Dairy Science,1999,82(2):391-403.

[24] HRISTOV A N,MCALLISTER T A,CHENG K J.Effect of dietary or abomasal supplementation of exogenous polysaccharide-degrading enzymes on rumen fermentation and nutrient digestibility[J].Journal of Animal Science,1998,76(12):3146-3156.

[25] HRISTOV A N,MCALLISTER T A,CHENG K J.Stability of exogenous polysaccharide-degrading enzymes in the rumen[J].Animal Feed Science and Technology,1998,76(1/2):161-168.

[26] 徐学文,刘正亚,田雯,等.不同的复合酶制剂对奶牛生产性能和营养物质消化率的影响[J].粮食与饲料工业,2012,12(11):51-54.

[27] KNOWLTON K F,TAYLOR M S,HILL S R,et al.Manure nutrient excretion by lactating cows fed exogenous phytase and cellulase[J].Journal of Dairy Science,2007,90(9):4356-4360.

[28] 解祥学,杜红方,陈书琴,等.外源酶制剂在反刍动物上的应用与展望[J].动物营养学报,2016,28(4):1011-1019.

[29] CHOCT M.Enzymes for the feed industry:past,present and future[J].World's Poultry Science Journal,2006,62(1):5-16.

[30] 杨璐玲,吕永艳,孙国强.啤酒糟对奶牛产能性能及血液生化指标的影响[J].中国畜牧杂志,2014,50(13):51-56.

[31] 宋平.不同饲养方式下奶牛血液生化指标测定与分析[D].硕士学位论文.哈尔滨:东北农业大学,2007.

[32] 扈添琴.日粮中添加不同水平酶制剂与植物甾醇复合物对奶牛生产性能和血液指标的影响[D].硕士学位论文.南京:南京农业大学,2013.

[33] SILVA T H,TAKIYA C S,VENDRAMINI T H A,et al.Effects of dietary fibrolytic enzymes on chewing time,ruminal fermentation,and performance of mid-lactating dairy cows[J].Animal Feed Science and Technology,2016,221:35-43.

[34] SUTTON J D,PHIPPS R H,BEEVER D E,et al.Effect of method of application of a fibrolytic enzyme product on digestive processes and milk production in Holstein-Friesian cows[J].Journal of Dairy Science,2003,86(2):546-556.

[35] 杨泽坤.反刍动物专用复合酶制剂对奶牛瘤胃发酵、血液指标及生产性能的影响[D].硕士学位论文.杨凌:西北农林科技大学,2017.

[36] NSEREKO V L,BEAUCHEMIN K A,MORGAVI D P,et al.Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows[J].Canadian Journal of Microbiolog,2002,48(1):14-20.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References