Effects of Soybean Meal Replaced by Clostridium autoethanogenum Protein on Growth Performance, Plasma Biochemical Indexes and Hepatopancreas and Intestinal Histopathology of Grass Carp (Ctenopharyngodon idllus)

WEI Hongcheng, YU Huanhuan, CHEN Xiaoming, CHAO Wei, ZOU Fangqi, CHEN Pei, ZHENG Yinhua, WU Xiufeng, LIANG Xufang, XUE Min

doi:10.3969/j.issn.1006-267x.2018.10.045

2018 , Vol. 30 >Issue 10: 4190 - 4201

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.045

Molecular Nutrition

Effects of Soybean Meal Replaced by Clostridium autoethanogenum Protein on Growth Performance, Plasma Biochemical Indexes and Hepatopancreas and Intestinal Histopathology of Grass Carp (Ctenopharyngodon idllus)

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1. The National Aquatic Feed Safety Assessment Center, Feed Institute Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Beijing Shoulang Bio-Technology Co., Ltd., Beijing 100041, China;
3. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
4. Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, Beijing 100081, China

Received date: 2018-04-03

Online published: 2018-10-20

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Abstract

This experiment was conducted to evaluate the effects of soybean meal replaced by the new protein source Clostridium autoethanogenum protein on growth performance, plasma biochemical indexes and hepatopancreas and intestinal histopathology of grass carp (Ctenopharyngodon idllus). A total of 540 grass carp with an initial body weight of (25.66±0.03) g were randomly divided into 3 groups with 6 replicates per group and 30 fish per replicate. Fish in the control group (J0 group) were fed a basal diet, and others in the experimental groups were fed the experimental diets in which 5% (J5 group) and 10% (J10 group) Clostridium autoethanogenum protein replaced 27.5% and 55.0% soybean meal, respectively. The experiment lasted for 10 weeks. The results showed as follows:1) the survival rate of grass carp in J5 group was significantly higher than that in J10 group (P<0.05), while had no significant difference with J0 group (P>0.05). The weight gain rate of grass carp in J5 group was significantly higher than that in J0 group (P<0.05), and the feed conversion ratio was significantly lower than that in J0 group (P<0.05). 2) The plasma total cholesterol (TC) content of grass carp in J5 and J10 groups was significantly lower than that in J0 group (P<0.05); the plasma glucose content of grass carp in J10 group was significantly higher than that in J0 group (P<0.05); the plasma aspartate aminotransferase (AST) activity of grass carp in J10 group was significantly higher than that in J5 group (P<0.05), which in J5 group was significantly higher than that in J0 group (P<0.05); the plasma alanine aminotransferase (ALT) activity of grass carp in J10 group was significantly lower than that in J0 and J5 groups (P<0.05); the plasma AST/ALT in J10 group was significantly higher than that in J0 and J5 groups (P<0.05); the plasma malondialdehyde (MDA) content in J5 group was significantly lower than that in J0 and J10 groups (P<0.05). There were no significant differences in the contents of immunoglobulin M (IgM), interleukin-8 (IL-8) and interleukin-1α (IL-1α) in plasma among all groups (P>0.05). 3) The moisture content of whole fish in J10 group was significantly higher than that in J5 group (P<0.05). There were no significant differences in the contents of crude ash, crude protein and crude lipid of whole fish among all groups (P>0.05). 4) From the pathological sections of hepatopancreas and intestine, it was observed that with the increasing addition of Clostridium autoethanogenum protein, the nuclear accumulation in hepatopancreas increased, while the dorsal intestinal histology was improved compared with the control group. These results indicate that using 5% Clostridium autoethanogenum protein to replace of 27.5% soybean meal is beneficial to the growth performance of grass carp, but 10% addition would negatively affect the growth performance of grass carp, reduce the survival rate, and lead to liver damage. Therefore, the recommended addition of Clostridium autoethanogenum protein in diets for grass carp is 5%.

Key words： Clostridium autoethanogenum protein; grass carp; soybean meal; growth; histopathology

Cite this article

WEI Hongcheng, YU Huanhuan, CHEN Xiaoming, CHAO Wei, ZOU Fangqi, CHEN Pei, ZHENG Yinhua, WU Xiufeng, LIANG Xufang, XUE Min . Effects of Soybean Meal Replaced by Clostridium autoethanogenum Protein on Growth Performance, Plasma Biochemical Indexes and Hepatopancreas and Intestinal Histopathology of Grass Carp (Ctenopharyngodon idllus)[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 4190 -4201 . DOI: 10.3969/j.issn.1006-267x.2018.10.045

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