Effects of Dietary Replacement of Alfalfa Meal by Coix lacryma-jobi cv. Daheishan Meal on Intestinal Morphology, Digestive Enzyme Activities and Cecal Fermentation Parameters of Growing Meat Rabbits

TIAN Gang, LU Yuanyuan, YU Bing, ZENG Huijin, CHEN Daiwen, LUO Yuheng, CAI Jingyi

doi:10.3969/j.issn.1006-267x.2018.10.047

2018 , Vol. 30 >Issue 10: 4210 - 4218

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.10.047

Molecular Nutrition

Effects of Dietary Replacement of Alfalfa Meal by Coix lacryma-jobi cv. Daheishan Meal on Intestinal Morphology, Digestive Enzyme Activities and Cecal Fermentation Parameters of Growing Meat Rabbits

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Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2018-03-20

Online published: 2018-10-20

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Abstract

This experiment was conducted to investigate the effects of dietary different proportions Coixies lacryma-jobi cv. Daheishan meal replacement alfalfa meal on intestinal morphology, digestive enzyme activitives and cecal fermentation parameters of growing meat rabbits. A total of 100 weaned New Zealand white rabbits (35-day-old) with a similar weight were randomly assigned to 5 groups with 10 replicates per group and 2 rabbits per replicate. Rabbits in the control group (C group) and 4 replacement groups (S25, S50, S75 and S100 groups) were fed 5 experimental diets with different proportions (0, 25%, 50%, 75% and 100%) of alfalfa meal replaced by Coix lacryma-jobi cv. Daheishan meal. The experiment lasted for 15 days. On the 15th day of the experiment, 4 rabbits with an average body weight from each group were selected and slaughtered, and the tissue, mucous and digesta samples in small intestine and cecum were collected immediately for further analysis. The results indicated that there were no significant differences in the average daily intake, average daily gain and feed to gain ratio among all the groups (P>0.05). There were no significant differences in the villus height, crypt depth and the ratio of villous height to crypt depth in small intestine and crypt depth in cecum among all the groups (P>0.05). The duodenum sucrase activity in S50 group was significantly higher than that in S75 and S100 groups (P<0.05), however, there were no significant differences in the activities of other digestive enzymes in other intestinal tract among all groups (P>0.05), and the digestive enzyme activity was the highest in S50 group. There were no significant differences in the pH and contents of ammonia nitrogen, total volatile fatty acids, acetic acid, propionic acid and butyric acid, and acetic acid/(propionic acid+butyric acid) in cecum among all the groups (P>0.05). According to the results of 16S rRNA sequencing, the diversity of cecal microorganisms showed no difference at the phylum level and genus level between C group and S100 group. The results indicated that the dietary alfalfa meal replaced by the Coixlacryma-jobi cv. Daheishan meal has no significant effects on intestinal morphology, digestive enzyme activity and cecal fermentation parameters of growing meat rabbits, and the dietary alfalfa meal can be completely replaced by Coixlacryma-jobi cv. Daheishan meal, and the recommended substitution ratio is 50%.

Key words： Coix lacryma-jobi cv. Daheishan meal; growing meat rabbits; intestinal morphology; digestive enzyme activity; caecum fermentation

Cite this article

TIAN Gang, LU Yuanyuan, YU Bing, ZENG Huijin, CHEN Daiwen, LUO Yuheng, CAI Jingyi . Effects of Dietary Replacement of Alfalfa Meal by Coix lacryma-jobi cv. Daheishan Meal on Intestinal Morphology, Digestive Enzyme Activities and Cecal Fermentation Parameters of Growing Meat Rabbits[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 4210 -4218 . DOI: 10.3969/j.issn.1006-267x.2018.10.047

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